JaLCDOI 10.18926/AMO/30333
フルテキストURL fulltext.pdf
著者 Motoi, Makoto| Yoshino, Tadashi| Kawabata, Kenji| Ikehara, Ikuko| Ohsumi, Shozo| Ogawa, Katsuo|
抄録 <p>Using the peroxidase antiperoxidase (PAP) method, lysozyme (LZM) was shown to exist in normal, reactive and neoplastic cells belonging to the mononuclear phagocyte system (MPS), but was not detected in histiocytosis X cells. Immunostaining for cytoplasmic LZM by the PAP method is useful for identification of mononuclear phagocytes and for diagnosis of the diseases in which these cells participate.</p>
キーワード lysozyme PAP method mononuclear phagocyte system
Amo Type Article
発行日 1984-04
出版物タイトル Acta Medica Okayama
38巻
2号
出版者 Okayama University Medical School
開始ページ 125
終了ページ 133
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 6375269
Web of Sience KeyUT A1984SN81800004
JaLCDOI 10.18926/AMO/31765
フルテキストURL fulltext.pdf
著者 Ohtsuki, Yuji| Danbara, Yoshifumi| Takeda, Isao| Takahashi, Kiyoshi| Hayashi, Kazuhiko| Sonobe, Hiroshi| Yoshino, Tadashi| Akagi, Tadaatsu|
抄録 <p>Metaplastic bony tissue along with hyperplastic mucosal epithelium showing no atypism was detected in biopsy materials from a Yamada type I gastric polyp. The tissue was metaplastic woven bone associated with calcification. Histogenesis of the bone formation is as yet unknown. This is the first reported case of the presence of metaplastic bone accompanied by hyperplastic gastric mucosa so far.</p>
キーワード stomach hyperplastic polyp metaplastic bone histopathology
Amo Type Article
発行日 1987-02
出版物タイトル Acta Medica Okayama
41巻
1号
出版者 Okayama University Medical School
開始ページ 43
終了ページ 46
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 3105253
Web of Sience KeyUT A1987G146400007
JaLCDOI 10.18926/AMO/30886
フルテキストURL fulltext.pdf
著者 Akagi, Tadaatsu| Takata, Hiroshi| Yoshino, Tadashi| Teramoto, Norihiro| Yano, Shoki| Oka, Takashi|
抄録 <p>Co-cultivation of thymus and spleen cells of Fisher and Lewis rats with lethally irradiated MT-2 cells harboring human T-cell leukemia virus type I (HTLV-I) resulted in the establishment of lymphoid cell lines, FIRT-1, FIRS-1, LERT-1, and LERS-1, respectively. Cells of these cell lines had rat T-cell characters as demonstrated by the positive reaction to monoclonal antibodies (MAbs) to rat T cell antigens (Thy 1 and pan T). They lacked surface immunoglobulins and strongly expressed rat interleukin-2 receptor antigen (Tac) and Ia antigen. Karyotypic analysis revealed that they had the normal rat karyotype in early cultures, but showed marked aneuploidy after long cultivation. None of them expressed HTLV gag proteins (p19 and p24) or virus particles, but they contained HTLV-I proviral DNA monoclonally and weakly expressed pX gene products (p40x). They were not transplantable into syngeneic newborn rats.</p>
キーワード human T-cell leukemia virus rat T cell immortalization
Amo Type Article
発行日 1989-06
出版物タイトル Acta Medica Okayama
43巻
3号
出版者 Okayama University Medical School
開始ページ 143
終了ページ 151
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 2788356
Web of Sience KeyUT A1989AG01600002
JaLCDOI 10.18926/AMO/30854
フルテキストURL fulltext.pdf
著者 Akagi, Tadaatsu| Nose, Soichiro| Takahashi, Kiyoshi| Yoshino, Tadashi| Horie, Yasushi| Motoi, Makoto| Sonobe, Hiroshi| Enzan, Hideaki|
