ID | 67189 |
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Author |
Mao, Zhiyuan
Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences
Li, Xingyue
Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences
Li, Zhenhua
Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences
Shen, Liangliang
Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences
Li, Xiaoyi
Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences
Yang, Yanyan
Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences
Wang, Wenda
Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences
Kuang, Tingyun
Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences
Shen, Jian-Ren
Institute for Interdisciplinary Science, and Graduate School of Natural Science and Technology, Okayama University
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Han, Guangye
Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences
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Abstract | Cryptophyte algae are an evolutionarily distinct and ecologically important group of photosynthetic unicellular eukaryotes. Photosystem II (PSII) of cryptophyte algae associates with alloxanthin chlorophyll a/c-binding proteins (ACPs) to act as the peripheral light-harvesting system, whose supramolecular organization is unknown. Here, we purify the PSII-ACPII supercomplex from a cryptophyte alga Chroomonas placoidea (C. placoidea), and analyze its structure at a resolution of 2.47 & Aring; using cryo-electron microscopy. This structure reveals a dimeric organization of PSII-ACPII containing two PSII core monomers flanked by six symmetrically arranged ACPII subunits. The PSII core is conserved whereas the organization of ACPII subunits exhibits a distinct pattern, different from those observed so far in PSII of other algae and higher plants. Furthermore, we find a Chl a-binding antenna subunit, CCPII-S, which mediates interaction of ACPII with the PSII core. These results provide a structural basis for the assembly of antennas within the supercomplex and possible excitation energy transfer pathways in cryptophyte algal PSII, shedding light on the diversity of supramolecular organization of photosynthetic machinery.
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Note | The version of record of this article, first published in Nature Communications, is available online at Publisher’s website: http://dx.doi.org/10.1038/s41467-024-48878-x
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Published Date | 2024-05-28
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Publication Title |
Nature Communications
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Volume | volume15
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Issue | issue1
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Publisher | Nature Portfolio
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Start Page | 4535
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ISSN | 2041-1723
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © The Author(s) 2024
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File Version | publisher
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DOI | |
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Related Url | isVersionOf https://doi.org/10.1038/s41467-024-48878-x
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License | http://creativecommons.org/licenses/by/4.0/.
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Citation | Mao, Z., Li, X., Li, Z. et al. Structure and distinct supramolecular organization of a PSII-ACPII dimer from a cryptophyte alga Chroomonas placoidea. Nat Commun 15, 4535 (2024). https://doi.org/10.1038/s41467-024-48878-x
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Funder Name |
National Key R&D Program of China
China Postdoctoral Science Foundation
National Natural Science Foundation of China
CAS
Science & Technology Specific Project in Agricultural High-tech Industrial Demonstration Area of the Yellow River Delta
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助成番号 | 2022YFA0911900
2022YFC3401800
2020YFA0907600
2022M711490
32200199
YSBR-004
XDA26050402
JCTD-2020-06
2020081
2022SZX12
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