JaLCDOI | 10.18926/15311 |
---|---|
タイトル(別表記) | 抗原および Ca ionophore A23187 刺激時におけるアトピー型気管支喘息末梢血好塩基球の運動能 |
フルテキストURL | 064_043_051.pdf |
著者 | 谷崎 勝朗| 貴谷 光| 御船 尚志| 光延 文裕| 越智 浩二| 原田 英雄| 中川 二郎| 宗田 良| 高橋 清| 木村 郁郎| |
抄録 | We examined histamine release and morphological changes in basophilic granulocytes from atopic subjects, in response to stimulation with antigen and Ca ionophore A23187. 1. Histamine release and a reduction in the rumber of basophils were more rapid and greater in extent at an early stage of antigen stimulation compared with Ca ionophore A23187 stimulation. 2. Morphological changes in basophils, represented by increased motility, in terms of an increased ratio of short to long axis diameter (L/S ratio), as well as the increased frequency of basophils with localized granules and those with pseudopods, were more often observed antigen stimulation than with Ca ionophore A23187 stimulation. In contrast, morphogical changes in which basophils appeared swollen, showing an increased mean cell diameter and an increased frequency of cells with 5 vacuoles or more were more predominant with Ca ionophore A23187 than with antigen stimulation. The results obtained here show that bridging of IgE receptors is essential to activate basophils and induce increased motility in these cells. |
キーワード | histamine release (ヒスタミン遊離) increased basophil motility (好塩基球の運動能の亢進) bridging of IgE receptors (LgE 受容体) antigen (抗原) Ca ionophore A23187 |
出版物タイトル | 岡大三朝分院研究報告 |
発行日 | 1993-06 |
巻 | 64巻 |
開始ページ | 43 |
終了ページ | 51 |
ISSN | 0918-7839 |
言語 | 英語 |
論文のバージョン | publisher |
NAID | 120002307677 |
JaLCDOI | 10.18926/15341 |
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タイトル(別表記) | 抗原および抗ヒトIgEに対する好塩基球の反応性. 2.分泌機序における形態的変化 |
フルテキストURL | 063_128_133.pdf |
著者 | 谷崎 勝朗| 高橋 清| 木村 郁郎| |
抄録 | Morphological changes of blood basophils activated by antigen and anti-IgE were discussed in relation to release mechanisms of the cells. 1. Basophils activated by antigen and anti-IgE show two different types : A form (pear-shaped) and B from (swollen type), on smear preparartions. 2. Pear-shaped basophils(reactive basophils), which appear after stimulation by antigen and anti-IgE, can be observed by the staining solution for the direct count of basophils and eosinophils. 3. The incidence of reactive basophils increases after addition of antigen. Thus, observation of reactive basophils can be clinically applied for detecting allergen (DCRB ; direct connt of reactive basophils). 4. Observation of morphological basophil response by phase- contrast microscopic motion pictures demonstrates that basophils activated by antigen show two different reponses, increase in motility (oriented movement) and degranulation. |
キーワード | morphological changes of basophils (好塩基球の形態的変化) reactive basophils (反応好塩基球) degranulation (脱顆粒) histamine release (ヒスタミン遊離) |
出版物タイトル | 岡大三朝分院研究報告 |
発行日 | 1992-06 |
巻 | 63巻 |
開始ページ | 128 |
終了ページ | 133 |
ISSN | 0913-3771 |
言語 | 英語 |
論文のバージョン | publisher |
JaLCDOI | 10.18926/AMO/30359 |
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フルテキストURL | fulltext.pdf |
著者 | Tanizaki, Yoshiro| Komagoe, Haruki| Sudo, Michiyasu| Morinaga, Hiroshi| Kitani, Hikaru| Tada, Shinya| Takahashi, Kiyoshi| Kimura, Ikuro| |