抄録 <p>In the human lymphoreticular system, the alpha and beta subunits of S-100 protein are found in ordinary monocyte-macrophages and non-phagocytic histiocytes such as Langerhans cells and interdigitating reticulum cells, respectively. The beta subunit is also present in some CD8+ T cells. In the present study, we investigated the ontogeny of these histiocytes and lymphocytes in humans. Yolk sacs and 4 to 21-week fetuses were examined immunohistochemically for the presence of S-100 protein subunits using antisera monospecific to each subunit. S-100 alpha + macrophages were present in the yolk sacs and the hepatic sinusoids of the 4th week embryos prior to bone marrow hematopoiesis. These macrophages later appeared in other lymphoid organs when anlagen of these organs were formed. No S-100 beta + cells were found in the yolk sacs. S-100 beta+ histiocytes were first detected in the hepatic sinusoids of the 5th week embryo, and after the 8th week of gestation, they were distributed in other lymphoid organs. S-100 beta+ lymphocytes were not found in the liver. They were first detected in the thymus at the 12th week of gestation, and were subsequently distributed in other lymphoid organs. These results suggest that S-100 beta+ lymphocytes and histiocytes may belong to different cell lineages, and the former may not be the precursor of the latter.</p>
キーワード S-100 protein ontogeny lymphocyte histiocyte
Amo Type Article
発行日 1989-08
出版物タイトル Acta Medica Okayama
43巻
4号
出版者 Okayama University Medical School
開始ページ 203
終了ページ 210
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 2678903
Web of Sience KeyUT A1989AP79100002
JaLCDOI 10.18926/AMO/30447
フルテキストURL fulltext.pdf
著者 Yoshino, Tadashi| Hoshida, Yoshihiko| Murakami, Ichiro| Takahashi, Kiyoshi| Akagi, Tadaatsu|
抄録 <p>We have attempted to clarify the characteristics of monoclonal antibodies (MAbs) detecting lymphocyte subsets in fixed materials. We examined by means of flow cytometric technique influences of fixatives and reactivity with malignant lymphomas (MLs). Specific markers for T-cells were UCHL1 and OPD4, which reacted especially with helper/inducer T-cells. MT1 recognized almost all of T-cells from peripheral blood and tonsils, but reacted with a part of B-MLs. As for B-cell markers, L26 was the most reliable marker for B-MLs. L26 and MB1 antigens could not be detected on living cells flow cytometrically. LN1 reacted with a part of T-cells as well as B-cells, but fluorescent intensity of the former was apparently stronger than that of the latter. Although LN2 antigen was located mainly in the cytoplasm close to the nuclear membrane immunohistochemically, it could be detected on living cells flow cytometrically. LN2 positive cells belonged to B-cells in peripheral blood and tonsils. When fixed for relatively short time, B5 and buffered formalin were better for examining MAbs than non-buffered formalin and ethanol.</p>
キーワード monoclonal antibodies lymphocyte subset flow cytometry
Amo Type Article
発行日 1990-10
出版物タイトル Acta Medica Okayama
44巻
5号
出版者 Okayama University Medical School
開始ページ 243
終了ページ 250
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 1701954
Web of Sience KeyUT A1990EG00700002
JaLCDOI 10.18926/AMO/30452
フルテキストURL fulltext.pdf
著者 Moreira, Luis Fernando| Iwagaki, Hiromi| Watanabe, Kazuhiko| Yoshino, Tadashi| Fuchimoto, Sadanori| Orita, Kunzo|
抄録 <p>A rare gastrointestinal tract neoplasm, primary non-Hodgkin's B-cell lymphoma in a 39-year-old, asymptomatic woman is described. The tumor was originally localized in the rectum without evidence of any other lymphoma-involved organ and treated by curative surgical procedure associated with postoperative chemotherapy.</p>
キーワード primary lymphoma rectum surgical treatment
Amo Type Article
発行日 1990-10
