ID | 66922 |
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Ishida, Hikaru
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Yasui, Norihisa
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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Yamashita, Atsuko
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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Abstract | Taste receptor type 1 (T1r) proteins are responsible for recognizing nutrient chemicals in foods. In humans, T1r2/T1r3 and T1r1/T1r3 heterodimers serve as the sweet and umami receptors that recognize sugars or amino acids and nucleotides, respectively. T1rs are conserved among vertebrates, and T1r2a/T1r3 from medaka fish is currently the only member for which the structure of the ligand-binding domain (LBD) has been solved. T1r2a/T1r3 is an amino acid receptor that recognizes various l-amino acids in its LBD as observed with other T1rs exhibiting broad substrate specificities. Nevertheless, the range of chemicals that are recognized by T1r2a/T1r3LBD has not been extensively explored. In the present study, the binding of various chemicals to medaka T1r2a/T1r3LBD was analyzed. A binding assay for amino acid derivatives verified the specificity of this protein to l-alpha-amino acids and the importance of alpha-amino and carboxy groups for receptor recognition. The results further indicated the significance of the alpha-hydrogen for recognition as replacing it with a methyl group resulted in a substantially decreased affinity. The binding ability to the protein was not limited to proteinogenic amino acids, but also to non-proteinogenic amino acids, such as metabolic intermediates. Besides l-alpha-amino acids, no other chemicals showed significant binding to the protein. These results indicate that all of the common structural groups of alpha-amino acids and their geometry in the l-configuration are recognized by the protein, whereas a wide variety of alpha-substituents can be accommodated in the ligand binding sites of the LBDs.
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Published Date | 2024-03-22
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Publication Title |
PLoS ONE
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Volume | volume19
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Issue | issue3
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Publisher | Public Library of Science
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Start Page | e0300981
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ISSN | 1932-6203
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © 2024 Ishida et al.
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File Version | publisher
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Related Url | isVersionOf https://doi.org/10.1371/journal.pone.0300981
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License | https://creativecommons.org/licenses/by/4.0/
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Citation | Article Source: Chemical range recognized by the ligand-binding domain in a representative amino acid-sensing taste receptor, T1r2a/T1r3, from medaka fish Ishida H, Yasui N, Yamashita A (2024) Chemical range recognized by the ligand-binding domain in a representative amino acid-sensing taste receptor, T1r2a/T1r3, from medaka fish. PLOS ONE 19(3): e0300981. https://doi.org/10.1371/journal.pone.0300981
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Funder Name |
Japan Society for the Promotion of Science
Takeda Science Foundation
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助成番号 | JP20H03195
JP20H04778
JP21H05524
JP23H02424
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