ID | 53419 |
FullText URL | |
Author |
Kuromori, Takashi
Takeuchi, Yu
Yamaji, Naoki
Shimazawa, Atsushi
Sugimoto, Eriko
Omote, Hiroshi
Kaken ID
researchmap
Shinozaki, Kazuo
|
Abstract | Ascorbate is an antioxidant and coenzyme for various metabolic reactions in vivo. In plant chloroplasts, high ascorbate levels are required to overcome photoinhibition caused by strong light. However, ascorbate is synthesized in the mitochondria and the molecular mechanisms underlying ascorbate transport into chloroplasts are unknown. Here we show that AtPHT4;4, a member of the phosphate transporter 4 family of Arabidopsis thaliana, functions as an ascorbate transporter. In vitro analysis shows that proteoliposomes containing the purified AtPHT4;4 protein exhibit membrane potential- and Cl-dependent ascorbate uptake. The AtPHT4;4 protein is abundantly expressed in the chloroplast envelope membrane. Knockout of AtPHT4;4 results in decreased levels of the reduced form of ascorbate in the leaves and the heat dissipation process of excessive energy during photosynthesis is compromised. Taken together, these observations indicate that the AtPHT4;4 protein is an ascorbate transporter at the chloroplast envelope membrane, which may be required for tolerance to strong light stress.
|
Published Date | 2015-01-05
|
Publication Title |
Nature Communications
|
Volume | volume6
|
Publisher | Nature Publishing Group
|
ISSN | 2041-1723
|
Content Type |
Journal Article
|
Official Url | http://dx.doi.org/10.1038/ncomms6928
|
language |
English
|
Copyright Holders | © 2015 Macmillan Publishers Limited. All rights reserved. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
|
File Version | publisher
|
Refereed |
True
|
DOI | |
PubMed ID | |
Web of Science KeyUT |