JaLCDOI 10.18926/AMO/30662
フルテキストURL fulltext.pdf
著者 Sasaki, Junzo| Imanaka, Masaaki| Watanabe, Sadahiro| Mori, Masaharu| Nakamoto, Shu| Kirizuka, Keizi| Otsuka, Nagayasu|
キーワード cytochalasin B Ehrlich ascites tumor cells cappong zeiosis cationized ferritin
Amo Type Brief Note
出版物タイトル Acta Medica Okayama
発行日 1982-08
36巻
4号
出版者 Okayama University Medical School
開始ページ 307
終了ページ 312
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 6890303
Web of Science KeyUT A1982PE61100008
JaLCDOI 10.18926/AMO/30661
フルテキストURL fulltext.pdf
著者 Sakata, Tatsuo| Watanabe, Akiharu| Takei, Nobuyuki| Obata, Takahiro| Hayashi, Shosaku| Shiota, Tetsuya| Nagashima, Hideo|
抄録

Hepatic ornithine decarboxylase and aniline hydroxylase activities increased as early as 6h after a single dosing of azathioprine tomale rats and reached maximum levels at the 12th h after the treatment. However, aniline hydroxylase activity dropped to levels mu lower than the cintrols 48 h following azathioprine treatment. The results are discussed with regard to the role of azqathioprine during the promotion of hepatocarcinogenesis.

キーワード azathioprine ornithine decarboxylase aniline hydroxylase hepatocarcinogesis
Amo Type Brief Note
出版物タイトル Acta Medica Okayama
発行日 1982-08
36巻
4号
出版者 Okayama University Medical School
開始ページ 317
終了ページ 320
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 7136860
Web of Science KeyUT A1982PE61100010
JaLCDOI 10.18926/AMO/30660
フルテキストURL fulltext.pdf
著者 Fujii, Masafumi| Namba, Tatsuji|
抄録

The activity and properties of cholinesterase (ChE) of the motor endplate and its fractions were studied in isolated human skeletal muscle. This preparation was used since the ChE activity of the membrane preparation was localized only in the motor endplate. The endplate ChE was stable in the isolated membrane for 4 weeks at 4 degrees C. The specific activity of the extracted ChE of human muscle membrane was 29.6% higher than that of the original membrane. Studies with specific substrates and ChE inhibitors indicated that most of the ChE of human muscle membrane and its fractions was acetylcholinesterase, and that the minor component was pseudocholinesterase. A Michaelis-Menten constant of 3.82 mM was estimated in the endplate ChE, and 0.88 mM in the extracted ChE of the endplate. The extracted human endplate ChE was separated into three fractions by Sephadex G-200 chromatography, and into two fractions by acrylamide gel electrophoresis.

キーワード acetycholinesterase cholinesterase moter endplates cholinesterase inhibitors isozymes
Amo Type Article
出版物タイトル Acta Medica Okayama
発行日 1982-08
36巻
4号
出版者 Okayama University Medical School
開始ページ 241
終了ページ 251
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 7136856
Web of Science KeyUT A1982PE61100001
JaLCDOI 10.18926/AMO/30659
フルテキストURL fulltext.pdf
著者 Watanabe, Akiharu| Obata, Takahiro| Nagashima, Hideo|
抄録

<p>plasma tyramine concentrations ewre abnormally high in cirrhotic patients with hepatic encephalopathy. Oral administration of berberine hydrochloride corrected hyperyraminemia in cirrhotics and also prevented the elevation of plasma tyramine levels following the oral tyrosine load, probably because of berberine-inhibition of bacterial tyrosine decarboxylase in the intestine. This is a new approach as Anti-amine therapy for cirrhotic patients</p>

キーワード tyramine berberine kiver cirrhosis tyrosine load
Amo Type Article
出版物タイトル Acta Medica Okayama
発行日 1982-08
36巻
4号
出版者 Okayama University Medical School
開始ページ 277
終了ページ 281
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 7136858
Web of Science KeyUT A1982PE61100004
JaLCDOI 10.18926/AMO/30658
フルテキストURL fulltext.pdf
著者 Hiramatsu, Yoshitake|
抄録

