ID | 53905 |
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著者 |
Sato, Takamaro
Department of Pathophysiology - Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Soga, Yoshihiko
Division of Hospital Dentistry, Central Clinical Department, Okayama University Hospital
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Yamaguchi, Tomoko
Department of Pathophysiology - Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Meguro, Michio
Department of Pathophysiology - Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Maeda, Hiroshi
Department of Pathophysiology - Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Tada, Joji
Division of Dermatology, National Sanatorium Nagashima-Aiseien
Otani, Takayuki
Department of Medical and Bioengineering Science, Okayama University Graduate School of Natural Science and Technology
Seno, Masaharu
Department of Medical and Bioengineering Science, Okayama University Graduate School of Natural Science and Technology
Takashiba, Shogo
Department of Pathophysiology - Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
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抄録 | [Background] : Eosinophil cationic protein (ECP) was reported previously to be involved in allergic inflammation with cytotoxic activity. On the other hand, recent studies showed that ECP did not induce cell death but inhibited the growth of cancer-derived cells. Our previous study indicated that human ECP enhanced differentiation of rat neonatal cardiomyocytes and stress fiber formation in Balb/c 3T3 mouse fibroblasts, while the effects of human ECP on human fibroblasts are unknown.
[Objective] : The present study was performed to determine the effects of human ECP on cytokine expression in human fibroblasts by protein array.
[Methods] : The effects of recombinant human ECP (rhECP) on normal human dermal fibroblasts (NHDF) were examined by assaying cell growth. Furthermore, cytokine expression of NHDF stimulated by ECP, which could influence cell growth, was evaluated by protein array.
[Results] : ECP was not cytotoxic but enhanced the growth of NHDF. The peak rhECP concentration that enhanced the cell counts by 1.56-fold was 100 ng/mL, which was significantly different from cultures without ECP stimulation (ANOVA/Scheffe’s test, P < 0.05). Array analyses indicated that ciliary neurotrophic factor (CNTF), neutrophilactivating peptide (NAP)-2, and neurotrophin (NT)-3 were significantly upregulated in NHDF stimulated with 100 ng/mL ECP compared to those without stimulation.
[Conclusion] : ECP is not cytotoxic but enhances the growth of NHDF. CNTF, NAP-2, and NT-3 were suggested to be involved in enhancing the growth of NHDF. These findings will contribute to determination of the role of ECP in allergic inflammation. (Asian Pac J Allergy Immunol 2013;31:271-6)
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キーワード | cytokine
eosinophil cationic protein
fibroblast
growth
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備考 | This article is permitted to archive for OUSAR by Asian Pacific Journal of Allergy and Immunology.
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発行日 | 2013-12
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出版物タイトル |
Asian Pacific Journal of Allergy And Immunology
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巻 | 31巻
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号 | 4号
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出版者 | the Allergy and Immunology Society of Thailand
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開始ページ | 271
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終了ページ | 276
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ISSN | 0125-877X
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NCID | AA10706124
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資料タイプ |
学術雑誌論文
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オフィシャル URL | http://thailand.digitaljournals.org/index.php/APJAI/
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言語 |
英語
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著作権者 | Asian Pacific journal of allergy and immunology
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論文のバージョン | publisher
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査読 |
有り
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