FullText URL fulltext.pdf
Author Ikeda, Risa| Kurokawa, Miki| Murai, Momoka| Saito, Noboru| Ando, Motonori|
Keywords heliozoa immunocytochemistry microtubule glutaraldehyde confocal microscopy
Published Date 2020
Publication Title Acta Protozoologica
Volume volume59
Issue issue1
Start Page 1
End Page 12
ISSN 0065-1583
NCID AA00510002
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
File Version publisher
DOI 10.4467/16890027AP.20.001.12157
Web of Science KeyUT 000605760600001
Title Alternative Cytoskeletal elements in an acoelomorph worm, Praesagittifera naikaiensis
FullText URL poalas_034_021_180706.pdf
Author Ikeda, Risa| Fujiwara, Chiho| Hamada, Mayuko| Sakamoto, Tatsuya| Saito, Noboru| Ando, Motonori|
Abstract Acoel flatworms can move in a variety of ways such as muscular and ciliary movements via cytoskeletal elements and their neural regulations. However, those locomotive mechanisms have not yet been fully elucidated. In this study, we examined the distribution of cytoskeletal elements including filamentous actin (F-actin) and tubulin, and the neuroanatomical organization in an acoelomorph worm, Praesagittifera naikaiensis (P. naikaiensis). Video microscopy revealed the elongation/contraction and the bending/rotation processes, and the ciliary gliding movement of P. naikaiensis. Histochemical and morphological analysis demonstrated that F-actin networks of inner longitudinal and outer circular muscle fibers were positioned along the entire surface of the body, and that the average distance between the circular muscle fibers in the contracted organism was decreased in the anterior region compared with that in the elongated organism. Electron microscopy showed dense bodies on the muscle cells of P. naikaiensis, which indicates that those muscle cells have the appearance of vertebrate smooth muscle cells. Immunohistochemical analysis revealed that -tubulin-positive signals on the ciliary microtubules had close contact with the F-actin network, and that neurite bundles labelled with anti dSap47 antibody as a neuronal marker run along the anterior-posterior body axis. These results indicate that the well-organized cytoskeletal elements and their neural control systems are preserved in P. naikaiensis, and that their mechanisms involved in those regulation systems are similar to those vertebrate systems. Further studies are needed to clarify the physiological mechanisms underlying the muscular and ciliary movements in P. naikaiensis.
Publication Title Proceedings of Okayama Association for Laboratory Animal Science
Published Date 2018-04
Volume volume34
Start Page 21
End Page 27
language Japanese
File Version publisher
NAID 120006469118
Title Alternative Structure and function of tegmentum vasculosum in avian cochlea
FullText URL poalas_033_026_030.pdf
Author Ikeda, Risa| Otono, Tsuyoshi| Ikeda, Naoya| Saito, Noboru| Ando, Motonori|
Abstract In spite of the importance of endocochlear DC potential (EP) and the K+-rich endolymph for the avian cochlea, the structure and function of the tegmentum vasculosum (TV) has not yet been fully elucidated, compared with those of the stria vascularis in the mammalian cochlea. In this study, we examined structural analysis of the epithelial cells, gene expressions of Na+-K+-ATPase (Atp1A1) and Kir4.1 (Kcnj10), and protein localizations of Na+-K+-ATPase and Kir4.1, in the TV. Tight junctional structures were observed between epithelial dark cells and light cells in the TV. Both Atp1a1 and Kcnj10 genes were detected in the TV. In addition, immunopositive signals for both Na+-K+-ATPase and Kir4.1 were recognized in the TV. These results indicate that Na+-K+-ATPase and Kir4.1 play roles in maintaining the EP and high K+ concentration of the endolymph. Further studies are needed to clarify the physiological functions of the TV. This is the first report which demonstrates that gene and protein expression data contribute to the avian inner ear homeostasis.
Publication Title Proceedings of Okayama Association for Laboratory Animal Science
Published Date 2017-04
Volume volume33
Start Page 26
End Page 30
language Japanese
File Version publisher
Author Yamaji, Mayumi| Inoue, Risa| Edamatsu, Midori| Ando, Motonori|
Published Date 2015-04
Publication Title 岡山実験動物研究会報
Volume volume31
Content Type Journal Article
JaLCDOI 10.18926/15108
Title Alternative The New Type of Lectures on the Development of Science Teaching Plans and Materials Tried in the Department ofEducation
FullText URL 009_097_102.pdf
Author Inada, Yoshihiko| Irie, Izumi| Atobe, Kozo| Ando, Motonori| Ishikawa, Teruhiko| Uno, Koji| Ogura, Hisakazu| Kakihara, Seiji| Kato, Kuranoshin| Kita, Masakazu| Tanaka, Kenji|
Abstract 岡山大学教育学部の理科教育講座に所属する3年生を対象に,理科教材・授業案開発を指向した新しい講義を始めたのでその報告を行う。本講義では,近隣の小,中学校や公民館,科学館等の協力も得て実践の場を確保し,開発した教材や授業案を実践することで生きた教材開発の訓練を行なうことを目指している。教科内容学担当教員と教科教育学担当教員の協働を模索すると同時に,大学教員の教科内容の専門知識や技量を教材や授業案に有効に活用できる講義を意識した。
Keywords 学部講義 教材授業案開発 (Development of Teaching Plans and Teaching Materials) 授業実践 (Education practice) 理科教育 (Science Education Class) 新カリキュラム (New Curriculum)
Publication Title 岡山大学教育実践総合センター紀要
Published Date 2009-03-10
Volume volume9
Issue issue1
Start Page 97
End Page 102
ISSN 1346-3705
language Japanese
File Version publisher
NAID 120002307783
JaLCDOI 10.18926/14367
Title Alternative The Lectures on the Development of Teaching Plans and Teaching Materials Tried in the Science Education Class in Master's Course, Okayama University
FullText URL 007_001_007.pdf
Author Inada, Yoshihiko| Kakihara, Seiji| Kita, Masakazu| Yamashita, Nobuhiko| Ishikawa, Teruhiko| Ogura, Hisakazu| Ando, Motonori| Kusachi, Isao| Kato, Kuranoshin| Yoshioka, Tsutomu| Kawata, Yuki| Hirakawa, Motoshi| Hashimoto, Seiji| Akazaki, Tetsuya| Higashi, Nobuhiko| Becchaku, Akio|
Abstract 岡山大学大学院教育学研究科の理科教育専攻の講義として、教材開発・授業案開発をテーマとした新しい講義を試みたのでその報告を行う。本講義は、理科教育講座に所属する大学院生、理科教育講座の大学教員、附属学校の理科関係の教諭が三者協働で進めることが特徴である。課題設定、教材開発を含めた実践準備および実践を院生チームを組み遂行させ、さらに経験や専門性の異なる人材と論議を重ねて活動を進めることを通じ、将来、協働で学校現場の課題提案・解決を行うことのできる能力を養うことを目標とした。
Keywords 大学院講義 (Science Education Class in the Master's Course) 附属学校 (Attached School) 授業実践 (education practice) 教材開発 (Development of Teaching Materials) 授業案開発 (Development of Teaching Plans)
Publication Title 岡山大学教育実践総合センター紀要
Published Date 2007-03-10
Volume volume7
Issue issue1
Start Page 1
End Page 7
ISSN 1346-3705
language Japanese
File Version publisher
NAID 120002308464