The microvasculature of peripheral nerves in dog and human samples was studied by an injection replica scanning electron microscope (SEM) method. The three-dimensional relationship of epineural and intraneural vessels was well demonstrated by this method. Epineural vessels were made of both longitudinal arterioles and venules and also a close meshwork of capillaries. In the intraneural microvasculature, longitudinal arterioles and venules made up the perineural vessels, and grouped capillaries corresponded to the fine vasculature of intrafascicular vessels. Between epineural and intraneural vessels, there was little anastomosis but nutrient arteries ran through the epineural vasculature into the intraneural vessels. There was little interconnection of vessels among the intraneural vasculature. It was postulated that the close meshwork of the epineural layer can resist pressure from outside the peripheral nerves but that longitudinal venules seemed to be affected by pressure and tension at the localized area.

キーワード microvasculature peripheral nerve injection replica SEM
Amo Type Article
出版物タイトル Acta Medica Okayama
発行日 1982-08
36巻
4号
出版者 Okayama University Medical School
開始ページ 263
終了ページ 275
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 7136857
Web of Science KeyUT A1982PE61100003
JaLCDOI 10.18926/AMO/30657
フルテキストURL fulltext.pdf
著者 Hattori, Yukio| Inaba, Kozo| Moriwaki, Akiyoshi| Hori, Yasuo|
抄録

A chronic epileptic focus not resulting in generalized convulsions was induced by a microinjection of FeCl3 solution into the left anterior cortex of rats. Cyclic AMP accumulation in response to adenosine was examined in incubated slices from the cerebral cortex of animals that showed bilateral spike and slow wave complex in an electrocorticogram (ECoG) 30 to 60 days after the microinjection. Cyclic AMP accumulation after incubation with adenosine was most marked in slices from the left anterior quadrant of the cortex including a FeCl3-injected site. A medium response was observed in both the left posterior and the right anterior quadrants, but little or no response in the right posterior quadrant of the cortex.

キーワード rat cerebral cortex iron-induced epileptic focus cyclic AMP adenosine
Amo Type Brief Note
出版物タイトル Acta Medica Okayama
発行日 1982-08
36巻
4号
出版者 Okayama University Medical School
開始ページ 313
終了ページ 316
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 6291336
Web of Science KeyUT A1982PE61100009
JaLCDOI 10.18926/AMO/30656
フルテキストURL fulltext.pdf
著者 Tagashira, Yoshiaki|
抄録

The local graft-versus-host (GvH) reaction in (C57BL/6 X BALB/c) F1 hybrid mice, assayed by popliteal lymph node enlargement, was specifically depressed by an injection of parental lymphocytes mixed with spleen cells from F1 mice pretreated with the same parental lymphocytes. Suppressor activity of CBF1 spleen cells was obtained 7 days after inoculation of parental lymphocytes, and peaked on day 10. The suppressive activity was induced by only spleen cells from CBF1 which was inoculated Balb/c lymphocytes, but not C57BL/6 lymphocytes. The lymphocyte subpopulation responsible for the suppressive activity was noticed in T cell population.

キーワード graft-vresus-host reaction suppressor cells CBF1 BALB/C
Amo Type Article
出版物タイトル Acta Medica Okayama
発行日 1982-08
36巻
4号
出版者 Okayama University Medical School
開始ページ 253
終了ページ 261
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 6215832
Web of Science KeyUT A1982PE61100002
JaLCDOI 10.18926/AMO/30655
フルテキストURL fulltext.pdf
著者 Nishioka, Keiko| Masuda, Yu| Nishizaki, Kazunori| Ogura, Yoshio|
抄録

Unilateral cystic inner ear anomalies were diagnosed in two siblings, a 9 year old boy and a 6 year old girl. X-ray examination of the temporal bone was performed, together with audiological examinations and vestibular function tests. The common tomographic X-ray findings consisted of an enlarged solitary sac type deformity of the vestibule with narrowing of the internal auditory canal, severe hypoplasia of the anterior semicircular canal and no visualized cochlea. Pure-tone audiometry revealed severe mixed type of hearing loss in the right ear in both children. The test for vestibular function showed no response to caloric testing.

