著者 Onoda, Akihisa| Hosoya, Osamu| Sano, Kuniaki| Kiyama, Kazuko| Kimura, Hiroshi| Kawano, Shinji| Furuta, Ryohei| Miyaji, Mary| Tsutsui, Ken| Tsutsui, Kimiko M.|
発行日 2014-07-17
出版物タイトル Nucleic Acids Research
42巻
14号
資料タイプ 学術雑誌論文
著者 Alamusi| Matsuo, Toshihiko| Hosoya, Osamu| Tsutsui, Kimiko M.| Uchida, Tetsuya|
発行日 2013-09
出版物タイトル Journal of Artificial Organs
16巻
3号
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/AMO/31681
フルテキストURL fulltext.pdf
著者 Iguchi, Hiroki| Watanabe, Masami| Kamitani, Akihiro| Nagai, Atsushi| Hosoya, Osamu| Tsutsui, Kimiko| Kumon, Hiromi|
抄録 <p>Dynamin is a protein essential to endocytosis. Dynamin 2, a dynamin isoform, is expressed most intensely in testicular tissue; however, precise localization has never been studied. Therefore, we investigated the expression of dynamin 2 in rat testicular tissue using immunohistochemical methods, and discuss here the physiological function of this protein. Testicular tissues were obtained from Wistar rats at 10, 21 and 63 days of age. Immunohistochemistrical examination and Western blot analysis were conducted using dynamin 2 specific antibody. Western blot analysis showed that expression in 21- and 63-day-old rats was more intense than that in 10-day-old rats. Dynamin 2 expression was observed using immunohistochemical method in the seminiferous tubules of all rats. In the 63-day-old rats, the expression was intense, especially in spermatids in the earlier maturation stages and in spermatocytes, and was observed in Sertoli cells. However, in spermatids, the expression gradually declined as spermatids matured to spermatozoa. In the 21-day-old rats, the expression was evident in spermatocytes and Sertoli cells, but that in the 10-day-old rats was weak. Intense expression of dynamin 2 during spermatogenesis suggests that this protein plays an important role in this process.</p>
キーワード dynamin 2 endocytosis spermatogenesis
Amo Type Article
発行日 2002-08
出版物タイトル Acta Medica Okayama
56巻
4号
出版者 Okayama University Medical School
開始ページ 205
終了ページ 209
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 12199526
Web of Science KeyUT 000177382600006