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ID 32988
フルテキストURL
著者
Kanai, Takahiro Okayama University
Kajimoto, Taketoshi Okayama University
Nakayasu, Hiroshi Okayama University
抄録

A monoclonal antibody (IgG) that recognizes a 53-kDa zebrafishnext brain protein was isolated and used to characterize the distribution of this protein in zebrafish.next (1) The antigen was found only in the brain and not in any other tissues such as muscle, dermis and cartilage. Within the brain, the antibody recognized extracellular matrix (ECM) outside neuronal cells. (2) Digestion by hyaluronidase released the antigen from brain tissue, and the monoclonal antibody staining was also decreased by the digestion by hyaluronidase. (3) The pattern of antigen distribution is not perineuronal, as the density of the antigen at the periphery of the cells was practically identical to that of the empty intercellular spaces. Therefore, this monoclonal antibody does not recognize the perineuronal glycocortex. (4) The antigen is distributed only in limited areas of the brain, namely in the periphery of the forebrain, the hypothalamus, the optic tectum, the interpeduncular nucleus, the cerebellum and the ventricular rim of the medulla. In the optic tectum, the antibody strongly stained the most superficial layer, and in the cerebellum, it stained the molecular but not the granular layer. These patterns of distribution are very different from those of other typical brain ECM proteins and suggest that this protein may play quite distinct roles in brain development and maintenance.

キーワード
Zebrafish
Extracellular matrix
Monoclonal antibody
Brain-specific ECM
備考
Digital Object Identifer:10.1016/j.brainres.2006.09.037
Published with permission from the copyright holder. This is the author's copy, as published in Brain Research, 19 January 2007, Volume 1129, Issue 1, Pages 53-62.
Publisher URL:http://dx.doi.org/10.1016/j.brainres.2006.09.037
Copyright © 2006 Elsevier Science B.V. Limited. All rights reserved.
発行日
2007-01
出版物タイトル
Brain Research
1129巻
1号
開始ページ
53
終了ページ
62
資料タイプ
学術雑誌論文
言語
English
査読
有り
DOI
Submission Path
biology_general/21