JaLCDOI 10.18926/AMO/30493
フルテキストURL fulltext.pdf
著者 Teramoto, Norihito| Cao, liu| Kawasaki, Nobuhiro| Tonoyama, Yuji| Sarker, Ashit Baran| Yoshino, Tadashi| Takahashi, Kiyoshi| Akagi, Tadaatsu|
抄録 <p>&#60;P&#62;Reed-Sternberg cells (RS cells) of Hodgkin's disease (HD) are frequently infected with Epstein-Barr virus (EBV) and express EBV-encoded nonpolyadenylated RNA transcripts (EBER)-1. EBV latency has been classified into three distinct forms: Latency I, expressing only one of the latent proteins, EBV nuclear antigen (EBNA)-1, latency II, coexpressing EBNA-1 and LMPs, and latency III, expressing all latent viral proteins. RS cells express LMP-1 in addition to EBNA-1 and are considered to be EBV latency II frequently encountered in nasopharyngeal carcinoma. We examined 13 cases of EBV-infected HD by combined EBER-1 in situ hybridization and immunostaining for LMP-1. All of the RS cells expressed EBER-1, but a substantial number of EBER-1+ RS, cells were negative for LMP-1. The percentage of LMP-1+ RS cells out of EBER-1+ RS cells varied from 7% to 100% (average 69%). In this study, we showed that all EBV-infected RS cells were not restricted to latency II, and some belonged to latency I.&#60;/P&#62;</p>
キーワード in situ hybridization EBER-1 immunohistochemistry latecy
Amo Type Article
発行日 1996-10
出版物タイトル Acta Medica Okayama
50巻
5号
出版者 Okayama University Medical School
開始ページ 267
終了ページ 270
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 8914680
Web of Science KeyUT A1996VQ20600006
JaLCDOI 10.18926/AMO/30484
フルテキストURL fulltext.pdf
著者 Tonoyama, Yuji| Teramoto, Norihiro| Sarker, Ashit Baran| Yoshino, Tadashi| Hayashi, Kazuhiko| Takahashi, Kiyoshi| Akagi, Tadaatsu|
抄録 <p>To elucidate the latent state and reactivation of Epstein-Barr virus (EBV) in non-neoplastic lymphoid lesions, we investigated 144 non-neoplastic lymphoid lesions by in situ hybridization (ISH) to detect the expression of EBV-encoded small RNAs (EBER)-1 and BCRF-1 and by immunostaining for latent membrane protein (LMP)-1 and ZEBRA. ISH for EBER-1 detected EBER-1-positive cells (EPC) in 31 of the 144 examined lesions (22%). EPC were detected in 4 of 49 cases of nonspecific lymphoid hyperplasia, in 16 of 20 abscess-forming granulomatous lymphadenitis (AFGL), 5 of 25 Kikuchi's disease, and in 3 of 3 infectious mononucleosis. LMP-1 was expressed in 6 of 124 non-neoplastic lymphoid lesions (4.8%). LMP-1-positive cells were observed in 6 of the 31 EBER-1-positive cases (19%). EPC were detected significantly more frequently in LMP-1- and ZEBRA-positive specimens than in the LMP-1- and ZEBRA-negative specimens. BCRF-1 was expressed in 4 of 11 cases examined: 2 of 3 AFGL, 1 of 2 Kikuchi's disease, and in the 1 case of atypical lymphoid hyperplasia. This study suggests that Epstein-Barr virus is prevalent and can be reactivated in the lymph nodes effaced by destructive inflammation, such as AFGL. Such inflammation may provide a local milieu that is conducive for EBV to enter the lytic cycle.</p>
キーワード EBER-I BCRF-l LMP-l ZEBRA lymphoid lesion
Amo Type Article
発行日 1996-04
出版物タイトル Acta Medica Okayama
50巻
2号
出版者 Okayama University Medical School
開始ページ 89
終了ページ 96
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 8744934
Web of Science KeyUT A1996UJ08100005
関連URL http://ousar.lib.okayama-u.ac.jp/metadata/5331
JaLCDOI 10.18926/AMO/30452
フルテキストURL fulltext.pdf
著者 Moreira, Luis Fernando| Iwagaki, Hiromi| Watanabe, Kazuhiko| Yoshino, Tadashi| Fuchimoto, Sadanori| Orita, Kunzo|
