| FullText URL | fulltext.pdf |
|---|---|
| Author | Ogawa, Hirohito| Fujikura, Daisuke| Namba, Hikaru| Yamashita, Nobuko| Honda, Tomoyuki| Yamada, Masao| |
| Keywords | HHV-6B nectin-2 CD112 CD134 virus entry glycoprotein B |
| Published Date | 2022-01-16 |
| Publication Title | Viruses-Basel |
| Volume | volume14 |
| Issue | issue1 |
| Publisher | MDPI |
| Start Page | 160 |
| ISSN | 1999-4915 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2022 by the authors. |
| File Version | publisher |
| PubMed ID | 35062364 |
| DOI | 10.3390/v14010160 |
| Web of Science KeyUT | 000746000400001 |
| Related Url | isVersionOf https://doi.org/10.3390/v14010160 |
| FullText URL | JVMS81_8_1191.pdf |
|---|---|
| Author | Ogawa, Hirohito| Hirayama, Haruko| Tanaka, Satsuki| Yata, Norio| Namba, Hikaru| Yamashita, Nobuko| Yonemitsu, Kenzo| Maeda, Ken| Mominoki, Katsumi| Yamada, Masao| |
| Keywords | animal experimental facility domestic pig hepatitis E virus zoonosis |
| Published Date | 2019-08-24 |
| Publication Title | Journal of Veterinary Medical Science |
| Volume | volume81 |
| Issue | issue8 |
| Publisher | The Japanese Society of Veterinary Science |
| Start Page | 1191 |
| End Page | 1196 |
| ISSN | 0916-7250 |
| NCID | AA10796138 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | ©2019 The Japanese Society of Veterinary Science |
| File Version | publisher |
| PubMed ID | 31281141 |
| DOI | 10.1292/jvms.19-0086 |
| Web of Science KeyUT | 000492854900021 |
| Related Url | isVersionOf https://doi.org/10.1292/jvms.19-0086 |
| JaLCDOI | 10.18926/AMO/53674 |
|---|---|
| FullText URL | 69_5_279.pdf |
| Author | Saito, Yukie| Fujii, Yousuke| Yashiro, Masato| Tsuge, Mitsuru| Nosaka, Nobuyuki| Yamashita, Nobuko| Yamada, Mutsuko| Tsukahara, Hirokazu| Morishima, Tsuneo| |
| Abstract | Lung hyperpermeability affects the development of acute respiratory distress syndrome (ARDS), but therapeutic strategies for the control of microvascular permeability have not been established. We examined the effects of edaravone, dexamethasone, and N-monomethyl-L-arginine (L-NMMA) on permeability changes in human pulmonary microvascular endothelial cells (PMVEC) under a hypercytokinemic state. Human PMVEC were seeded in a Boyden chamber. After monolayer confluence was achieved, the culture media were replaced respectively by culture media containing edaravone, dexamethasone, and L-NMMA. After 24-h incubation, the monolayer was stimulated with tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β). Fluorescein-labeled dextran was added. Then the trans-human PMVEC leak was measured. Expressions of vascular endothelial-cadherin (VE-cadherin) and zonula occludens-1 protein (ZO-1) were evaluated using real-time quantitative polymerase chain reaction and immunofluorescence microscopy. The results showed that TNF-α＋IL-1β markedly increased pulmonary microvascular permeability. Pretreatment with edaravone, dexamethasone, or L-NMMA attenuated the hyperpermeability and inhibited the cytokine-induced reduction of VE-cadherin expression on immunofluorescence staining. Edaravone and dexamethasone increased the expression of ZO-1 at both the mRNA and protein levels. Edaravone and dexamethasone inhibited the permeability changes of human PMVEC, at least partly through an enhancement of VE-cadherin. Collectively, these results suggest a potential therapeutic approach for intervention in patients with ARDS. |
| Keywords | pulmonary microvascular endothelial cells permeability edaravone vascular endothelial-cadherin zonula occludens-1 protein |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2015-10 |
| Volume | volume69 |
| Issue | issue5 |
| Publisher | Okayama University Medical School |
| Start Page | 279 |
| End Page | 290 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | CopyrightⒸ 2015 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 26490025 |
| Web of Science KeyUT | 000365519600004 |
| Author | Yashiro, Masato| Tsukahara, Hirokazu| Matsukawa, Akihiro| Yamada, Mutsuko| Fujii, Yosuke| Nagaoka, Yoshiharu| Tsuge, Mitsuru| Yamashita, Nobuko| Ito, Toshihiro| Yamada, Masao| Masutani, Hiroshi| Yodoi, Junji| Morishima, Tsuneo| |
|---|---|
| Published Date | 2013-08-01 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume125 |
| Issue | issue2 |
| Content Type | Journal Article |
| Author | Yashiro, Masato| Tsukahara, Hirokazu| Matsukawa, Akihiro| Yamada, Mutsuko| Fujii, Yosuke| Nagaoka, Yoshiharu| Tsuge, Mitsuru| Yamashita, Nobuko| Ito, Toshihiro| Yamada, Masao| Masutani, Hiroshi| |
|---|---|
| Published Date | 2013-01 |
| Publication Title | Critical Care Medicine |
| Volume | volume41 |
| Issue | issue1 |
| Content Type | Journal Article |
| Author | Yamashita, Nobuko| |
|---|---|
| Published Date | 2012-04-01 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume124 |
| Issue | issue1 |
| Content Type | Journal Article |
| JaLCDOI | 10.18926/AMO/31961 |
|---|---|
| FullText URL | fulltext.pdf |
| Author | Yamashita, Nobuko| Kimura, Hiroshi| Morishima, Tsuneo| |
| Abstract | Epstein-Barr virus (EBV) is usually maintained in an asymptomatic and latent form by the host immune system, and primarily by EBV-specific cytotoxic T cells (CTLs). However, EBV has been linked to several refractory diseases such as EBV-associated hemophagocytic syndrome(EBV-AHS) and chronic active EBV infection (CAEBV). In these ectopic diseases, EBV infects T/NK cells, causing severe immunodeficiency with a very high EBV load. In recent years, the laboratory procedure to assess these types of EBV infections has been improved. In particular, real-time polymerase chain reaction (PCR) has been used to quantify the EBV load, and the MHC: peptide tetramer assay has been used to quantitate EBV-specific CTLs; these tests have been employed for the management of the illnesses associated with EBV infection. Here, we have reviewed the recent progress in the clinical application of these assays. The pathogenesis of EBV-infected T/NK cells, and the host immune response to infection, including the roles carried out by innate immunity and inflammatory cytokines, are likely to be revealed in the future. |
| Keywords | chronic active Epstein-Barr virus infection Epstein-Barr virus-associated hemophagocytic syndrome Real-time PCR tetramer |
| Amo Type | Review |
| Publication Title | Acta Medica Okayama |
| Published Date | 2005-12 |
| Volume | volume59 |
| Issue | issue6 |
| Publisher | Okayama University Medical School |
| Start Page | 239 |
| End Page | 246 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 16418766 |
| Web of Science KeyUT | 000234176600001 |
| Author | Yamashita, Nobuko| |
|---|---|
| Published Date | 2003-06-30 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
