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ID 53905
フルテキストURL
著者
Sato, Takamaro Department of Pathophysiology - Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Soga, Yoshihiko Division of Hospital Dentistry, Central Clinical Department, Okayama University Hospital ORCID Kaken ID publons researchmap
Yamaguchi, Tomoko Department of Pathophysiology - Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Meguro, Michio Department of Pathophysiology - Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Maeda, Hiroshi Department of Pathophysiology - Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Tada, Joji Division of Dermatology, National Sanatorium Nagashima-Aiseien
Otani, Takayuki Department of Medical and Bioengineering Science, Okayama University Graduate School of Natural Science and Technology
Seno, Masaharu Department of Medical and Bioengineering Science, Okayama University Graduate School of Natural Science and Technology
Takashiba, Shogo Department of Pathophysiology - Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences ORCID Kaken ID publons researchmap
抄録
[Background] : Eosinophil cationic protein (ECP) was reported previously to be involved in allergic inflammation with cytotoxic activity. On the other hand, recent studies showed that ECP did not induce cell death but inhibited the growth of cancer-derived cells. Our previous study indicated that human ECP enhanced differentiation of rat neonatal cardiomyocytes and stress fiber formation in Balb/c 3T3 mouse fibroblasts, while the effects of human ECP on human fibroblasts are unknown. [Objective] : The present study was performed to determine the effects of human ECP on cytokine expression in human fibroblasts by protein array. [Methods] : The effects of recombinant human ECP (rhECP) on normal human dermal fibroblasts (NHDF) were examined by assaying cell growth. Furthermore, cytokine expression of NHDF stimulated by ECP, which could influence cell growth, was evaluated by protein array. [Results] : ECP was not cytotoxic but enhanced the growth of NHDF. The peak rhECP concentration that enhanced the cell counts by 1.56-fold was 100 ng/mL, which was significantly different from cultures without ECP stimulation (ANOVA/Scheffe’s test, P < 0.05). Array analyses indicated that ciliary neurotrophic factor (CNTF), neutrophilactivating peptide (NAP)-2, and neurotrophin (NT)-3 were significantly upregulated in NHDF stimulated with 100 ng/mL ECP compared to those without stimulation. [Conclusion] : ECP is not cytotoxic but enhances the growth of NHDF. CNTF, NAP-2, and NT-3 were suggested to be involved in enhancing the growth of NHDF. These findings will contribute to determination of the role of ECP in allergic inflammation. (Asian Pac J Allergy Immunol 2013;31:271-6)
キーワード
cytokine
eosinophil cationic protein
fibroblast
growth
備考
This article is permitted to archive for OUSAR by Asian Pacific Journal of Allergy and Immunology.
発行日
2013-12
出版物タイトル
Asian Pacific Journal of Allergy And Immunology
31巻
4号
出版者
the Allergy and Immunology Society of Thailand
開始ページ
271
終了ページ
276
ISSN
0125-877X
NCID
AA10706124
資料タイプ
学術雑誌論文
オフィシャル URL
http://thailand.digitaljournals.org/index.php/APJAI/
言語
英語
著作権者
Asian Pacific journal of allergy and immunology
論文のバージョン
publisher
査読
有り
DOI
PubMed ID
Web of Science KeyUT