JaLCDOI 10.18926/AMO/30883
FullText URL fulltext.pdf
Author Tomochika, Hiroshi| Gouchi, Akira| Okanobu, Kouji| Sasaki, Akinori| Fuchimoto, Sadanori| Orita, Kunzo|
Abstract <p>In order to improve the postoperative prognosis of gastric cancer patients we have performed preoperative endoscopic intratumoral administration of various biological response modifiers. In the present study we have investigated the kinetics and the immune response augmenting effect of intratumorally injected PSK, a protein-bound polysaccharide preparation, by immunohistochemical methods using anti-PSK antibody and various other antibodies. PSK-containing cells were located in the tumor tissues and follicular marginal zones of regional lymph nodes. Intratumorally administered PSK appeared to be phagocytized by the histiocytes and to cause them to become antigen-presenting cells. These cells may play a major role in augmenting immune responses in gastric cancer patients.</p>
Keywords PSK immunohistochemistry gastric cancer
Amo Type Article
Published Date 1989-10
Publication Title Acta Medica Okayama
Volume volume43
Issue issue5
Publisher Okayama University Medical School
Start Page 289
End Page 297
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2610006
Web of Sience KeyUT A1989CA06200005
JaLCDOI 10.18926/AMO/30882
FullText URL fulltext.pdf
Author Maki, Yoshio| Irie, Shin| Ohashi, Teruhisa| Ohmori, Hiroyuki|
Abstract <p>We report a case of unilateral hyperplasia of the adrenal medulla. The patient showed clinical features suggestive of pheochromocytoma. Removal of the hyperplastic adrenal gland resulted in complete disappearance of all prior symptoms, decrease of the plasma and urinary catecolamine levels and no high uptake in [133I] metaiodobenzylguanidine scintigraphy. A histological study revealed diffuse hyperplasia of the adrenal medulla. Up to now, there are relatively few reports of adrenal medullary hyperplasia in English literatures.</p>
Keywords adrenal medullary hyperplasia pheochromocytoma ?<sup> 131</sup> I ?metaiodobenzylguanidine scintigraphy
Amo Type Article
Published Date 1989-10
Publication Title Acta Medica Okayama
Volume volume43
Issue issue5
Publisher Okayama University Medical School
Start Page 311
End Page 315
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2610007
Web of Science KeyUT A1989CA06200008
JaLCDOI 10.18926/AMO/30881
FullText URL fulltext.pdf
Author Masuoka, Noriyoshi| Ubuka, Toshihiko| Akagi, Reiko| Yao, Kenzaburoh| Ishino, Kazushi|
Abstract <p>A new volatile derivative of taurine, N-isobutoxycarbonyltaurine methyl ester (methyl 2-(N-isobutoxycarbonylamino)ethanesulfonate), was prepared by a three-step procedure for the gas chromatographic determination of taurine in urine. First, taurine was converted to its silver salt by reaction with silver oxide; next the silver salt was reacted with isobutyl chloroformate to form the N-isobutoxycarbonyl derivative, and finally the derivative was reacted with methyl iodide to form N-isobutoxycarbonyltaurine methyl ester. The volatile derivative was analyzed by gas chromatography using a column of 3% OV-101 on Chromosorb W. When methyl 3-(N-isobutoxycarbonylamino) propanesulfonate was used as an internal standard, the calibration curve was linear between 0.5 and 5.0 mumol of taurine/ml and showed a good reproducibility. This method was applied to the determination of taurine in human urine. Recovery was 98.6 +/- 5.2%, when 1.25 to 5.0 mumol/ml of taurine was added to human urine.</p>
Keywords taurine gas chromatography taurine determination methyI 2-(N-isobutoxycarbonylamino) ethanesulfoate taurine excretion
Amo Type Article
Published Date 1989-10
Publication Title Acta Medica Okayama
Volume volume43
Issue issue5
Publisher Okayama University Medical School
Start Page 253
End Page 259
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2610003
Web of Science KeyUT A1989CA06200001
JaLCDOI 10.18926/AMO/30880
FullText URL fulltext.pdf
Author Yoshida, Shigeko| Akagi, Rriko| Ubuka, Toshihiko|
Abstract <p>Excretion of sulfate and taurine, two major metabolites of sulfur, was examined in rats to study the nutritional status of sulfur metabolism in the mammals. Rats maintained on a conventional laboratory diet excreted 1.83 +/- 0.14 mmol of free sulfate and 229.0 +/- 75.3 mumol of taurine/kg of body weight per day. When the diet was changed to a synthetic 25% casein diet, the taurine excretion decreased to 15% of the previous daily excretion, but sulfate excretion decreased only slightly. These decreased levels returned to the original levels when 5 mmol of L-cysteine/kg of body weight was administered into the stomach through a catheter. One week after the first L-cysteine administration, when sulfate and taurine excretion had returned to the original levels, 5 mmol of L-cysteine/kg of body weight was administered likewise. The rats excreted sulfur corresponding to about 95% of L-cysteine administered in the form of free sulfate and taurine within a few days following L-cysteine administration, and sulfate excretion was 3.5 times more than taurine excretion. These results seem to suggest that, in rats, sulfur metabolism is in a state of equilibrium and that sulfate is formed preferentially to taurine.</p>
Keywords sulfate taurine cysteine sulfur metabolism
Amo Type Article
Published Date 1989-10
Publication Title Acta Medica Okayama
Volume volume43
Issue issue5
Publisher Okayama University Medical School
Start Page 281
End Page 288
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2610005
Web of Science KeyUT A1989CA06200004
JaLCDOI 10.18926/AMO/30879
FullText URL fulltext.pdf
Author Horie, Yasushi| Motoi, Makoto| Ogawa, Katsuo|