キーワード inner ear anomaliy siblings
Amo Type Brief Note
出版物タイトル Acta Medica Okayama
発行日 1982-08
36巻
4号
出版者 Okayama University Medical School
開始ページ 321
終了ページ 324
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 7136861
Web of Science KeyUT A1982PE61100011
JaLCDOI 10.18926/AMO/30654
フルテキストURL fulltext.pdf
著者 Meguro, Tadamichi| Ogata, Masana|
抄録

Volume-time (V-T) and flow-volume (F-V) curves were measured in all the subjects of nonsmoking young males (mean value 26.3 yrs. of age), healthy and asthmatics. Eleven parameters of pulmonary function tests composed of two V-T, six F-V, and three mean time constant (MTC) parameters, were calculated from the curves. These parameters were used in the two analyses through the all possible selection procedure (APSP) discriminating between healthy adults and mild asthmatics and also between healthy and moderate. Flow rate at 75% of FVC (V75) proved to be the most useful parameter and V50 the next best in both analyses. The probability of misclassification using all eleven parameters was 19.64% in the analysis of healthy adults and mild asthmatics, and 4.29% in the analysis of healthy adults and moderate asthmatics. There was a little difference in the parameters selected at every step. The discriminant analysis proved that the flow-volume patterns were different according to the severity of bronchial asthma. Thus flow-volume recognition was considered to be important in analyzing the severity of bronchial asthma.

キーワード discriminant analysis the volume-time and flow-volume curve the all possible selection procedure (APSP) asthmatic severity and the probability of misclassification
Amo Type Article
出版物タイトル Acta Medica Okayama
発行日 1982-08
36巻
4号
出版者 Okayama University Medical School
開始ページ 299
終了ページ 306
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 7136859
Web of Science KeyUT A1982PE61100007
JaLCDOI 10.18926/AMO/30653
フルテキストURL fulltext.pdf
著者 Ito, Toshio| Itoshima, Tatsuya| Ukida, Minoru| Kiyotoshi, Shozo| Kawaguchi, Kenji| Ogawa, Hiromichi| Hattori, Shuzo| Kitadai, Masahiro|
抄録

The portal vein system was clearly visualized in superior mesenteric arterial portography using prostaglandin E1. Angiographic examination was performed in 68 patients with various liver diseases during the 2 year period from 1980 to 1981. Twenty microgram of prostaglandin E1 was injected into the superior mesenteric artery 30 seconds before injection of 60 ml of contrast medium. The main portal vein was visualized in all of 68 cases. A high rate of success for visualization of the intrahepatic portal vein system by prostaglandin E1 was achieved. The first branches of the intrahepatic portal vein were visualized in 100% of the cases, the second branches in 82%, the third branches in 44%, and the fourth branches in 4% in the right portal vein system. In the left portal vein system, the first branches were visualized in 87%, the second branches in 41%, and the third branches in 3% of the cases. The intrahepatic portal vein system was more clearly visualized in females than in males (P less than 0.05). This procedure is simple, safe and useful for clear visualization of the portal vein system.

キーワード superior mesenteric arteriography arterial portography portal vein system prostaglandin E1
Amo Type Article
出版物タイトル Acta Medica Okayama
発行日 1982-08
36巻
4号
出版者 Okayama University Medical School
開始ページ 291
終了ページ 297
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 6890302
Web of Science KeyUT A1982PE61100006
JaLCDOI 10.18926/AMO/30567
フルテキストURL fulltext.pdf
著者 Kimura, Ikuro| Yamana, Masatoshi| NNishishita, Akira| Sugiyama, Motoharu| Miyata, Akira|
抄録

A urinary iron excretion test was carried out in 22 patients with iron deficiency anemia. The iron excretion index was significantly higher in patients with intractable iron deficiency anemia compared with normal subjects and anemic patients who were responsive to iron therapy. The findings suggest that iron excretion may be a factor that modulates the response of patients to iron therapy.