出版物タイトル Acta Medica Okayama
44巻
5号
出版者 Okayama University Medical School
開始ページ 279
終了ページ 282
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 2260500
Web of Sience KeyUT A1990EG00700008
JaLCDOI 10.18926/AMO/32184
フルテキストURL fulltext.pdf
著者 Hirasawa, Ryoto| Hashimoto, Hozo| Makino, Shinya| Suemaru, Shuso| Takao, Toshihiro| Ota, Zensuke| Hoshida, Yoshihiko| Yoshino, Tadashi| Akagi, Tadaatsu|
抄録 <p>A 46-year-old woman with acromegaly and hyperthyroidism due to a pituitary adenoma. She had high serum thyroid-stimulating hormone (TSH) levels and very high serum growth hormone (GH) levels. Transsphenoidal removal of the tumor, post-operative irradiation, frontal craniotomy for removal of residual tumor and large-dose bromocriptine therapy were carried out consecutively. After therapy, serum GH levels gradually decreased, but not to the normal range, and serum TSH levels remained at inappropriately normal levels. Using immunoperoxidase techniques, GH-, TSH- and follicle-stimulating hormone (FSH)-containing cells were demonstrated in the adenoma. A long-acting somatostatin analogue (SMS 201-995, 600 micrograms/day) suppressed the serum GH level to the normal range with a concomitant suppression of TSH. Furthermore, the paradoxical serum GH responses to TRH and LH-RH were slightly improved. No important subjective side-effects were noted. Therefore, SMS 201-995 appeared to be a very effective drug in this patient with a GH- and TSH-producing pituitary tumor.&#60;/P&#62;</p>
キーワード TSH- and GH - producing pituitary adenoma acromegaly heperthyroidism somatostatin analogue (SMS 201-995)
Amo Type Article
発行日 1991-04
出版物タイトル Acta Medica Okayama
45巻
2号
出版者 Okayama University Medical School
開始ページ 107
終了ページ 115
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 1867112
Web of Sience KeyUT A1991FL60800007
JaLCDOI 10.18926/AMO/32209
フルテキストURL fulltext.pdf
著者 Mukuzono, Hiroshi| Yoshino, Tadashi| Akagi, Tadaatsu|
抄録 <p>A monoclonal antibody (MAb), OPT1, reactive with T cells in formalin-fixed, paraffin-embedded tissue sections, has been identified through immunization with activated T cells from peripheral blood lymphocytes (PBL). The antibody is an IgG1 antibody as demonstrated by the Ouchterlony technique. By cytofluorometric analysis, almost all CD3+ lymphocytes and only a few CD20+ lymphocytes of peripheral blood expressed the OPT1 antigen. Nonhematolymphoid cell lines were negative for OPT1 by the immunoperoxidase staining using acetone-fixed cell lines. On the contrary, peripheral T cells, cells of two T cell lines out of four and a part of the cells of one B cell line out of two were positive for OPT1. The immunoperoxidase staining of paraffin-embedded tissue sections revealed that most of lymphocytes in T cell areas of lymph nodes expressed OPT1 antigen. Some lymphocytes in both cortex and medulla of the thymus and erythroid precursors of the bone marrow were OPT1+. In the malignant lymphoma series, approximately 90% of T cell lymphomas and 6% of B cell lymphomas reacted with OPT1. None of the Reed-Sternberg cells nor Hodgkin cells in Hodgkin's disease were positive. Consequently, OPT1 may be useful for the diagnosis and study of malignant lymphomas and other related lesions.&#60;/P&#62;</p>
キーワード monoclonal antibody OPTI T cells lymphocytes lymphoma
Amo Type Article
発行日 1991-06
出版物タイトル Acta Medica Okayama
45巻
3号
出版者 Okayama University Medical School
開始ページ 147
終了ページ 154
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 1891974
Web of Sience KeyUT A1991FV15000004
