ID | 57903 |
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Author |
Sakakibara, Akira
Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Kaken ID
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Takebe, So
Graduate School of Biology-Oriented Science and Technology, Kindai University
Ide, Toru
Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Hayakawa, Tohru
Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
ORCID
Kaken ID
publons
researchmap
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Abstract | Cry46Ab from Bacillus thuringiensis TK-E6 is a new mosquitocidal toxin with aerolysin-type architecture, and has been shown that co-administration of Cry46Ab with other mosquitocidal Cry toxins results in synergistic toxicity against Culex pipiens Coquillett (Diptera: Culicidae) mosquito larvae. Cry46Ab, therefore, is expected to find use in improving the insecticidal activity of B. thuringiensis-based bioinsecticides. In the present study, the mode of action of Cry46Ab was explored by single-channel measurements of Cry46Ab channel-pores. The single-channel conductances of channel-pores formed in planar lipid bilayers by Cry46Ab were determined to be 31.8 +/- 2.7 pS in 150 mM NaCl and 24.2 +/- 0.7 pS in 150 mM CaCl2. Ion-selectivity measurements revealed that the channel-pores formed by Cry46Ab were cation selective. The permeability ratio of K+ to Cl-was approximately 4, and the preferences for cations were K+ > Na+, K+ > Ca2+, and Ca2+ > Na+. A calcein release assay using liposomes suggested that Cry46Ab influences the integrity of membrane vesicles. Formation of cation-selective channel-pores has been observed with other insecticidal Cry toxins that have structures distinct from those of Cry46Ab; the capability of forming such pores may be a property required of insecticidal toxins.
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Keywords | Bacillus thuringiensis
Cry46Ab toxin
Planar lipid bilayer
Single-channel analysis
Calcein release assay
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Published Date | 2019-08-16
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Publication Title |
Applied Entomology and Zoology
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Volume | volume54
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Issue | issue4
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Publisher | Springer
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Start Page | 389
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End Page | 398
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ISSN | 0003-6862
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NCID | AA00543238
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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File Version | author
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DOI | |
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Related Url | isVersionOf https://doi.org/10.1007/s13355-019-00635-z
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License | https://creativecommons.org/licenses/by-nc-nd/4.0/
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Citation | Sakakibara, A., Takebe, S., Ide, T. et al. Characterization of the channel-pores formed by Bacillus thuringiensis Cry46Ab toxin in planar lipid bilayers. Appl Entomol Zool 54, 389–398 (2019). https://doi.org/10.1007/s13355-019-00635-z
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Funder Name |
Japan Society for the Promotion of Science
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助成番号 | JP18K05675
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