result 47501 件
| JaLCDOI | 10.18926/AMO/48076 |
|---|---|
| FullText URL | 66_1_7.pdf |
| Author | Kawauchi, Keiichiro| Watanabe, Masami| Kaku, Haruki| Huang, Peng| Sasaki, Kasumi| Sakaguchi, Masakiyo| Ochiai, Kazuhiko| Huh, Nam-ho| Nasu, Yasutomo| Kumon, Hiromi| |
| Abstract | The preclinical safety and therapeutic efficacy of adenoviral vectors that express the REIC/Dkk-3 tumor suppressor gene (Ad-REIC) was examined for use in prostate cancer gene therapy. The Ad-human (h) and mouse (m) REIC were previously demonstrated to induce strong anti-cancer effects in vitro and in vivo, and we herein report the results of two in vivo studies. First, intra-tumor Ad-hREIC administration was examined for toxicity and therapeutic effects in a subcutaneous tumor model using the PC3 prostate cancer cell line. Second, intra-prostatic Ad-mREIC administration was tested for toxicity in normal mice. The whole-body and spleen weights, hematological and serum chemistry parameters, and histological evaluation of tissues from throughout the body were analyzed. Both experiments indicated that there was no significant difference in the examined parameters between the Ad-REIC-treated group and the control (PBS- or Ad-LacZ-treated) group. In the in vitro analysis using PC3 cells, a significant apoptotic effect was observed after Ad-hREIC treatment. Confirming this observation, the robust anti-tumor efficacy of Ad-hREIC was demonstrated in the in vivo subcutaneous prostate cancer model. Based on the results of these preclinical experiments, we consider the adenovirus-mediated REIC/Dkk-3 in situ gene therapy to be safe and useful for the clinical treatment of prostate cancer. |
| Keywords | REIC Dickkopf-3 gene therapy prostate cancer preclinical study |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2012-02 |
| Volume | volume66 |
| Issue | issue1 |
| Publisher | Okayama University Medical School |
| Start Page | 7 |
| End Page | 16 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | CopyrightⒸ 2012 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 22358134 |
| Web of Science KeyUT | 000300800700002 |
| JaLCDOI | 10.18926/AMO/48075 |
|---|---|
| FullText URL | 66_1_1.pdf |
| Author | Udono, Heiichiro| |
| Abstract | Dendritic cells (DCs) take up soluble- or cell-associated antigens and digest them, delivering fragments to the MHC class I pathway to display antigenic peptides to CD8＋ T cells, a process known as cross-presentation. The pathway requires that, in order to be degraded by proteosomes, the extracellular antigens must have access to the cytosol across the endosomal membranes. Although the cross-presentation phenomena was first identified in the 1970s, the molecular mechanism responsible for the translocation is still not fully understood. In this context, we have recently found that cytosolic heat shock protein (HSP)90 translocates internalized antigen to the cytosol in DCs. Our results revealed the important role that cytosolic HSP90 plays in cross-presentation by pulling out endosomal antigen to the cytosol. |
| Keywords | heat shock protein 90 dendritic cells cross-presentation proteasome cytotoxic T cell immunity |
| Amo Type | Review |
| Publication Title | Acta Medica Okayama |
| Published Date | 2012-02 |
| Volume | volume66 |
| Issue | issue1 |
| Publisher | Okayama University Medical School |
| Start Page | 1 |
| End Page | 6 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | CopyrightⒸ 2012 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 22358133 |
| Web of Science KeyUT | 000300800700001 |
| Author | 岡山医学会| |
|---|---|
| Published Date | 1918-10-31 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume30 |
| Issue | issue345 |
| Content Type | Others |
| Author | 岡山医学会| |
|---|---|
| Published Date | 1918-10-31 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume30 |
| Issue | issue345 |
| Content Type | Others |
| Author | 横川 定| 末盛 進| |
|---|---|
| Published Date | 1918-10-31 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume30 |
| Issue | issue345 |
| Content Type | Journal Article |
| Author | 吉田 千束| |
|---|---|
| Published Date | 1918-10-31 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume30 |
| Issue | issue345 |
| Content Type | Journal Article |
| Author | 林 昇| 富岡 敏哉| |
|---|---|
| Published Date | 1918-10-31 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume30 |
| Issue | issue345 |
| Content Type | Journal Article |
| Author | 渡邊 善朝| |
|---|---|
| Published Date | 1918-10-31 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume30 |
| Issue | issue345 |
| Content Type | Journal Article |
| Author | 上坂 熊勝| 關 正次| |
|---|---|
| Published Date | 1918-10-31 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume30 |
| Issue | issue345 |
| Content Type | Journal Article |
| Author | 岡山医学会| |
|---|---|
| Published Date | 1918-09-30 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume30 |
| Issue | issue344 |
| Content Type | Others |
| Author | 岡山医学会| |
|---|---|
| Published Date | 1918-09-30 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume30 |
| Issue | issue344 |
| Content Type | Others |
| Author | 田邊 郁郎| |
|---|---|
| Published Date | 1918-09-30 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume30 |
| Issue | issue344 |
| Content Type | Journal Article |
| Author | 横川 定| 末盛 進| |
|---|---|
| Published Date | 1918-09-30 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume30 |
| Issue | issue344 |
| Content Type | Journal Article |
| Author | 天川 保| |
|---|---|
| Published Date | 1918-09-30 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume30 |
| Issue | issue344 |
| Content Type | Journal Article |
| Author | 野上 尚志| |
|---|---|
| Published Date | 1918-09-30 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume30 |
| Issue | issue344 |
| Content Type | Journal Article |
| Author | 岡山医学会| |
|---|---|
| Published Date | 1918-08-31 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume30 |
| Issue | issue343 |
| Content Type | Others |
| Author | 高橋 金一郎| |
|---|---|
| Published Date | 1918-08-31 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume30 |
| Issue | issue343 |
| Content Type | Article |
| Author | 横川 定| |
|---|---|
| Published Date | 1918-08-31 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume30 |
| Issue | issue343 |
| Content Type | Journal Article |
| Author | 中田 理吉| |
|---|---|
| Published Date | 1918-08-31 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume30 |
| Issue | issue343 |
| Content Type | Journal Article |
| Author | 吉田 千束| |
|---|---|
| Published Date | 1918-08-31 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume30 |
| Issue | issue343 |
| Content Type | Journal Article |
