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ID 57903
著者
Sakakibara, Akira Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University Kaken ID researchmap
Takebe, So Graduate School of Biology-Oriented Science and Technology, Kindai University
Ide, Toru Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Hayakawa, Tohru Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University ORCID Kaken ID publons researchmap
抄録
Cry46Ab from Bacillus thuringiensis TK-E6 is a new mosquitocidal toxin with aerolysin-type architecture, and has been shown that co-administration of Cry46Ab with other mosquitocidal Cry toxins results in synergistic toxicity against Culex pipiens Coquillett (Diptera: Culicidae) mosquito larvae. Cry46Ab, therefore, is expected to find use in improving the insecticidal activity of B. thuringiensis-based bioinsecticides. In the present study, the mode of action of Cry46Ab was explored by single-channel measurements of Cry46Ab channel-pores. The single-channel conductances of channel-pores formed in planar lipid bilayers by Cry46Ab were determined to be 31.8 +/- 2.7 pS in 150 mM NaCl and 24.2 +/- 0.7 pS in 150 mM CaCl2. Ion-selectivity measurements revealed that the channel-pores formed by Cry46Ab were cation selective. The permeability ratio of K+ to Cl-was approximately 4, and the preferences for cations were K+ > Na+, K+ > Ca2+, and Ca2+ > Na+. A calcein release assay using liposomes suggested that Cry46Ab influences the integrity of membrane vesicles. Formation of cation-selective channel-pores has been observed with other insecticidal Cry toxins that have structures distinct from those of Cry46Ab; the capability of forming such pores may be a property required of insecticidal toxins.
キーワード
Bacillus thuringiensis
Cry46Ab toxin
Planar lipid bilayer
Single-channel analysis
Calcein release assay
備考
This fulltext will be available in Aug 2020
発行日
2019-08-16
出版物タイトル
Applied Entomology and Zoology
54巻
4号
出版者
Springer
開始ページ
389
終了ページ
398
ISSN
0003-6862
NCID
AA00543238
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
論文のバージョン
author
DOI
Web of Science KeyUT
関連URL
isVersionOf https://doi.org/10.1007/s13355-019-00635-z
ライセンス
https://creativecommons.org/licenses/by-nc-nd/4.0/
Citation
Sakakibara, A., Takebe, S., Ide, T. et al. Characterization of the channel-pores formed by Bacillus thuringiensis Cry46Ab toxin in planar lipid bilayers. Appl Entomol Zool 54, 389–398 (2019). https://doi.org/10.1007/s13355-019-00635-z
助成機関名
日本学術振興会
助成番号
JP18K05675