抄録 | Anti-IgE-induced histamine release from basophils was examined in 46 asthmatic subjects using a whole blood method. Basophils from subjects less than 30 years old released more histamine than those from subjects aged 41 to 50. The age at onset of the disease also affected the reactivity of basophils to anti-IgE: basophils showed a high response in subjects whose age at onset was between 0 and 10 years, and low response in the subjects whose age at onset was between 41 and 50 years. There was a correlation between histamine release and serum IgE levels. However, individual dose-response curves of histamine release varied greatly in whom serum IgE levels were low. |
キーワード | histamine release whole blood patient age age a? onset serum IgE levels |
Amo Type | Article |
出版物タイトル | Acta Medica Okayama |
発行日 | 1984-06 |
巻 | 38巻 |
号 | 3号 |
出版者 | Okayama University Medical School |
開始ページ | 275 |
終了ページ | 280 |
ISSN | 0386-300X |
NCID | AA00508441 |
資料タイプ | 学術雑誌論文 |
言語 | 英語 |
論文のバージョン | publisher |
査読 | 有り |
PubMed ID | 6205544 |
Web of Science KeyUT | A1984SY25800008 |
著者 | 谷崎 勝朗| 駒越 春樹| 周藤 真康| 中郷 実雄| 森永 寛| 大谷 純| 多田 慎也| 高橋 清| 木村 郁郎| |
---|---|
発行日 | 1984-04-30 |
出版物タイトル | 岡山医学会雑誌 |
巻 | 96巻 |
号 | 3-4号 |
資料タイプ | 学術雑誌論文 |
著者 | 高橋 清| |
---|---|
発行日 | 1977-12-30 |
出版物タイトル | 岡山医学会雑誌 |
巻 | 89巻 |
号 | 11-12号 |
資料タイプ | 学術雑誌論文 |
著者 | 高橋 清| |
---|---|
発行日 | 1977-12-30 |
出版物タイトル | 岡山医学会雑誌 |
巻 | 89巻 |
号 | 11-12号 |
資料タイプ | 学術雑誌論文 |
著者 | 高橋 清| |
---|---|
発行日 | 1977-12-30 |
出版物タイトル | 岡山医学会雑誌 |
巻 | 89巻 |
号 | 11-12号 |
資料タイプ | 学術雑誌論文 |
著者 | 木村 郁郎| 谷崎 勝朗| 佐藤 周一| 斉藤 勝剛| 高橋 清| 上田 暢男| 小野 波津子| 守谷 欣明| |
---|---|
発行日 | 1975-02-28 |
出版物タイトル | 岡山医学会雑誌 |
巻 | 87巻 |
号 | 1-2号 |
資料タイプ | 学術雑誌論文 |
JaLCDOI | 10.18926/AMO/30325 |
---|---|
フルテキストURL | fulltext.pdf |
著者 | Tanizaki, Yoshiro| Komagoe, Haruki| Sudo, Michiyasu| Morinaga, Hiroshi| Kitani, Hikaru| Tada, Shinya| Takahashi, Kiyoshi| Kimura, Ikuro| |
抄録 | The correlation between blood eosinophilia and anti-IgE-mediated histamine release was investigated in 22 bronchial asthma patients with peripheral eosinophilia (over 8%). In the cases (Group A-1 and Group A-2) in which house dust was the specific antigen, significant histamine release from basophils was induced by anti-IgE and house dust. The result indicates a relationship between eosinophilia and the IgE-mediated mechanism of disease onset. In the cases (Group A-3) with RAST scores of 0+ and 1+ to house dust, the anti-IgE-induced histamine release varied from low to high percentages, and the participation of the IgE-mediated pathway was indicated in some cases. In the cases (Group B) with negative skin reactions, few patients had a family history of allergic disease. Their ages at onset were higher, and they demonstrated lower total IgE levels. These cases showed an extremely low percent of histamine release from basophils, which indicated the absence of a correlation between eosinophilia and IgE-mediated mechanisms. |
キーワード | blood eosinophilia histamine release IgE-mediated reaction asthma |
Amo Type | Article |
出版物タイトル | Acta Medica Okayama |
発行日 | 1984-12 |
巻 | 38巻 |
号 | 6号 |
出版者 | Okayama University Medical School |
開始ページ | 525 |
終了ページ | 531 |
ISSN | 0386-300X |
NCID | AA00508441 |
資料タイプ | 学術雑誌論文 |
言語 | 英語 |
論文のバージョン | publisher |
査読 | 有り |
PubMed ID | 6084401 |
Web of Science KeyUT | A1984TX98000005 |
JaLCDOI | 10.18926/AMO/30447 |
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フルテキストURL | fulltext.pdf |
著者 | Yoshino, Tadashi| Hoshida, Yoshihiko| Murakami, Ichiro| Takahashi, Kiyoshi| Akagi, Tadaatsu| |
