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ID 32988
FullText URL
Author
Kanai, Takahiro
Kajimoto, Taketoshi
Abstract

A monoclonal antibody (IgG) that recognizes a 53-kDa zebrafishnext brain protein was isolated and used to characterize the distribution of this protein in zebrafish.next (1) The antigen was found only in the brain and not in any other tissues such as muscle, dermis and cartilage. Within the brain, the antibody recognized extracellular matrix (ECM) outside neuronal cells. (2) Digestion by hyaluronidase released the antigen from brain tissue, and the monoclonal antibody staining was also decreased by the digestion by hyaluronidase. (3) The pattern of antigen distribution is not perineuronal, as the density of the antigen at the periphery of the cells was practically identical to that of the empty intercellular spaces. Therefore, this monoclonal antibody does not recognize the perineuronal glycocortex. (4) The antigen is distributed only in limited areas of the brain, namely in the periphery of the forebrain, the hypothalamus, the optic tectum, the interpeduncular nucleus, the cerebellum and the ventricular rim of the medulla. In the optic tectum, the antibody strongly stained the most superficial layer, and in the cerebellum, it stained the molecular but not the granular layer. These patterns of distribution are very different from those of other typical brain ECM proteins and suggest that this protein may play quite distinct roles in brain development and maintenance.

Keywords
Zebrafish
Extracellular matrix
Monoclonal antibody
Brain-specific ECM
Note
Digital Object Identifer:10.1016/j.brainres.2006.09.037
Published with permission from the copyright holder. This is the author's copy, as published in Brain Research, 19 January 2007, Volume 1129, Issue 1, Pages 53-62.
Publisher URL:http://dx.doi.org/10.1016/j.brainres.2006.09.037
Copyright © 2006 Elsevier Science B.V. Limited. All rights reserved.
Published Date
2007-01
Publication Title
Brain Research
Volume
volume1129
Issue
issue1
Start Page
53
End Page
62
Content Type
Journal Article
language
英語
Refereed
True
DOI
Submission Path
biology_general/21