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Author Miyaji, Mary| Furuta, Ryohei| Hosoya, Osamu| Sano, Kuniaki| Hara, Norikazu| Kuwano, Ryozo| Kang, Jiyoung| Tateno, Masaru| Tsutsui, Kimiko M.| Tsutsui, Ken|
Keywords Biochemistry Molecular biology
Published Date 2020-10-29
Publication Title Scientific Reports
Volume volume10
Issue issue1
Publisher Nature Research
Start Page 18550
ISSN 2045-2322
Content Type Journal Article
language 英語
OAI-PMH Set 岡山大学
Copyright Holders © The Author(s) 2020
File Version publisher
PubMed ID 33122676
DOI 10.1038/s41598-020-75004-w
Web of Science KeyUT 000587689500013
Related Url isVersionOf https://doi.org/10.1038/s41598-020-75004-w
Author Onoda, Akihisa| Hosoya, Osamu| Sano, Kuniaki| Kiyama, Kazuko| Kimura, Hiroshi| Kawano, Shinji| Furuta, Ryohei| Miyaji, Mary| Tsutsui, Ken| Tsutsui, Kimiko M.|
Published Date 2014-07-17
Publication Title Nucleic Acids Research
Volume volume42
Issue issue14
Content Type Journal Article
Author Alamusi| Matsuo, Toshihiko| Hosoya, Osamu| Tsutsui, Kimiko M.| Uchida, Tetsuya|
Published Date 2013-09
Publication Title Journal of Artificial Organs
Volume volume16
Issue issue3
Content Type Journal Article
JaLCDOI 10.18926/AMO/31681
FullText URL fulltext.pdf
Author Iguchi, Hiroki| Watanabe, Masami| Kamitani, Akihiro| Nagai, Atsushi| Hosoya, Osamu| Tsutsui, Kimiko| Kumon, Hiromi|
Abstract <p>Dynamin is a protein essential to endocytosis. Dynamin 2, a dynamin isoform, is expressed most intensely in testicular tissue; however, precise localization has never been studied. Therefore, we investigated the expression of dynamin 2 in rat testicular tissue using immunohistochemical methods, and discuss here the physiological function of this protein. Testicular tissues were obtained from Wistar rats at 10, 21 and 63 days of age. Immunohistochemistrical examination and Western blot analysis were conducted using dynamin 2 specific antibody. Western blot analysis showed that expression in 21- and 63-day-old rats was more intense than that in 10-day-old rats. Dynamin 2 expression was observed using immunohistochemical method in the seminiferous tubules of all rats. In the 63-day-old rats, the expression was intense, especially in spermatids in the earlier maturation stages and in spermatocytes, and was observed in Sertoli cells. However, in spermatids, the expression gradually declined as spermatids matured to spermatozoa. In the 21-day-old rats, the expression was evident in spermatocytes and Sertoli cells, but that in the 10-day-old rats was weak. Intense expression of dynamin 2 during spermatogenesis suggests that this protein plays an important role in this process.</p>
Keywords dynamin 2 endocytosis spermatogenesis
Amo Type Article
Published Date 2002-08
Publication Title Acta Medica Okayama
Volume volume56
Issue issue4
Publisher Okayama University Medical School
Start Page 205
End Page 209
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 12199526
Web of Science KeyUT 000177382600006