抄録 | We have attempted to clarify the characteristics of monoclonal antibodies (MAbs) detecting lymphocyte subsets in fixed materials. We examined by means of flow cytometric technique influences of fixatives and reactivity with malignant lymphomas (MLs). Specific markers for T-cells were UCHL1 and OPD4, which reacted especially with helper/inducer T-cells. MT1 recognized almost all of T-cells from peripheral blood and tonsils, but reacted with a part of B-MLs. As for B-cell markers, L26 was the most reliable marker for B-MLs. L26 and MB1 antigens could not be detected on living cells flow cytometrically. LN1 reacted with a part of T-cells as well as B-cells, but fluorescent intensity of the former was apparently stronger than that of the latter. Although LN2 antigen was located mainly in the cytoplasm close to the nuclear membrane immunohistochemically, it could be detected on living cells flow cytometrically. LN2 positive cells belonged to B-cells in peripheral blood and tonsils. When fixed for relatively short time, B5 and buffered formalin were better for examining MAbs than non-buffered formalin and ethanol. |
キーワード | monoclonal antibodies lymphocyte subset flow cytometry |
Amo Type | Article |
出版物タイトル | Acta Medica Okayama |
発行日 | 1990-10 |
巻 | 44巻 |
号 | 5号 |
出版者 | Okayama University Medical School |
開始ページ | 243 |
終了ページ | 250 |
ISSN | 0386-300X |
NCID | AA00508441 |
資料タイプ | 学術雑誌論文 |
言語 | 英語 |
論文のバージョン | publisher |
査読 | 有り |
PubMed ID | 1701954 |
Web of Science KeyUT | A1990EG00700002 |
JaLCDOI | 10.18926/AMO/30493 |
---|---|
フルテキストURL | fulltext.pdf |
著者 | Teramoto, Norihito| Cao, liu| Kawasaki, Nobuhiro| Tonoyama, Yuji| Sarker, Ashit Baran| Yoshino, Tadashi| Takahashi, Kiyoshi| Akagi, Tadaatsu| |
抄録 | <P>Reed-Sternberg cells (RS cells) of Hodgkin's disease (HD) are frequently infected with Epstein-Barr virus (EBV) and express EBV-encoded nonpolyadenylated RNA transcripts (EBER)-1. EBV latency has been classified into three distinct forms: Latency I, expressing only one of the latent proteins, EBV nuclear antigen (EBNA)-1, latency II, coexpressing EBNA-1 and LMPs, and latency III, expressing all latent viral proteins. RS cells express LMP-1 in addition to EBNA-1 and are considered to be EBV latency II frequently encountered in nasopharyngeal carcinoma. We examined 13 cases of EBV-infected HD by combined EBER-1 in situ hybridization and immunostaining for LMP-1. All of the RS cells expressed EBER-1, but a substantial number of EBER-1+ RS, cells were negative for LMP-1. The percentage of LMP-1+ RS cells out of EBER-1+ RS cells varied from 7% to 100% (average 69%). In this study, we showed that all EBV-infected RS cells were not restricted to latency II, and some belonged to latency I.</P> |
キーワード | in situ hybridization EBER-1 immunohistochemistry latecy |
Amo Type | Article |
出版物タイトル | Acta Medica Okayama |
発行日 | 1996-10 |
巻 | 50巻 |
号 | 5号 |
出版者 | Okayama University Medical School |
開始ページ | 267 |
終了ページ | 270 |
ISSN | 0386-300X |
NCID | AA00508441 |
資料タイプ | 学術雑誌論文 |
言語 | 英語 |
論文のバージョン | publisher |
査読 | 有り |
PubMed ID | 8914680 |
Web of Science KeyUT | A1996VQ20600006 |
JaLCDOI | 10.18926/AMO/30509 |
---|---|
フルテキストURL | fulltext.pdf |
著者 | Yanai, Hiroyuki| Yoshino, Tadashi| Takahashi, Kiyoshi| Ninomiya, Yoshifumi| Akagi, Tadaatsu| |
