ID | 53905 |
FullText URL | |
Author |
Sato, Takamaro
Yamaguchi, Tomoko
Meguro, Michio
Maeda, Hiroshi
Tada, Joji
Otani, Takayuki
Seno, Masaharu
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Abstract | [Background] : Eosinophil cationic protein (ECP) was reported previously to be involved in allergic inflammation with cytotoxic activity. On the other hand, recent studies showed that ECP did not induce cell death but inhibited the growth of cancer-derived cells. Our previous study indicated that human ECP enhanced differentiation of rat neonatal cardiomyocytes and stress fiber formation in Balb/c 3T3 mouse fibroblasts, while the effects of human ECP on human fibroblasts are unknown.
[Objective] : The present study was performed to determine the effects of human ECP on cytokine expression in human fibroblasts by protein array.
[Methods] : The effects of recombinant human ECP (rhECP) on normal human dermal fibroblasts (NHDF) were examined by assaying cell growth. Furthermore, cytokine expression of NHDF stimulated by ECP, which could influence cell growth, was evaluated by protein array.
[Results] : ECP was not cytotoxic but enhanced the growth of NHDF. The peak rhECP concentration that enhanced the cell counts by 1.56-fold was 100 ng/mL, which was significantly different from cultures without ECP stimulation (ANOVA/Scheffe’s test, P < 0.05). Array analyses indicated that ciliary neurotrophic factor (CNTF), neutrophilactivating peptide (NAP)-2, and neurotrophin (NT)-3 were significantly upregulated in NHDF stimulated with 100 ng/mL ECP compared to those without stimulation.
[Conclusion] : ECP is not cytotoxic but enhances the growth of NHDF. CNTF, NAP-2, and NT-3 were suggested to be involved in enhancing the growth of NHDF. These findings will contribute to determination of the role of ECP in allergic inflammation. (Asian Pac J Allergy Immunol 2013;31:271-6)
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Keywords | cytokine
eosinophil cationic protein
fibroblast
growth
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Note | This article is permitted to archive for OUSAR by Asian Pacific Journal of Allergy and Immunology.
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Published Date | 2013-12
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Publication Title |
Asian Pacific Journal of Allergy And Immunology
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Volume | volume31
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Issue | issue4
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Publisher | the Allergy and Immunology Society of Thailand
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Start Page | 271
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End Page | 276
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ISSN | 0125-877X
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NCID | AA10706124
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Content Type |
Journal Article
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Official Url | http://thailand.digitaljournals.org/index.php/APJAI/
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language |
English
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Copyright Holders | Asian Pacific journal of allergy and immunology
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File Version | publisher
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Refereed |
True
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DOI | |
PubMed ID | |
Web of Science KeyUT |