キーワード iron excretion iron deficiency anemia saccharated iron oxide.
Amo Type Article
出版物タイトル Acta Medica Okayama
発行日 1980-02
34巻
1号
出版者 Okayama University Medical School
開始ページ 37
終了ページ 41
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 6446838
Web of Science KeyUT A1980JS13800005
JaLCDOI 10.18926/AMO/30566
フルテキストURL fulltext.pdf
著者 Miyamoto, Kanji| Miyano, Keiko| Miyoshi, Isao| Hamasaki, Kazuhide| Nishihara, Ryuji| Terao, Seiya| Kimura, Ikuro| Maeda, Kenichiro| Matsumura, Kazuyoshi| Mishijima, Katsumi| Tanaka, Toshio|
抄録

Cytogenetic studies were performed on a biopsy specimen of a jaw tumor and on a bone marrow aspirate from a Japanese patient with Epstein-Barr virus-negative Burkitt's lymphoma. A 14q + chromosome was found in cells from either source, although each contained a different clone. Other karyotypic abnormalities present in common included 2dir dup (1q) (q21 leads to q32), 3q+, 6p--, +12, +mar.

キーワード non-African Burkitt's lymphoma chromosome 14q+.
Amo Type Brief Note
出版物タイトル Acta Medica Okayama
発行日 1980-02
34巻
1号
出版者 Okayama University Medical School
開始ページ 61
終了ページ 65
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 6446841
Web of Science KeyUT A1980JS13800008
JaLCDOI 10.18926/AMO/30565
フルテキストURL fulltext.pdf
著者 Hidaka, Hideyuki| Omura, Sachiko| Oda, Takuzo|
抄録 Several DNA-histone complexes were reconstituted in the presence and absence of urea. The fiber size of DNA-histone H1 complex was about 20 A in width with knobs 100 to 250 A in diameter interspersed at an average interval of about 1,100 A. H1-was associated with DNA segments corresponding to a DNA size of fewer than 100 base pairs. DNA-histones H2A, H2B, H3 and H4 complex consisted of globular subunits 100 to 150 A in diameter alternating with thin strands, like beads on a string. DNA-whole histones complex was 200 to 250 A in width and had a condensed configuration. The nuclease digestion pattern of the complexes containing histones H2A, H2B, H3 and H4 was regular, similar to that of chromatin, and was disrupted by urea. The complex containing H1 was inactive for in vitro RNA synthesis by escherichia coli RNA polymerase, whereas the other complexes were active. The complexes reconstituted in the absence of urea had template activities slightly less than in the presence of urea.
キーワード finestructure template activity DNA-histone complex reconstitution reconstitution electron microscopy nuclease digestion.
Amo Type Article
出版物タイトル Acta Medica Okayama
発行日 1980-02
34巻
1号
出版者 Okayama University Medical School
開始ページ 1
終了ページ 10
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 6446834
Web of Science KeyUT A1980JS13800001
JaLCDOI 10.18926/AMO/30564
フルテキストURL fulltext.pdf
著者 Kimura, Ikuro| Sugiyama, Motoharu| Ishibashi, Ken| Miyata, Akira| Matsuzaka, Hiroto|
抄録 The effect of desferrioxamine on several hematological parameters was studied in growing rats. Animals given desferrioxamine intramuscularly (150 mg/kg daily) had increased urinary iron after one week, and decreased hemoglobin after 2 weeks. The difference in hemoglobin concentration between the treated and control groups was significant after 4 weeks of treatment with desferrioxamine, but the difference in the numbers of erythrocytes was not significant. The anemia was of hypochromic type. Desferrioxamine at this dosage did not retard growth. These findings support our previously reported concept of "iron-losing anemia".