抄録 | Circulating hepatitis C virus (HCV) particles can be fractionated by means of differential flotation centrifugation. It is reported that in the bottom fraction HCV is in the form immune complexes, whereas in the top, it is free of antibodies. We evaluated the significance of circulating complex and free HCV in chronic hepatitis C, and assessed the relationship in terms of the response to interferon (IFN) therapy. We examined sera before, just after, and 1 year after administering IFN to 18 patients with chronic hepatitis C, 10 of whom responded (group CR), and 8 did not (group NR). The amounts of virus were similar between both groups before therapy. After differential flotation centrifugation with 1.063 g/ml of NaCl, the top and bottom fractions were assayed for HCV RNA. Before therapy, HCV RNA was detected in the top fraction in 1 of 10 in group CR, and in 6 of 8 in group NR (P < 0.05, chi-square test). HCV RNA was positive in the bottom fraction of all samples. In a follow-up study of group NR, HCV RNA was detected in the top fraction in 3 of 8 just after IFN therapy, and in 7 of 8 after 1 year. This study suggests that the presence of HCV in the top fraction can predict a poor response to IFN therapy. |
キーワード | IL-2R ??chain phorbol ester monocyte differentiation protein kinase |
Amo Type | Article |
出版物タイトル | Acta Medica Okayama |
発行日 | 1996-06 |
巻 | 50巻 |
号 | 3号 |
出版者 | Okayama University Medical School |
開始ページ | 145 |
終了ページ | 150 |
ISSN | 0386-300X |
NCID | AA00508441 |
資料タイプ | 学術雑誌論文 |
言語 | 英語 |
論文のバージョン | publisher |
査読 | 有り |
PubMed ID | 8805853 |
Web of Science KeyUT | A1996UU60400005 |
JaLCDOI | 10.18926/AMO/30764 |
---|---|
フルテキストURL | fulltext.pdf |
著者 | Ino, Hideo| Hayashi, Kazuhiko| Yanai, Hiroyuki| Teramoto, Norihiro| Koirala, Tirtha Raj| Chen, Hong-Li| Oka, Takashi| Yoshino, Tadashi| Takahashi, Kiyoshi| Akagi, Tadaastu| |
抄録 | A simian cell line, Si-IIA, harboring Epstein-Barr-virus (EBV) -related herpesvirus (Si-IIA-EBV), produces malignant lymphoma in rabbits when administered by intravenous inoculation. In this study, we analyzed the Si-IIA-EBV genome and compared it with human EBV and herpesvirus macaca fascicularis 1 (HVMF 1 ), which is associated with B-cell lymphoma developing in SIV-infected immunosuppressed monkeys. DNA from Si-IIA-EBV was amplified by the polymerase chain reaction using three different primer pairs complementary to human EBV (B95-8) DNA; two of the primer pairs covered part of the long internal repeat 1 region (IR 1) and the third covered part of the BRRF 1 region. Direct sequencing of the three PCR products revealed that Si-IIA-EBV DNA had about 82% nucleotide homology to the human EBV DNA in all three regions and 92.4% homology to HVMF1 in the IR1 region. The blotting pattern by Southern blot analysis was different between Si-IIA-EBV and human EBV. |
キーワード | Epstein-Barr virus HVMF 1 lymphoma ?monkey cell line PCR |
Amo Type | Article |
出版物タイトル | Acta Medica Okayama |
発行日 | 1997-08 |
巻 | 51巻 |
号 | 4号 |
出版者 | Okayama University Medical School |
開始ページ | 207 |
終了ページ | 212 |
ISSN | 0386-300X |
NCID | AA00508441 |
資料タイプ | 学術雑誌論文 |
言語 | 英語 |
論文のバージョン | publisher |
査読 | 有り |
PubMed ID | 9284968 |
Web of Science KeyUT | A1997XU03200004 |
JaLCDOI | 10.18926/AMO/30854 |
---|---|
フルテキストURL | fulltext.pdf |
著者 | Akagi, Tadaatsu| Nose, Soichiro| Takahashi, Kiyoshi| Yoshino, Tadashi| Horie, Yasushi| Motoi, Makoto| Sonobe, Hiroshi| Enzan, Hideaki| |
