result 5666 件
| Author | Zhao, Ying| |
|---|---|
| Published Date | 2007-03-23 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| Author | Uchida, Akiko| |
|---|---|
| Published Date | 2007-03-23 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| JaLCDOI | 10.18926/11763 |
|---|---|
| Title Alternative | Immunological Abnormalities of Alveolar Macrophages in Patients with Sarcoidosis |
| FullText URL | 001_039_050.pdf |
| Author | Nakata, Yasunari| |
| Abstract | Bronchoalveolar lavage is an invaluable means of accurately evaluating the inflammatory and immune processes in the human lung. Sarcoidosis is a multisystem disorder characterized by heightened immune processes at sites of disease activity. The lung is most commonly involved. Although granulomas are charactaristic pathologic features of this disease, a number of studies suggest that the initial lesion in the lung is a T-cell alveolitis (an accumulation of T-cellls in the lung). There are a lot of findings that show abnormal functions of alveolar macrophages obtained by bronchoalveolar lavage in the release of various monokines and arachidonic acid metabolites and metabolize oxygen. In this review, the abnormalities of alveolar macrophages implicated in pulmonary T-cell alveolitis and fibrosis are reviewed and their potential roles in the lungs are discussed. |
| Keywords | sarcoidosis alveolar macrophage monokine metabolize oxygen arachidonic acid metabolite |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1991-03-25 |
| Volume | volume1 |
| Start Page | 39 |
| End Page | 50 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313772 |
| Author | Harada, Daisuke| |
|---|---|
| Published Date | 2007-03-23 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| JaLCDOI | 10.18926/11759 |
|---|---|
| Title Alternative | 長期ホルマリン固定により失活したProliferating Cell Nuclear Antigen (PCNA) の免疫反応性回復条件の基礎的検討 ―マイクロウェーブ、オートクレーブの影響について― |
| FullText URL | 007_1_009_015.pdf |
| Author | Sakiyama, Junko| Ichimura, Mitsuko| Tohge, Hiroko| Endo, Hiroshi| Kawakami, Kaori| Kawatani, Yuki| |
| Abstract | Using paraffin-embedded tissue sections of liver cancer obtained from autopsy which had been preserved in 10% buffered formalin solution for 6 months while PCNA immunoreactivity was lost, we examined the effects of heat processing by either microwave(MW) and autoclave(AC) in the presence of various processing solution. It appeared that AC processing took shorter time period than MW irradiation to restore equal immunoreactivity. With regard to immunoreactivity retrieval by MW irradiation,however, variation of the degree of retrieval depending on processing time was smaller than in AC, and so the stable consequences were obtained. Although AC processed tissues tended to be stained deep, prolonged processing time presented strong background staining and blurred nuclear margins which made it difficult to estimate the positive cell count. As for the effects of processing solution, there was little difference in retrieval of PCNA among 0.01 M citrate buffer (pH 6.0), saturated solution of lead thiocyanate and distilled water, but the least background staining was observed with distilled water. These observations suggest that MW irradiation of which effect of retrieval is less dependent of processing time and with the least background stainability, is superior to AC processing for PCNA immunoreactivity retrieval on formalin-fixed tissues. |
| Keywords | PCNA microwave (マイクロウェーブ) autoclave (オートクレーブ) immunohistological staining (免疫組織染色) formalin fixation (ホルマリン固定) |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1996-09-30 |
| Volume | volume7 |
| Issue | issue1 |
| Start Page | 9 |
| End Page | 15 |
| ISSN | 0917-4494 |
| language | English |
| File Version | publisher |
| NAID | 120002313519 |
| JaLCDOI | 10.18926/11757 |
|---|---|
| Title Alternative | Lipid Peroxides (TBA reactive substances)and Fatty Acid Compositions in Mouse Serum with Whole-body Irradiation and in Tumor-bearing Mouse Serum with Local Irradiation |
| FullText URL | 001_029_037.pdf |
| Author | Yamamoto, Goki| |
| Abstract | Effects of irradiation on lipid peroxides of mouse serum, Ehrlich solid tumor-bearing mouse serum, which tomor cells were transplanted to the leg, and its tumor tissue were studied by a thiobarbituric acid (TBA) color reaction in the acetic acid condition with (Fe-TBA value) or without (TBA value) ferrous ammonium sulfate. TBA reactive substances were caluculated into the amount of malondialdehyde. Besides, fatty acid and lipid compositions were analyzed as a substrates. Irradiated samples were isolated at 3 days after irradiations. The TBA value of normal mouse serum was expressed 17nmoles/ml of serum, and Fe-TBA value gave 2.4-fold of the TBA value. Although an increase TBA value was not observed by whole-body irradiation, a significant increase of Fe-TBA value was measured, indicating 2.5-fold at 10Gy exposure compared with the normal Fe-TBA value, and 5.5-fold with the TBA value. The TBA value of tumor-bearing mouse serum was 14nmoles/ml as a