キーワード desferrioxamine iron excretion iron deficiency anemia.
Amo Type Article
出版物タイトル Acta Medica Okayama
発行日 1980-02
34巻
1号
出版者 Okayama University Medical School
開始ページ 31
終了ページ 35
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 6446837
Web of Science KeyUT A1980JS13800004
JaLCDOI 10.18926/AMO/30563
フルテキストURL fulltext.pdf
著者 Yamada, Teruo| Talbot, Alan| Hirakawa, Misahisa| Kobayashi, Naohide| Shiwaku, Yoshitomo| Kosaka, Futami| Kimoto, Tetsuo|
抄録 Renal cortex was studied during experimentally induced ischemia. A transient increase in anerobic glycolysis occurred with concomitant swelling of both the Golgi apparatus and mitochondria. These intracytoplasmic organelles underwent marked changes in their intracellular positions. Infolding of cytoplasmic membrane at the basal side of proximal tubule cells increased in complexity and proceeded to enclose various intracytoplasmic microorganelles such as mitochondria and the Golgi apparatus. Piling up in layers was particularly marked around mitochondria. This piling up appeared as myelin-like structures on the free surface of, and within, proximal tubule cells, and followed disruption of the brush border at the free surface. Histological examination of thin sections showed that the fused portions of this brush border were actually brush border cytoplasmic membrane piled up in layers giving the appearance of myelin-like structures. After two hours of ischemia, parts of the membrane of these myelin-like structures were disrupted. Large vacuoles developed and these were thought to be related to the large vacuoles seen during cell degeneration.
キーワード Myelin-like structures ischemia kidney
Amo Type Article
出版物タイトル Acta Medica Okayama
発行日 1980-02
34巻
1号
出版者 Okayama University Medical School
開始ページ 19
終了ページ 30
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 6446836
Web of Science KeyUT A1980JS13800003
JaLCDOI 10.18926/AMO/30562
フルテキストURL fulltext.pdf
著者 Takaya, Yasumasa| Ota, Zensuke| Makino, Hirofumi| Kida, Keiko| Miyoshi, Akira| Hiramatsu, Makoto| Takahashi, Kayo| Ofuji, Tadashi|
抄録 Isolated and purified glomerular basement membranes (GBM) of normal and aminonucleoside (PAN) nephrosis rats were observed by electron microscopy after negative staining. Although GBM of normal rats appeared as a molecular sieve with uniform pores, GBM of nephrotic rats showed enlargement and elongation of the pores. For an average of fifty pores, the long dimension was 40.4+/-10.7 A and the short dimension 13.8+/-3.6 A in nephrosis whereas the long dimension was 12.3+/-2.5 A and the short dimension 8.4+/-1.0 A in normal rats. Changes in the pores in GBM were thought to result in increased permeability of serum protein and hence proteinuria.
キーワード gromerular basement membranc aminonucleoside nephrosis negative staining.
Amo Type Brief Note
出版物タイトル Acta Medica Okayama
発行日 1980-02
34巻
1号
出版者 Okayama University Medical School
開始ページ 67
終了ページ 70
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 6446842
Web of Science KeyUT A1980JS13800009
JaLCDOI 10.18926/AMO/30561
フルテキストURL fulltext.pdf
著者 Miyoshi, Masanori| Nakashima, Yusaku| Hirakawa, Shuzo| Mitsunaga, Mikio| Nakagawa, Osamu| Miura, Hiroshi| Suzuki, Shinya| Ofuji, Tadashi|
抄録