抄録 | In the human lymphoreticular system, the alpha and beta subunits of S-100 protein are found in ordinary monocyte-macrophages and non-phagocytic histiocytes such as Langerhans cells and interdigitating reticulum cells, respectively. The beta subunit is also present in some CD8+ T cells. In the present study, we investigated the ontogeny of these histiocytes and lymphocytes in humans. Yolk sacs and 4 to 21-week fetuses were examined immunohistochemically for the presence of S-100 protein subunits using antisera monospecific to each subunit. S-100 alpha + macrophages were present in the yolk sacs and the hepatic sinusoids of the 4th week embryos prior to bone marrow hematopoiesis. These macrophages later appeared in other lymphoid organs when anlagen of these organs were formed. No S-100 beta + cells were found in the yolk sacs. S-100 beta+ histiocytes were first detected in the hepatic sinusoids of the 5th week embryo, and after the 8th week of gestation, they were distributed in other lymphoid organs. S-100 beta+ lymphocytes were not found in the liver. They were first detected in the thymus at the 12th week of gestation, and were subsequently distributed in other lymphoid organs. These results suggest that S-100 beta+ lymphocytes and histiocytes may belong to different cell lineages, and the former may not be the precursor of the latter. |
キーワード | S-100 protein ontogeny lymphocyte histiocyte |
Amo Type | Article |
出版物タイトル | Acta Medica Okayama |
発行日 | 1989-08 |
巻 | 43巻 |
号 | 4号 |
出版者 | Okayama University Medical School |
開始ページ | 203 |
終了ページ | 210 |
ISSN | 0386-300X |
NCID | AA00508441 |
資料タイプ | 学術雑誌論文 |
言語 | 英語 |
論文のバージョン | publisher |
査読 | 有り |
PubMed ID | 2678903 |
Web of Science KeyUT | A1989AP79100002 |
JaLCDOI | 10.18926/AMO/30874 |
---|---|
フルテキストURL | fulltext.pdf |
著者 | Sonobe, Hiroshi| Hayashi, Kazuhiko| Takahashi, Kiyoshi| |
抄録 | A case of ovarian leiomyoma is reported, together with histologic, immunohistologic and electron microscopic findings. A solid firm tumor, measuring 6.5 X 5 X 5 cm, was found in the right ovary of a 65-year-old woman. The tumor had an obvious whorled pattern on the cut-surface. Well-differentiated, long spindle-shaped neoplastic cells revealed positive immunoreactivity for anti-desmin. Ultrastructural observations included numerous microfilaments with dense patches in the cytoplasm, micropinocytotic vesicles beneath plasma membranes and continuous basal laminae around neoplastic cells. These findings were compatible with leiomyoma. The possible histogenesis of ovarian leiomyoma was discussed. |
キーワード | leiomyoma ovary immunohistochemistry ultrastructure |
Amo Type | Article |
出版物タイトル | Acta Medica Okayama |
発行日 | 1989-04 |
巻 | 43巻 |
号 | 2号 |
出版者 | Okayama University Medical School |
開始ページ | 131 |
終了ページ | 134 |
ISSN | 0386-300X |
NCID | AA00508441 |
資料タイプ | 学術雑誌論文 |
言語 | 英語 |
論文のバージョン | publisher |
査読 | 有り |
PubMed ID | 2728906 |
Web of Science KeyUT | A1989U578500008 |
JaLCDOI | 10.18926/AMO/30932 |
---|---|
フルテキストURL | fulltext.pdf |
著者 | Kimura, Ikuro| Tanizaki, Yoshiro| Sato, Shuichi| Saito, Katsuyoshi| Takahashi, Kiyoshi| Ueda, Nobuo| Ono, Hatsuko| Moritani, Yoshiaki| |
