ID | 53419 |
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Miyaji, Takaaki
Advanced Science Research Center, Okayama University
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Kuromori, Takashi
Gene Discovery Research Group, RIKEN Center for Sustainable Resource Science
Takeuchi, Yu
Department of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Yamaji, Naoki
Institute of Plant Science and Resources, Okayama University
Yokosho, Kengo
Institute of Plant Science and Resources, Okayama University
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Shimazawa, Atsushi
Department of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Sugimoto, Eriko
Gene Discovery Research Group, RIKEN Center for Sustainable Resource Science
Omote, Hiroshi
Department of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
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Ma, Jian Feng
Institute of Plant Science and Resources, Okayama University
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Shinozaki, Kazuo
Gene Discovery Research Group, RIKEN Center for Sustainable Resource Science
Moriyama, Yoshinori
Advanced Science Research Center, Okayama University
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抄録 | Ascorbate is an antioxidant and coenzyme for various metabolic reactions in vivo. In plant chloroplasts, high ascorbate levels are required to overcome photoinhibition caused by strong light. However, ascorbate is synthesized in the mitochondria and the molecular mechanisms underlying ascorbate transport into chloroplasts are unknown. Here we show that AtPHT4;4, a member of the phosphate transporter 4 family of Arabidopsis thaliana, functions as an ascorbate transporter. In vitro analysis shows that proteoliposomes containing the purified AtPHT4;4 protein exhibit membrane potential- and Cl-dependent ascorbate uptake. The AtPHT4;4 protein is abundantly expressed in the chloroplast envelope membrane. Knockout of AtPHT4;4 results in decreased levels of the reduced form of ascorbate in the leaves and the heat dissipation process of excessive energy during photosynthesis is compromised. Taken together, these observations indicate that the AtPHT4;4 protein is an ascorbate transporter at the chloroplast envelope membrane, which may be required for tolerance to strong light stress.
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発行日 | 2015-01-05
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出版物タイトル |
Nature Communications
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巻 | 6巻
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出版者 | Nature Publishing Group
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ISSN | 2041-1723
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資料タイプ |
学術雑誌論文
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オフィシャル URL | http://dx.doi.org/10.1038/ncomms6928
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言語 |
英語
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著作権者 | © 2015 Macmillan Publishers Limited. All rights reserved. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
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論文のバージョン | publisher
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査読 |
有り
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