ID | 54416 |
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Author |
Setiawan, Heri
Department of Public Health, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Nagaoka, Kenjiro
Department of Public Health, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kubo, Masayuki
Department of Public Health, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kaken ID
Fujikura, Yoshihisa
Division of Morphological Analysis, Department of Anatomy, Biology and Medicine, Faculty of Medicine, Oita University
Ogino, Keiki
Department of Public Health, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
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Abstract | Oxidative stress is widely known to play a role in asthma. However, the contribution of xanthine oxidoreductase (XOR) as a source of the superoxide anion radical (O2-) in oxidative stress associated with asthma has not yet been examined in detail. Here we investigated pathophysiological changes in XOR in an experimental model of asthma induced by the house dust mite Dermatophagoides farinae (Df). In the lungs of Df-treated mice, the production of O2- from XOR increased and the nitrite concentrations decreased, whereas the protein expression of XOR remained unchanged. Moreover, the protein expression levels of XOR and the hydrogen peroxide (H2O2) concentrations in bronchoalveolar lavage fluid (BALF) were higher in the Df-treated mice than in saline-treated mice. Immunohistochemically, although XOR was highly localized in the bronchial epithelial cells of the saline-treated mice, immunostaining for XOR was absent in the bronchial epithelium of Df-treated mice. These results suggest that oxidative stress is up-regulated by increases in the conversion of the dehydrogenase form (xanthine dehydrogenase; XDH) of XOR to the oxidase form (xanthine oxidase; XOD).
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Keywords | xanthine oxidase
oxidative stress
asthma
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Amo Type | Original Article
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Publication Title |
Acta Medica Okayama
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Published Date | 2016-06
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Volume | volume70
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Issue | issue3
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Publisher | Okayama University Medical School
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Start Page | 175
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End Page | 182
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ISSN | 0386-300X
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NCID | AA00508441
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Content Type |
Journal Article
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language |
English
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Copyright Holders | CopyrightⒸ 2016 by Okayama University Medical School
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File Version | publisher
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Refereed |
True
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