ID | 30560 |
JaLCDOI | |
FullText URL | |
Author |
Tsuji, Takao
Inoue, Junichi
Shinohara, Toru
Tsuchiya, Masao
Onoue, Kimiaki
Araki, Kiyonori
Nagashima, Hideo
Nakashima, Akira
Naito, Kunihiko
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Abstract | Fragmented sheep red blood cell (SRBC) membrane was used for detection of T-cells in liver biopsy specimens from patients with chronic active hepatitis. SRBC was separated with Lymphoprep, sonicated, then filtered through a 3 mu Millipore-membrane as a fragmented SRBC reagent. Tissue T-cells were stained by an indirect immunofluorescent technique using SRBC reagent and fluorescein isothiocyanate (FITC)-labelled rabbit anti-SRBC. Positively staining lymphocytes were present in portal tracts and in areas of piecemeal necrosis. There also seemed to be a positive correlation between the number of positively staining lymphocytes and the activity of chronic hepatitis; numerous lymphocytes being stained in areas of severe piecemeal necrosis. Our findings suggest that the fragmented SRBC technique for detection of T-cells is reliable and reproducible, that it could be used as a clinical routine method, and that it is useful for further elucidating the nature of host immune reactions on tissue levels.<
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Keywords | T-lymphocyte
T-cell
lymphocyte receptor
chronic active hepatitis
immune response.
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Amo Type | Article
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Publication Title |
Acta Medica Okayama
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Published Date | 1980-02
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Volume | volume34
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Issue | issue1
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Publisher | Okayama University Medical School
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Start Page | 51
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End Page | 59
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ISSN | 0386-300X
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NCID | AA00508441
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Content Type |
Journal Article
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language |
English
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File Version | publisher
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Refereed |
True
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PubMed ID | |
Web of Science KeyUT |