JaLCDOI 10.18926/AMO/32620
フルテキストURL fulltext.pdf
著者 Takata, Hiroshi| Yoshino, Tadashi| Hoshida, Yoshihiko| Takata, Ikuko| Akagi, Tadaatsu|
抄録 <p>A cell line of human lung large cell carcinoma (LCC) was established directly from the metastatic skin tumor tissue. The clinical course of the patient who carried this carcinoma was peculiar; generalized lymphadenopathy, histologically resembling Hodgkin's disease, was found as the first clinical symptom. The lung tumor was not discovered until the time of autopsy. This cell line (KaMi) grew adherent to culture vessels with the population doubling time of 20.6h, formed colonies in soft agars with efficiency of 22.6%, and formed tumors in athymic nude mice. The authenticity of KaMi was confirmed by chromosomal analysis and isoenzyme patterns. KaMi cells bore a strong resemblance to the original tumor cells which were composed of small spindle cells, large polygonal cells, and multinucleated giant cells. Immunohistochemically, KaMi cells showed a weak tendency to differentiate to squamous cells, and these immunohistochemical reactivities were almost compatible to those of the original tumor cells, but ultrastructurally, KaMi cells were more immature than the original ones. Treatment with several reagents could not augment a differentiation of KaMi cells. Cytokeratin profiles showed a tendency of squamous cell differentiation. KaMi cells may aid in elucidating the pathogenesis and biology of LCC and its relationship to other lung tumors. </p>
キーワード Large cell lung carcinoma cell line cytokeratin
Amo Type Article
発行日 1992-08
出版物タイトル Acta Medica Okayama
46巻
4号
出版者 Okayama University Medical School
開始ページ 257
終了ページ 264
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 1279943
Web of Sience KeyUT A1992JL44200005
JaLCDOI 10.18926/AMO/32636
フルテキストURL fulltext.pdf
著者 Kondo, Eisaku| Yoshino, Tadashi| Akagi, Tadaatsu| Hayashi, Kazuhiko| Takahashi, Kiyoshi|
抄録 <p>Southern blot hybridization was used to detect the rearrangement and amplification of five proto-oncogenes (bcl-2, bcl-1, c-myc, c-myb and c-Ha-ras) and one tumor suppressor gene (RB-1) in 55 Japanese patients with non-Hodgkin's lymphoma; 16 with T-cell lymphomas and 39 with B-cell lymphomas (7 follicular and 32 diffuse lymphomas). Genetic abnormalities of the proto-oncogenes were detected in 7 of the 55 (13%). Genetic abnormalities of bcl-2 plus other genes were detected in 5 of 7 cases of follicular lymphoma (71%), rearrangements of bcl-2 and c-myc, rearrangement of bcl-2 and amplification of c-myb. Genetic abnormalities were observed in only three cases of diffuse lymphoma. In each of 3 cases of B-cell lymphoma, one of the genes, blc-2 mbr, bcl-2 mcr and c-myc, was rearranged respectively. The incidence of genetic abnormalities in diffuse lymphomas (6.3%) was lower than that in follicular lymphomas. None of diffuse lymphomas had double oncogene abnormality. No abnormalities were found in RB-1, bcl-1, and Ha-ras. These findings suggest that follicular lymphomas are associated with some abnormalities of oncogenes not restricted to bcl-2 that facilitate growth which may be associated with their clinical features.</p>
キーワード malignant lymphoma cellular oncogenes
Amo Type Article
発行日 1992-12
出版物タイトル Acta Medica Okayama
46巻
6号
出版者 Okayama University Medical School
開始ページ 407
終了ページ 415
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 1485535
Web of Sience KeyUT A1992KE49600002
JaLCDOI 10.18926/AMO/31606
フルテキストURL fulltext.pdf
著者 Sarker, Ashit Baran| Akagi, Tadaatsu| Yoshino, Tadashi| Fujiwara, Kotaro| Nose, Soichiro|
