ID | 30881 |
JaLCDOI | |
FullText URL | |
Author |
Masuoka, Noriyoshi
Ubuka, Toshihiko
Akagi, Reiko
Yao, Kenzaburoh
Ishino, Kazushi
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Abstract | A new volatile derivative of taurine, N-isobutoxycarbonyltaurine methyl ester (methyl 2-(N-isobutoxycarbonylamino)ethanesulfonate), was prepared by a three-step procedure for the gas chromatographic determination of taurine in urine. First, taurine was converted to its silver salt by reaction with silver oxide; next the silver salt was reacted with isobutyl chloroformate to form the N-isobutoxycarbonyl derivative, and finally the derivative was reacted with methyl iodide to form N-isobutoxycarbonyltaurine methyl ester. The volatile derivative was analyzed by gas chromatography using a column of 3% OV-101 on Chromosorb W. When methyl 3-(N-isobutoxycarbonylamino) propanesulfonate was used as an internal standard, the calibration curve was linear between 0.5 and 5.0 mumol of taurine/ml and showed a good reproducibility. This method was applied to the determination of taurine in human urine. Recovery was 98.6 +/- 5.2%, when 1.25 to 5.0 mumol/ml of taurine was added to human urine. |
Keywords | taurine
gas chromatography
taurine determination
methyI 2-(N-isobutoxycarbonylamino) ethanesulfoate
taurine excretion
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Amo Type | Article
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Publication Title |
Acta Medica Okayama
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Published Date | 1989-10
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Volume | volume43
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Issue | issue5
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Publisher | Okayama University Medical School
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Start Page | 253
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End Page | 259
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ISSN | 0386-300X
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NCID | AA00508441
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Content Type |
Journal Article
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language |
English
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File Version | publisher
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Refereed |
True
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PubMed ID | |
Web of Science KeyUT |