Abstract <p>In order to clarify the origin of JC virus-induced brain tumors in rats, the development of tumors was sequentially analyzed histologically and immunohistochemically. Twenty-two of 30 rats (73%), which were intracerebrally inoculated with JC virus within 24 h of birth (group 1), developed, as a group, 45 brain tumors after 12 to 26 weeks. Seventeen of 27 rats (63%), which were inoculated on the 7th day after birth (group 2), developed 37 brain tumors as a group after a time 12 to 40 weeks. The tumors were found exclusively in the cerebrum. The microtumors, which were defined as tumors less than 2 mm in diameter, were located in the subependymal plate around the ventricular system. The microtumors and most part of the macrotumors consisted of cells of undifferentiated neuroectodermal nature, showing nuclear palisades and Homer-Wright-pseudorosette-like structures. Some tumor cells of macrotumors had an astrocytic nature and were positive for glial fibrillary acidic protein, S-100, Leu 7, and vimentin. In conclusion, the target cells of JC virus in rats may be undifferentiated subependymal cells of the cerebrum. The tumor cells show partial glial differentiation as they grow.</p>
Keywords JC virus brain tumor cell differentiation immunohistochemistry
Amo Type Article
Published Date 1989-10
Publication Title Acta Medica Okayama
Volume volume43
Issue issue5
Publisher Okayama University Medical School
Start Page 271
End Page 279
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2558501
Web of Science KeyUT A1989CA06200003
JaLCDOI 10.18926/AMO/30878
FullText URL fulltext.pdf
Author Edahiro, Toru| Ohta, Nobuo| Ishii, Akira|
Abstract <p>To analyze the possible major T cell recognition site(s) of chironomid antigens, we established human T cell lines and clones (CD3+ 4+ 8-) reactive to soluble extracts of the adult midge of Tokunagayusurika akamusi (TAA) and/or Chironomus yoshimatsui (CYA). All T cell lines and clones proliferated heavily in response to relatively large molecular weight fractions of TAA (MW greater than or equal to 15,000). Nine clones reactive to TAA were classified into 3 groups according to reactivity, indicating the existence of at least 3 distinct T cell recognition sites in TAA. Five T cell clones responded to both TAA and CYA, although the two chironomid antigens were serologically distinct. We conclude that T cell recognition sites of chironomid antigens are different from B cell recognition sites in humans.</p>
Keywords allergen analysis bronchial asthma chironomid lymphocyte
Amo Type Article
Published Date 1989-10
Publication Title Acta Medica Okayama
Volume volume43
Issue issue5
Publisher Okayama University Medical School
Start Page 261
End Page 270
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2610004
Web of Sience KeyUT A1989CA06200002
JaLCDOI 10.18926/AMO/30877
FullText URL fulltext.pdf
Author Ogura, Hajime| Fujiwara, Tazuko|
Abstract <p>The rescue of infectious virus from nonproducer BH RSV(-) cells by chick cellular DNA was attempted in order to investigate the functional state of endogenous and exogenous retroviral genes integrated within the cellular DNA. No infectious virus was rescued by transfection with DNAs of chick helper factor (chf)-negative chick embryo cells (CEC), chf-positive CEC or uninfected CEC producing endogenous Rous associated virus (RAV-0). On the other hand, infectious Rous viruses with the phenotype of RAV-0 and RAV-1 were rescued by transfection with DNAs of CEC which had been infected with RAV-0 and RAV-1. From these results, it seems that exogenous retroviral genes integrated in the cellular DNA are expressed rather easily by transfection while those present endogenously are not.</p>
Keywords transfection chick DNA nonproducer Rous cell virus rescue
Amo Type Article
Published Date 1989-10
Publication Title Acta Medica Okayama
Volume volume43
Issue issue5
Publisher Okayama University Medical School
Start Page 307
End Page 309
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2558502
Web of Sience KeyUT A1989CA06200007
JaLCDOI 10.18926/AMO/30876
FullText URL fulltext.pdf
Author Jitsunari, Fumihiko| Asakawa, Fumiyuki| Nakajima, Taichi| Shimada, Junko| Ogata, Masana|
Abstract <p>Chlorpyrifos, an organophosphorus insecticide, has been used to control termites since regulatory measures against the use of chlordanes were taken in September, 1986. We developed an improved gas chromatographic (GC) method for the assay of 3,5,6-trichloro-2-pyridinol (TCP) in the urine to use in the biological monitoring of exposure to chlorpyrifos. Urinary TCP was separated and determined accurately (C.V., 4%) with high sensitivity (detection limit, 10 ng/ml) and recovery (recovery greater than 90%) using a wide bore capillary column (WBC column). The accuracy and precision of the present GC method are satisfactory. The time course of urinary excretion of TCP was followed in workers. The urinary TCP level was low in the off-season and high in the busy season. Variation in the urinary TCP level corresponded to the termite control season and the length of the working period. The urinary TCP level showed a change reciprocal to the variations in the plasma cholinesterase activity. From these results, it is surmised that the urinary TCP level represents the extent of exposure to chlorpyrifos. The decrease in the level of cholinesterase activity is suggested to be due to exposure to chlorpyrifos. Determination of the urinary TCP level by GC using a WBC column is useful in the biological monitoring of chlorpyrifos in termite control workers and potentially has practical application to health care.</p>
Keywords termite control worker chlorpyrifos urinary 3 5 6-trichloro-2-pyridinol biological exposure index biological monitoring
Amo Type Article
Published Date 1989-10
Publication Title Acta Medica Okayama
Volume volume43
Issue issue5
Publisher Okayama University Medical School
Start Page 299
End Page 306
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2481940
Web of Sience KeyUT A1989CA06200006