JaLCDOI 10.18926/AMO/54416
FullText URL 70_3_175.pdf
Author Setiawan, Heri| Nagaoka, Kenjiro| Kubo, Masayuki| Fujikura, Yoshihisa| Ogino, Keiki|
Abstract Oxidative stress is widely known to play a role in asthma. However, the contribution of xanthine oxidoreductase (XOR) as a source of the superoxide anion radical (O2-) in oxidative stress associated with asthma has not yet been examined in detail. Here we investigated pathophysiological changes in XOR in an experimental model of asthma induced by the house dust mite Dermatophagoides farinae (Df). In the lungs of Df-treated mice, the production of O2- from XOR increased and the nitrite concentrations decreased, whereas the protein expression of XOR remained unchanged. Moreover, the protein expression levels of XOR and the hydrogen peroxide (H2O2) concentrations in bronchoalveolar lavage fluid (BALF) were higher in the Df-treated mice than in saline-treated mice. Immunohistochemically, although XOR was highly localized in the bronchial epithelial cells of the saline-treated mice, immunostaining for XOR was absent in the bronchial epithelium of Df-treated mice. These results suggest that oxidative stress is up-regulated by increases in the conversion of the dehydrogenase form (xanthine dehydrogenase; XDH) of XOR to the oxidase form (xanthine oxidase; XOD).
Keywords xanthine oxidase oxidative stress asthma
Amo Type Original Article
Published Date 2016-06
Publication Title Acta Medica Okayama
Volume volume70
Issue issue3
Publisher Okayama University Medical School
Start Page 175
End Page 182
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2016 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 27339206
Web of Science KeyUT 000379406100004
Author Zou, Yu| Wang, Da-Hong| Sakano, Noriko| Sato, Yoshie| Iwanaga, Suketaka| Taketa, Kazuhisa| Kubo, Masayuki| Takemoto, Kei| Masatomi, Chie| Inoue, Kiyomi| Ogino, Keiki|
Published Date 2014-02
Publication Title International Journal of Environmental Research and Public Health
Volume volume11
Issue issue2
Content Type Journal Article
JaLCDOI 10.18926/AMO/52652
FullText URL 68_3_129.pdf
Author Minoura, Akira| Wang, Da-Hong| Sato, Yoshie| Zou, Yu| Sakano, Noriko| Kubo, Masayuki| Takemoto, Kei| Masatomi, Chie| Ogino, Keiki|
Abstract We examined the relationships between dietary carbohydrate, protein, fat, and the ratio of n6/n3 fatty acid intakes with the predicted 10-year coronary heart disease (CHD) risk in a general Japanese population. We used the Framingham risk score to determine the 10-year CHD risk of the subjects, who were employees of 6 companies in a single prefecture in Japan. After excluding the subjects who reported any history of angina pectoris, myocardial infarction, diabetes, or cancer, and those with missing data resulting in the inability of estimation of 10-year CHD risk and food intakes, the final data analysis was carried out for 809 subjects. The logistic regression models revealed a significantly increased odds ratio of 10-year CHD risk in the subjects with the highest tertile of carbohydrate intake (% energy) (odds ratio 3.64, 95% CI, 2.07-6.40);after adjustment for other variables, the odds ratio for the 10-year CHD risk was also higher in the subjects with the highest tertile of carbohydrate intake (odds ratio 1.72, 95% CI, 0.70-4.25). We also found that fat intake and the ratio of n6/n3 fatty acids were inversely associated with the predicted 10-year CHD risk (p for trend<0.01). The present findings added evidence of a positive association of dietary carbohydrate and inverse associations of total fat and n6/n3 fatty acid ratio with the predicted 10-year CHD risk in a general Japanese population.
Keywords fat intake carbohydrate intake ratio of n6/n3 fatty acids 10-year coronary heart disease risk Japanese
Amo Type Original Article
Published Date 2014-06
Publication Title Acta Medica Okayama
Volume volume68
Issue issue3
Publisher Okayama University Medical School
Start Page 129
End Page 135
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2014 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 24942790
Web of Science KeyUT 000337655600001
Related Url http://ousar.lib.okayama-u.ac.jp/metadata/52516
Author Iwanaga, Suketaka| Sakano, Noriko| Taketa, Kazuhisa| Takahashi, Noriko| Wang, Da-Hong| Takahashi, Hidekazu| Kubo, Masayuki| Miyatake, Nobuyuki| Ogino, Keiki|
Published Date 2013-02-01
Publication Title Obesity Research & Clinical Practice
Content Type Journal Article
Author Inoue, Kiyomi| Sakano, Noriko| Ogino, Keiki| Sato, Yoshie| Wang, Da-Hong| Kubo, Masayuki| Takahashi, Hidekazu| Kanbara, Sakiko| Miyatake, Nobuyuki|
Published Date 2013-03-01
Publication Title Journal of Clinical Biochemistry and Nutrition
Volume volume52
Issue issue2
Content Type Journal Article