抄録 <p>The distribution of lectin receptors in the human tonsil was studied using 16 biotinylated lectins. The avidin-biotin-peroxidase complex (ABC) method was used on frozen and paraffin-embedded tissue sections. Cell suspensions were also analysed by dual flow cytometry using respective fluorescein isothiocyanate-conjugated lectins and phycoerythrin-labeled anti-CD3 and anti-human immunoglobulin. Frozen sections fixed with acetone and paraffin-embedded materials fixed in three solutions were compared for lectin affinity; ethanol-fixed sections gave best results followed by frozen and buffered formalin-fixed ones, then nonbuffered formalin. Con-A, RCA-1, LcH, WGA, MPA, PHA, PSA, PNA, SJA and GSA-1 reacted with all tissue components of the tonsil in immunohistochemical studies, but binding intensity was fixative dependent. Binding of Lotus and BPA to lymphocytes was limited to germinal center lymphocytes. Other tissue components were also reactive but staining intensity was weaker in Lotus compared with BPA. SBA and DBA did not react with lymphocytes, but reacted with macrophages/histiocytes, vascular endothelia, and epithelial cells. LBA and LPA were constantly negative with all tissue components irrespective of fixatives. Flow cytometric analyses showed that all but three (DBA, LBA and LPA) partially or totally stained lymphocyte surfaces. Lotus receptors were expressed exclusively on B-lymphocytes.</p>
キーワード lectins ?histochemistry flow cytometry human tonsil
Amo Type Article
発行日 1993-02
出版物タイトル Acta Medica Okayama
47巻
1号
出版者 Okayama University Medical School
開始ページ 13
終了ページ 19
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 8460551
Web of Sience KeyUT A1993KP18500003
JaLCDOI 10.18926/AMO/31588
フルテキストURL fulltext.pdf
著者 Fujiwara, Kotaro| Yoshino, Tadashi| Miyake, Kenji| Ohara, Nobuya| Akagi, Tadaatsu|
抄録 <p>Lymphocyte adhesion molecules defined by anti-CD44 antibody (Hermes-3) may be involved in lymphocyte binding to high endothelial venules at sites where lymphocytes exist the blood. CD44 expression was immunohistochemically examined in 167 well characterized cases of malignant lymphomas (MLs). None of 12 nodal follicular lymphomas (FLs) were CD44+, whereas 3 of 4 extranodal ones showed distinct CD44 expression. In contrast to nodal FLs, 28 of the 38 (74%) nodal diffuse B-cell lymphomas were CD44+ (p < 0.0001). T-cell lymphomas showed a significantly higher expression of CD44 antigen than diffuse B-cell lymphomas in the nodal cases (p < 0.04), but not in the extranodal ones. In nodal diffuse lymphomas, 3 of 5 stage I lymphomas (60%) were CD44+ in contrast to 53 of 63 stage II-IV lymphomas (84%), but the difference was not statistically significant. Of 14 Hodgkin's diseases, 9 cases were CD44+ with no significant correlation with clinical stage. The data of flow cytometric analysis confirmed the results of immunohistochemical analysis. In conclusion, CD44 expression is relevant to primary sites of distinctive MLs originating in the mucosal regions (MALToma) and some histological subtypes, but the relation with clinical stage was not defined. Some other adhesion molecules or different mechanisms must also be taken into account concerning the genesis and the expansion of MLs.</p>
キーワード malignant lymphomas adhesion molecules CD44 clinical staging histological classification
Amo Type Article
発行日 1993-06
出版物タイトル Acta Medica Okayama
47巻
3号
出版者 Okayama University Medical School
開始ページ 215
終了ページ 222