抄録 | The mechanism of action of the drug was investigated from various points of view. The findings may be summarized as follows: 1. In the experiments of the degranulation of mesenteric mast cells of rats, menaquinone proved to significantly inhibit the degranulation either in active or passive sensitization with the reagin-like antibody. 2. Menaquinone did not inhibit the formation of the reagin-like antibody. 3. In the experiements of the degranulation of basophilic granulocytes from patients of bronchial asthma, the rate of appearance of A form basophilic cells upon addition of the antihuman IgE goat serum was not markedly but significantly inhibited in the patients treated with menaquinone for long periods, as compared with that in the control, whereas the in vitro addition of menaquinone did not exert a significant inhibitory action. |
キーワード | vitamin K2 bronchial asthma |
Amo Type | Article |
出版物タイトル | Acta Medica Okayama |
発行日 | 1975-02 |
巻 | 29巻 |
号 | 1号 |
出版者 | Okayama University Medical School |
開始ページ | 73 |
終了ページ | 81 |
ISSN | 0386-300X |
NCID | AA00508441 |
資料タイプ | 学術雑誌論文 |
言語 | 英語 |
論文のバージョン | publisher |
査読 | 有り |
PubMed ID | 126001 |
NAID | 120002305330 |
JaLCDOI | 10.18926/AMO/30993 |
---|---|
フルテキストURL | fulltext.pdf |
著者 | Tanizaki, Yoshiro| Sudo, Michiyasu| Kitani, Hikaru| Araki, Hiroyuki| Oki, Kazuhiko| Tsuji, Mitsuaki| Takahashi, Kiyoshi| Kimura, Ikuro| |
抄録 | The arylsulfatase activity and histamine concentration of bronchoalveolar lavage fluid (BALF) were examined in patients with bronchial asthma in relation to the eosinophil count and asthma type (atopic and non-atopic). The BALF arylsulfatase activity and histamine concentration were significantly higher in atopic asthmatics than in non-atopic asthmatics. In atopic asthmatics, the activity of arylsulfatase was significantly increased in patients with a higher eosinophil count (10% or more). However, the BALF histamine concentration did not correlate with the eosinophil count. In non-atopic asthmatics, there was no significant correlation between arylsulfatase activity and the eosinophil count. The results show that arylsulfatase participates in IgE-mediated allergic reactions. |
キーワード | eosinophil count arylsulfatase histamine bronchoalveolar lavage fluid bronchial asthma |
Amo Type | Article |
出版物タイトル | Acta Medica Okayama |
発行日 | 1988-08 |
巻 | 42巻 |
号 | 4号 |
出版者 | Okayama University Medical School |
開始ページ | 227 |
終了ページ | 230 |
ISSN | 0386-300X |
NCID | AA00508441 |
資料タイプ | 学術雑誌論文 |
言語 | 英語 |
論文のバージョン | publisher |
査読 | 有り |
PubMed ID | 3177008 |
Web of Science KeyUT | A1988P884600006 |
JaLCDOI | 10.18926/AMO/31080 |
---|---|
フルテキストURL | fulltext.pdf |
著者 | Ozaki, Hiroshi| Mizutani, Machiko| Hayashi, Hiromitsu| Oka, Eiji| Ohtahara, Shunsuke| Kimoto, Hiroshi| Tanaka, Toshio| Hakozaki, Hand| Takahashi, Kiyoshi| Suzuki, Yoshiyuki| |
抄録 | We report the first case in Japan, i.e., the first case among oriental subject of Farber's disease. This is a rare disorder of lipid metabolism in infancy subsequent to a genetically-determined defect in ceramide degradation. Main features are characterized clinically by hoarseness, joint swelling, subcutaneous nodules and retarded psychomotor development. Lipid analysis and pathological investigation on the material obtained from a subcutaneous nodule confirmed clearly the presence of ceramide and intracytoplasmic inclusion bodies characteristic for Farber's disease. In this case, we experienced also corneal opacity and striking abnormalities in electroencephalogram, which have apparently not been noticed in the 17 cases hitherto reported. |
キーワード | Faeber's disease shpingolipid metablism infancy |
Amo Type | Article |
出版物タイトル | Acta Medica Okayama |
発行日 | 1978-04 |
巻 | 32巻 |
