ID | 32971 |
FullText URL | |
Author |
Ito, Tatsuo
Morimoto, Yuki
Yoshida, Aki
Jitsumori, Yoshimi
Sonobe, Hiroshi
Inoue, Hajime
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Abstract | About 97% of synovial sarcomas harbor the SYT-SSX fusion gene by chromosomal
translocation. We found that the histone deacetylase (HDAC) inhibitor FK228
significantly suppressed the growth of synovial sarcoma cells as compared with that of
osteosarcoma. The 50% growth inhibition IC50 value we obtained for FK228 was 0.02-0.2 nM, and it indicates that its suppression effect on synovial sarcoma cells is the highest of any of the HDAC inhibitors yet reported. It was not likely that the growth suppression of FK228 depends on the doubling time of these cells. Introduction of SYT-SSX cDNA into HEK293 cells enhanced the sensitivity of the cells for FK228.
Immunostaining of the FK228-treated cells using an anti-acetyl-histone H3 antibody showed that FK228 inhibits deacetylation of histone. In a mice assay, the growth of synovial sarcoma cells was markedly inhibited by FK228 treatment, and the invasion of tumors into surrounding tissues was suppressed. These results suggest that FK228 may be useful in developing therapeutic strategies to treat synovial sarcoma.
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Keywords | histone deacetylase inhibitor
synovial sarcoma
growth inhibition
in vivo
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Note | Digital Object Identifer:10.1016/j.canlet.2004.10.030
Published with permission from the copyright holder. This is the author's copy, as published in Cancer Letters, June 2005, Volume 224, Issue 2, Pages 311-319. Publisher URL:http://dx.doi.org/10.1016/j.canlet.2004.10.030 Direct access to Thomson Web of Science record Copyright © 2004 Elsevier Ireland Ltd. All rights reserved. |
Published Date | 2005-06-28
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Publication Title |
Cancer Letters
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Volume | volume224
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Issue | issue2
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Publisher | Elsevier Ireland Ltd.
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Start Page | 311
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End Page | 319
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ISSN | 0304-3835
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NCID | AA00598513
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Content Type |
Journal Article
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language |
English
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Copyright Holders | Elsevier Ireland Ltd.
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File Version | author
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Refereed |
True
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DOI | |
PubMed ID | |
Web of Science KeyUT | |
Submission Path | biology_general/28
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