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 8379348
Web of Sience KeyUT A1993LL12400011
JaLCDOI 10.18926/AMO/31108
フルテキストURL fulltext.pdf
著者 Miyatani, Katsuya| Takahashi, Kiyoshi| Yanai, Hiroyuki| Yoshino, Tadashi| Akagi, Tadaatsu|
抄録 <p>Previously, we reported that interleukin-2 (IL-2)-stimulated helper T cells produced an unknown soluble factor which induced dendritic cell-like differentiation in primary cultures of monocytic leukemia cells and we referred to this factor as dendritic cell differentiation factor (DCDF). In this study, we attempted to purify and characterize DCDF and investigated its biological effect on normal human monocytes. Gel filtration chromatography indicated that the molecular weight of DCDF is approximately 30-35 kDa. Chromatofocusing indicated that the isoelectric point of DCDF is approximately 5.0. DCDF, partially purified by subsequent gel filtration, chromatofocusing, and hydrophobic chromatography, significantly enhanced the HLA-DR expression of normal human monocytes and a human monocytic leukemia cell line, THP-1. This biological activity was not neutralized by any known antibodies to human cytokines. DCDF significantly amplified the T-cell stimulatory activity of monocytes in the allogeneic mixed leukocyte reaction (MLR). Moreover, DCDF significantly enhanced IL-1 beta and IL-6 production by monocytes in a dose-dependent manner. These results suggest that DCDF is a novel human cytokine which stimulates the accessory cell function of monocytes.</p>
キーワード dendritic cell differentiation protein purification cytokine
Amo Type Article
発行日 1994-04
出版物タイトル Acta Medica Okayama
48巻
2号
出版者 Okayama University Medical School
開始ページ 67
終了ページ 72
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 8042536
Web of Sience KeyUT A1994NJ77500001
JaLCDOI 10.18926/AMO/31124
フルテキストURL fulltext.pdf
著者 Ariki, Norifumi| Iwagaki, Hiromi| Yoshino, Tadashi| Nonaka, Yasuyuki| Fujiki, Shigeatsu| Perdomo, Jose Antonio| Hizuta, Akio| Tomoda, Jun| Tanaka, Noriaki| Tsuji, Takao| Orita, Kunzo|
抄録 <p>Endoscopical segmental piecemeal tumorectomy (ESPT) for nodular elevation of colorectal tumor is advantageous in terms of minimizing both surgical invasion and postoperative burden to the patients. Nodular elevation of colorectal tumors is said to occur when the body of the tumor is adenomatous and the surface of the focal cancer grows more horizontally into the lumen than vertically. We report here four cases of nodular elevation of colorectal tumors which were each treated by different surgical procedures.</p>
キーワード nodular elevation coloretal tumors endoscopical segmental piecemeal tumorectomy
Amo Type Article
発行日 1994-06
出版物タイトル Acta Medica Okayama
48巻
3号
出版者 Okayama University Medical School
開始ページ 169
終了ページ 171
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 7942075
Web of Sience KeyUT A1994NV04300009
JaLCDOI 10.18926/AMO/31091
フルテキストURL fulltext.pdf
著者 Teramoto, Norihiro| Tonoyama, Yuji| Akagi, Tadaatsu| Sarker, Ashit Baran| Yoshino, Tadashi| Yamadori, Ichiro| Takahashi, Kiyoshi|
抄録 <p>The sensitivity and specificity of single cell polymerase chain reaction (PCR) were studied. Its high sensitivity enabled detection of a single-copy gene, such as human T-lymphotropic virus type I genome in paraffin sections. The rate of obtaining positive signals with this method was affected by the number of copies of the gene in the target cell. Specificity was satisfactory if the procedure was properly and carefully followed. Since the single cell PCR is a time-consuming method which requires skill and experience to pick up the target cells accurately, the applicability of this method is limited. It works best when it is used to analyze a single or a few copy genes in histologically identified cells.</p>