Function of pituitary-thyroid axis was studied in rats with experimentally induced thyroiditisWistar strain female rats were immunized with homologous thyroid extract in Freund's complete adjuvant and with simultaneous intradermal injection of pertussis vaccine concentrate. They received booster shots at the first and third week after the initial immunization. Serum thyroid hormones and TSH were measured just before, and at weekly intervals after, the initial immunization. Histological examination of the thyroid gland at the second week after immunization showed slight infiltration of macrophages in the thyroid follicles. From the third to the fourth week, massive lymphoid cell infiltration and destruction of follicular architecture developed in all immunized rats. Serum R3 levels slightly decreased during the second week, increased transiently during the third week, then decreased again thereafter. Serum T4 levels decreased slightly durinf the fourth week. Serum TSH levels were not elevated significantly during the third week, but the response to TRH was significantly increased at this time. Basal TSH levels were increased during the fourth week. The TRH test was a sensitive method capable of detecting minimal failure of thyroid function undetected by other routine measuremens of thyroid hormones and TSH.

キーワード thyroid function thyrotropin (TSH) experimental thyroiditis thyroid hormones.
Amo Type Article
出版物タイトル Acta Medica Okayama
発行日 1980-02
34巻
1号
出版者 Okayama University Medical School
開始ページ 11
終了ページ 17
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 6446835
Web of Science KeyUT A1980JS13800002
JaLCDOI 10.18926/AMO/30560
フルテキストURL fulltext.pdf
著者 Tsuji, Takao| Inoue, Junichi| Shinohara, Toru| Tsuchiya, Masao| Onoue, Kimiaki| Araki, Kiyonori| Nagashima, Hideo| Nakashima, Akira| Naito, Kunihiko|
抄録 Fragmented sheep red blood cell (SRBC) membrane was used for detection of T-cells in liver biopsy specimens from patients with chronic active hepatitis. SRBC was separated with Lymphoprep, sonicated, then filtered through a 3 mu Millipore-membrane as a fragmented SRBC reagent. Tissue T-cells were stained by an indirect immunofluorescent technique using SRBC reagent and fluorescein isothiocyanate (FITC)-labelled rabbit anti-SRBC. Positively staining lymphocytes were present in portal tracts and in areas of piecemeal necrosis. There also seemed to be a positive correlation between the number of positively staining lymphocytes and the activity of chronic hepatitis; numerous lymphocytes being stained in areas of severe piecemeal necrosis. Our findings suggest that the fragmented SRBC technique for detection of T-cells is reliable and reproducible, that it could be used as a clinical routine method, and that it is useful for further elucidating the nature of host immune reactions on tissue levels.<
キーワード T-lymphocyte T-cell lymphocyte receptor chronic active hepatitis immune response.
Amo Type Article
出版物タイトル Acta Medica Okayama
発行日 1980-02
34巻
1号
出版者 Okayama University Medical School
開始ページ 51
終了ページ 59
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 6446840
Web of Science KeyUT A1980JS13800007
JaLCDOI 10.18926/AMO/30559
フルテキストURL fulltext.pdf
著者 Asahi, Toshihiko| Matsumura, Yosuke| Tanahashi, Toyoko| Yoshimoto, Jun| Kaneshige, Tetsuzo| Fujita, Yukitoshi| Ohmori, Hiroyuki|
抄録 Seventy-eight patients were treated with intravesical instillation of Thio-Tepa in an attempt to prevent postoperative recurrences of bladder tumors. Fifty-six patients who were given no preventive treatment against recurrences were taken as the control group. The patients in this series presented at the Okayama University Hospital between 1961 and 1976 and only the first recurrence after the primary operation was taken into consideration. There was no significant difference in the recurrence rates of the control and instillation groups.
キーワード bladder tumor intravesical instillation thiotepa recurrence rate.
Amo Type Article
出版物タイトル Acta Medica Okayama
発行日 1980-02
34巻
1号
出版者 Okayama University Medical School
開始ページ 43
終了ページ 49
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 6446839
Web of Science KeyUT A1980JS13800006
JaLCDOI 10.18926/AMO/30558
フルテキストURL fulltext.pdf
著者 Yamada, Gotaro| Takahashi, Takashi| Mizuno, Motowo| Sakamoto, Yuji| Kaga, Kazumi| Kobayashi, Toshinari| Nagashima, Hideo|
抄録

A recently modified method using peroxidase labeled antibodies for light and electron microscopic demonstration of hepatitis B virus (HBV) was applied to the evaluation of hepatitis B surface antigen (HBsAg) on the surface of liver cells in biopsy specimens from 24HBsAg chronic carriers. Membranous distribution of HBsAg was demonstrated in diffuse or scattered hepatocytes in all 4 asymptomatic carriers and in 3 of the 20 patients with HBsAg-positive chronic active hepatitis or liver cirrhosis. In these patients with membranous expression of HBsAg, hepatitis B e antigen, Dane particles and DNA polymerase were often detected in sera, and large amounts of hepatitis B core antigen appeared in the liver. These results suggest that membrane-bound HBsAg may be expressed by the HBV genome. The ultrastructural study of liver cells showing membranous expression disclosed dense deposits of reaction product indicative of HBsAg on the cell membrane and/or on assembled particles within the extracellular space. In some hepatocytes showing both diffuse cytoplasmic and membranous expression of HBsAg, HBsAg-positive membrane of cisternae open to the intercellular space was connected with the liver cell membrane. These findings supported the conjecture that HBV associated antigens are integrated into the liver cell membrane.

キーワード type B hepatitis membrane-bound HBsAg immunoelectron microscopy peroxidase-labeled antibody method.
Amo Type Article
出版物タイトル Acta Medica Okayama
発行日 1980-06
34巻
3号
出版者 Okayama University Medical School
開始ページ 175
終了ページ 187
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 6447986
Web of Science KeyUT A1980KE59200005