抄録 <p>A rare gastrointestinal tract neoplasm, primary non-Hodgkin's B-cell lymphoma in a 39-year-old, asymptomatic woman is described. The tumor was originally localized in the rectum without evidence of any other lymphoma-involved organ and treated by curative surgical procedure associated with postoperative chemotherapy.</p>
キーワード primary lymphoma rectum surgical treatment
Amo Type Article
発行日 1990-10
出版物タイトル Acta Medica Okayama
44巻
5号
出版者 Okayama University Medical School
開始ページ 279
終了ページ 282
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 2260500
Web of Science KeyUT A1990EG00700008
JaLCDOI 10.18926/AMO/30447
フルテキストURL fulltext.pdf
著者 Yoshino, Tadashi| Hoshida, Yoshihiko| Murakami, Ichiro| Takahashi, Kiyoshi| Akagi, Tadaatsu|
抄録 <p>We have attempted to clarify the characteristics of monoclonal antibodies (MAbs) detecting lymphocyte subsets in fixed materials. We examined by means of flow cytometric technique influences of fixatives and reactivity with malignant lymphomas (MLs). Specific markers for T-cells were UCHL1 and OPD4, which reacted especially with helper/inducer T-cells. MT1 recognized almost all of T-cells from peripheral blood and tonsils, but reacted with a part of B-MLs. As for B-cell markers, L26 was the most reliable marker for B-MLs. L26 and MB1 antigens could not be detected on living cells flow cytometrically. LN1 reacted with a part of T-cells as well as B-cells, but fluorescent intensity of the former was apparently stronger than that of the latter. Although LN2 antigen was located mainly in the cytoplasm close to the nuclear membrane immunohistochemically, it could be detected on living cells flow cytometrically. LN2 positive cells belonged to B-cells in peripheral blood and tonsils. When fixed for relatively short time, B5 and buffered formalin were better for examining MAbs than non-buffered formalin and ethanol.</p>
キーワード monoclonal antibodies lymphocyte subset flow cytometry
Amo Type Article
発行日 1990-10
出版物タイトル Acta Medica Okayama
44巻
5号
出版者 Okayama University Medical School
開始ページ 243
終了ページ 250
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 1701954
Web of Science KeyUT A1990EG00700002
JaLCDOI 10.18926/AMO/30333
フルテキストURL fulltext.pdf
著者 Motoi, Makoto| Yoshino, Tadashi| Kawabata, Kenji| Ikehara, Ikuko| Ohsumi, Shozo| Ogawa, Katsuo|
抄録 <p>Using the peroxidase antiperoxidase (PAP) method, lysozyme (LZM) was shown to exist in normal, reactive and neoplastic cells belonging to the mononuclear phagocyte system (MPS), but was not detected in histiocytosis X cells. Immunostaining for cytoplasmic LZM by the PAP method is useful for identification of mononuclear phagocytes and for diagnosis of the diseases in which these cells participate.</p>
キーワード lysozyme PAP method mononuclear phagocyte system
Amo Type Article
発行日 1984-04
出版物タイトル Acta Medica Okayama
38巻
2号
出版者 Okayama University Medical School
開始ページ 125
終了ページ 133
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 6375269
Web of Science KeyUT A1984SN81800004
著者 佐藤 康晴| 吉野 正|
発行日 2010-04-01
出版物タイトル 岡山医学会雑誌
122巻
1号
資料タイプ 学術雑誌論文
著者 劉 克約| 森 秀治| 高橋 英夫| 友野 靖子| 和気 秀徳| 菅家 徹| 佐藤 康晴| 平賀 憲人| 足立 尚登| 吉野 正| 西堀 正洋|
発行日 2008-12-01
出版物タイトル 岡山医学会雑誌
120巻
3号
資料タイプ 学術雑誌論文
著者 吉野 正| 赤木 忠厚|
発行日 2001-04-28
出版物タイトル 岡山医学会雑誌
113巻
1号
資料タイプ 学術雑誌論文
著者 高田 尚良| 吉野 正|
発行日 2008-01-04
出版物タイトル 岡山医学会雑誌
119巻
3号
資料タイプ 学術雑誌論文
著者 中川 和彦| 野口 雄司| 奥村 英雄| 佐藤 修一郎| 田中 志幸| 下野 玄英| アリ・エルディブ アリ モハメド| 青江 基| 小野 俊朗| 上中 明子| 大原 信哉| 吉野 正| 山下 和城| 角田 司| 清水 信義| 中山 睿一|
発行日 2007-09-03
出版物タイトル 岡山医学会雑誌
119巻
2号
資料タイプ 学術雑誌論文