キーワード polymerase chain reaction human T-lymphotropic virus type I paraffin section single cell single copy gene
Amo Type Article
発行日 1994-08
出版物タイトル Acta Medica Okayama
48巻
4号
出版者 Okayama University Medical School
開始ページ 189
終了ページ 193
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 7817773
Web of Sience KeyUT A1994PE51400003
JaLCDOI 10.18926/AMO/30484
フルテキストURL fulltext.pdf
著者 Tonoyama, Yuji| Teramoto, Norihiro| Sarker, Ashit Baran| Yoshino, Tadashi| Hayashi, Kazuhiko| Takahashi, Kiyoshi| Akagi, Tadaatsu|
抄録 <p>To elucidate the latent state and reactivation of Epstein-Barr virus (EBV) in non-neoplastic lymphoid lesions, we investigated 144 non-neoplastic lymphoid lesions by in situ hybridization (ISH) to detect the expression of EBV-encoded small RNAs (EBER)-1 and BCRF-1 and by immunostaining for latent membrane protein (LMP)-1 and ZEBRA. ISH for EBER-1 detected EBER-1-positive cells (EPC) in 31 of the 144 examined lesions (22%). EPC were detected in 4 of 49 cases of nonspecific lymphoid hyperplasia, in 16 of 20 abscess-forming granulomatous lymphadenitis (AFGL), 5 of 25 Kikuchi's disease, and in 3 of 3 infectious mononucleosis. LMP-1 was expressed in 6 of 124 non-neoplastic lymphoid lesions (4.8%). LMP-1-positive cells were observed in 6 of the 31 EBER-1-positive cases (19%). EPC were detected significantly more frequently in LMP-1- and ZEBRA-positive specimens than in the LMP-1- and ZEBRA-negative specimens. BCRF-1 was expressed in 4 of 11 cases examined: 2 of 3 AFGL, 1 of 2 Kikuchi's disease, and in the 1 case of atypical lymphoid hyperplasia. This study suggests that Epstein-Barr virus is prevalent and can be reactivated in the lymph nodes effaced by destructive inflammation, such as AFGL. Such inflammation may provide a local milieu that is conducive for EBV to enter the lytic cycle.</p>
キーワード EBER-I BCRF-l LMP-l ZEBRA lymphoid lesion
Amo Type Article
発行日 1996-04
出版物タイトル Acta Medica Okayama
50巻
2号
出版者 Okayama University Medical School
開始ページ 89
終了ページ 96
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 8744934
Web of Sience KeyUT A1996UJ08100005
関連URL http://ousar.lib.okayama-u.ac.jp/metadata/5331
JaLCDOI 10.18926/AMO/30509
フルテキストURL fulltext.pdf
著者 Yanai, Hiroyuki| Yoshino, Tadashi| Takahashi, Kiyoshi| Ninomiya, Yoshifumi| Akagi, Tadaatsu|
抄録 <p>Circulating hepatitis C virus (HCV) particles can be fractionated by means of differential flotation centrifugation. It is reported that in the bottom fraction HCV is in the form immune complexes, whereas in the top, it is free of antibodies. We evaluated the significance of circulating complex and free HCV in chronic hepatitis C, and assessed the relationship in terms of the response to interferon (IFN) therapy. We examined sera before, just after, and 1 year after administering IFN to 18 patients with chronic hepatitis C, 10 of whom responded (group CR), and 8 did not (group NR). The amounts of virus were similar between both groups before therapy. After differential flotation centrifugation with 1.063 g/ml of NaCl, the top and bottom fractions were assayed for HCV RNA. Before therapy, HCV RNA was detected in the top fraction in 1 of 10 in group CR, and in 6 of 8 in group NR (P &#60; 0.05, chi-square test). HCV RNA was positive in the bottom fraction of all samples. In a follow-up study of group NR, HCV RNA was detected in the top fraction in 3 of 8 just after IFN therapy, and in 7 of 8 after 1 year. This study suggests that the presence of HCV in the top fraction can predict a poor response to IFN therapy.</p>