号 | 1号 |
出版者 | Okayama University Medical School |
開始ページ | 69 |
終了ページ | 79 |
ISSN | 0386-300X |
NCID | AA00508441 |
資料タイプ | 学術雑誌論文 |
言語 | 英語 |
論文のバージョン | publisher |
査読 | 有り |
PubMed ID | 148836 |
NAID | 120002313209 |
JaLCDOI | 10.18926/AMO/31091 |
---|---|
フルテキストURL | fulltext.pdf |
著者 | Teramoto, Norihiro| Tonoyama, Yuji| Akagi, Tadaatsu| Sarker, Ashit Baran| Yoshino, Tadashi| Yamadori, Ichiro| Takahashi, Kiyoshi| |
抄録 | The sensitivity and specificity of single cell polymerase chain reaction (PCR) were studied. Its high sensitivity enabled detection of a single-copy gene, such as human T-lymphotropic virus type I genome in paraffin sections. The rate of obtaining positive signals with this method was affected by the number of copies of the gene in the target cell. Specificity was satisfactory if the procedure was properly and carefully followed. Since the single cell PCR is a time-consuming method which requires skill and experience to pick up the target cells accurately, the applicability of this method is limited. It works best when it is used to analyze a single or a few copy genes in histologically identified cells. |
キーワード | polymerase chain reaction human T-lymphotropic virus type I paraffin section single cell single copy gene |
Amo Type | Article |
出版物タイトル | Acta Medica Okayama |
発行日 | 1994-08 |
巻 | 48巻 |
号 | 4号 |
出版者 | Okayama University Medical School |
開始ページ | 189 |
終了ページ | 193 |
ISSN | 0386-300X |
NCID | AA00508441 |
資料タイプ | 学術雑誌論文 |
言語 | 英語 |
論文のバージョン | publisher |
査読 | 有り |
PubMed ID | 7817773 |
Web of Science KeyUT | A1994PE51400003 |
JaLCDOI | 10.18926/AMO/31108 |
---|---|
フルテキストURL | fulltext.pdf |
著者 | Miyatani, Katsuya| Takahashi, Kiyoshi| Yanai, Hiroyuki| Yoshino, Tadashi| Akagi, Tadaatsu| |
抄録 | Previously, we reported that interleukin-2 (IL-2)-stimulated helper T cells produced an unknown soluble factor which induced dendritic cell-like differentiation in primary cultures of monocytic leukemia cells and we referred to this factor as dendritic cell differentiation factor (DCDF). In this study, we attempted to purify and characterize DCDF and investigated its biological effect on normal human monocytes. Gel filtration chromatography indicated that the molecular weight of DCDF is approximately 30-35 kDa. Chromatofocusing indicated that the isoelectric point of DCDF is approximately 5.0. DCDF, partially purified by subsequent gel filtration, chromatofocusing, and hydrophobic chromatography, significantly enhanced the HLA-DR expression of normal human monocytes and a human monocytic leukemia cell line, THP-1. This biological activity was not neutralized by any known antibodies to human cytokines. DCDF significantly amplified the T-cell stimulatory activity of monocytes in the allogeneic mixed leukocyte reaction (MLR). Moreover, DCDF significantly enhanced IL-1 beta and IL-6 production by monocytes in a dose-dependent manner. These results suggest that DCDF is a novel human cytokine which stimulates the accessory cell function of monocytes. |
キーワード | dendritic cell differentiation protein purification cytokine |
Amo Type | Article |
出版物タイトル | Acta Medica Okayama |
発行日 | 1994-04 |
巻 | 48巻 |
号 | 2号 |
出版者 | Okayama University Medical School |
開始ページ | 67 |
終了ページ | 72 |
ISSN | 0386-300X |
NCID | AA00508441 |
資料タイプ | 学術雑誌論文 |
言語 | 英語 |
論文のバージョン | publisher |
査読 | 有り |
PubMed ID | 8042536 |
Web of Science KeyUT | A1994NJ77500001 |