キーワード IL-2R ??chain phorbol ester monocyte differentiation protein kinase
Amo Type Article
発行日 1996-06
出版物タイトル Acta Medica Okayama
50巻
3号
出版者 Okayama University Medical School
開始ページ 145
終了ページ 150
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 8805853
Web of Sience KeyUT A1996UU60400005
JaLCDOI 10.18926/AMO/30493
フルテキストURL fulltext.pdf
著者 Teramoto, Norihito| Cao, liu| Kawasaki, Nobuhiro| Tonoyama, Yuji| Sarker, Ashit Baran| Yoshino, Tadashi| Takahashi, Kiyoshi| Akagi, Tadaatsu|
抄録 <p>&#60;P&#62;Reed-Sternberg cells (RS cells) of Hodgkin's disease (HD) are frequently infected with Epstein-Barr virus (EBV) and express EBV-encoded nonpolyadenylated RNA transcripts (EBER)-1. EBV latency has been classified into three distinct forms: Latency I, expressing only one of the latent proteins, EBV nuclear antigen (EBNA)-1, latency II, coexpressing EBNA-1 and LMPs, and latency III, expressing all latent viral proteins. RS cells express LMP-1 in addition to EBNA-1 and are considered to be EBV latency II frequently encountered in nasopharyngeal carcinoma. We examined 13 cases of EBV-infected HD by combined EBER-1 in situ hybridization and immunostaining for LMP-1. All of the RS cells expressed EBER-1, but a substantial number of EBER-1+ RS, cells were negative for LMP-1. The percentage of LMP-1+ RS cells out of EBER-1+ RS cells varied from 7% to 100% (average 69%). In this study, we showed that all EBV-infected RS cells were not restricted to latency II, and some belonged to latency I.&#60;/P&#62;</p>
キーワード in situ hybridization EBER-1 immunohistochemistry latecy
Amo Type Article
発行日 1996-10
出版物タイトル Acta Medica Okayama
50巻
5号
出版者 Okayama University Medical School
開始ページ 267
終了ページ 270
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 8914680
Web of Sience KeyUT A1996VQ20600006
JaLCDOI 10.18926/AMO/30764
フルテキストURL fulltext.pdf
著者 Ino, Hideo| Hayashi, Kazuhiko| Yanai, Hiroyuki| Teramoto, Norihiro| Koirala, Tirtha Raj| Chen, Hong-Li| Oka, Takashi| Yoshino, Tadashi| Takahashi, Kiyoshi| Akagi, Tadaastu|
抄録 <p>A simian cell line, Si-IIA, harboring Epstein-Barr-virus (EBV) -related herpesvirus (Si-IIA-EBV), produces malignant lymphoma in rabbits when administered by intravenous inoculation. In this study, we analyzed the Si-IIA-EBV genome and compared it with human EBV and herpesvirus macaca fascicularis 1 (HVMF 1 ), which is associated with B-cell lymphoma developing in SIV-infected immunosuppressed monkeys. DNA from Si-IIA-EBV was amplified by the polymerase chain reaction using three different primer pairs complementary to human EBV (B95-8) DNA; two of the primer pairs covered part of the long internal repeat 1 region (IR 1) and the third covered part of the BRRF 1 region. Direct sequencing of the three PCR products revealed that Si-IIA-EBV DNA had about 82% nucleotide homology to the human EBV DNA in all three regions and 92.4% homology to HVMF1 in the IR1 region. The blotting pattern by Southern blot analysis was different between Si-IIA-EBV and human EBV.</p>
キーワード Epstein-Barr virus HVMF 1 lymphoma ?monkey cell line PCR
Amo Type Article
発行日 1997-08
出版物タイトル Acta Medica Okayama
51巻
4号
出版者 Okayama University Medical School
開始ページ 207
終了ページ 212
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 9284968
Web of Sience KeyUT A1997XU03200004