low rate to that of normal serum, but the Fe-TBA value gave same magnification of that of normal one. The TBA value of tumor-bearing mouse serum was not changed by the local irradiation to the tumor region of leg, but the Fe-TBA value was increased 2.8 and 4.4 times at 10Gy and 20Gy exposures, respectively, comparing with the non-irradiated one, and 7.7 and 10.5 times with the TBA value, respectively. The TBA value of solid tumor homogenate exhibited 1.16nmoles/mg of protein, and the Fe-TBA value, pointed out 5 times as much as the TBA value. Both values slightly increased by the irradiation. These facts suggest that a shift of TBA value of serum hard to get, but Fe-TBA value of serum distinctly increases by the irradiation. The fatty acid composition of mouse serum lipids showed an increment of relative percentages of linoleic and arachidonic acids by the whole-body irradiation. The relative percentage of fatty acid compositon of lipids from solid tumor-bearing mouse serum was similar tendency to that of normal one, and the local exposure to the tumor part was not affected the each percentage. The percentage of high unsaturated fatty acid of tumor lipids similar to that of serum lipids, and a decrease of the percentage of arachidonic acid was accounted by the irradiation. In relative percentages of lipid compositions, the percentage of choleste rolester of serum lipids increased by the whole-body irradiation, and that of phospholipid fraction was not changed. In the case of tumor-bearing mouse serum lipids, an increment of the percentage of cholesterolester was obtained as compared with that of normal serum lipids, and this percentage decreased by the local irradiation to tumor part with an increment of the percentage of phospholipid. The percentage of phospholipid in tumor lipids had decreased by the irradiation. The facts suggest that a cause of the increased Fe-TBA value of tumor-bearing mouse serum by the irradiation to tumor is due to the release of peroxidizable phospholipid into serum from the damaged menbranes of tumor cells at large doses to exposure. |
| Keywords | Lipid peroxides Irradiation Mouse serum Tumor-bearing mouse serum Fatty acid composition |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1991-03-25 |
| Volume | volume1 |
| Start Page | 29 |
| End Page | 37 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313903 |
| JaLCDOI | 10.18926/11753 |
|---|---|
| Title Alternative | The effects of various fixating buffer soution in the electron microscopic observations |
| FullText URL | 006_055_061.pdf |
| Author | Akatsuka, Kazuya| |
| Abstract | 生物学的あるいは生化学的な緩衝液を使用して作製された電子顕微鏡試料と従来の電子顕微鏡用として使用されている緩衝液で作成した試料とで電子顕微鏡像から比較検討した。HEPES緩衝液で作製した試料の電子顕微鏡像は、従来の電子顕微鏡に用いられている緩衝液で作製した試料から得られた電子顕微鏡像と非常によく似た像を示し、このHEPES緩衝液は電子顕微鏡の固定用緩衝液として使用できることが認められたが、その他（PIPES、MOPS緩衝液）の緩衝液ではあまりにも細胞内の可溶性物質が溶出しすぎて使用に耐えなかった。電子顕微鏡学研究において使用されうる固定用緩衝液は細胞内のオルガネラの保持が余りにも良い場合には、その微細構造の解析が困難になり、程々に細胞内の可溶性物質が溶出した方が解析がし易くなると思われ、この様な観点から、今回使用したHEPES緩衝液は生物学的あるいは生化学的な研究から引き続き電子顕微鏡用の試料を作製する際、同じ緩衝液が使用できることが認められた。 |
| Keywords | 電子顕微鏡 (electron microscopy) 微細構造 (ultrastructure) 肝細胞 (liver cell) カコジル酸緩衝液 (cacodylate buffer) HEPES緩衝液 (HEPES buffer) |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1996-02-29 |
| Volume | volume6 |
| Start Page | 55 |
| End Page | 61 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313640 |
| JaLCDOI | 10.18926/11750 |
|---|---|
| Title Alternative | Total Skin Electron Beam Therapy |
| FullText URL | 001_001_006.pdf |
| Author | Nakagiri, Yoshitada| Inamura, Keiji| Miyake, Hideaki| Tahara, Seiji| Mimura, Seiichi| Egusa, Tomomi| Mikami, Yasutaka| Yamada, Toshiharu| Sugita, Katsuhiko| Hiraki, Yoshio| |
| Abstract | The peripherally T-cell lymphoma; Mycosis fungoides etc, has the good radiation sensitivity, and has been adapted for total skin electron beam therapy. In this study the pendular irradiation method was used for the purpose of total skin electron beam therapy in Mycosis fungoides, and physical data on the radiation field and the electron beam energy were useful clinically. |
| Keywords | 電子線全身照射法 菌状息肉症 全身性皮膚疾患 |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1991-03-25 |
| Volume | volume1 |
| Start Page | 1 |
| End Page | 6 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313887 |
| JaLCDOI | 10.18926/11749 |
|---|---|
| Title Alternative | メキシコ肺吸虫のセルカリアはどれか？ |
| FullText URL | 010_2_063_067.pdf |
| Author | Tongu, Yasumasa| |
| Abstract | The true cercaria of Paragonimus mexicanus is the cercaria reported by Malek et al. in 1985 as a cercaria of P. peruvianus. However, there is a question whether the flame cell formula of 2〔(3+3+3+3+3)+(3+3+3+3+3)〕=60 is justified. Therefore, I question the possibility of the cercaria of P. mexicanus reported by Ito et al. in 1985. If it has a pseudo-sucker, it belongs to another species of Paragonimus cercaria. The cercaria reported by Tongu et al. in 1990 from Venezuela was eliminated the possibility of a new species. It is probable that this cercaria belongs to P. mexicanus. Ibanez revived the name of P. peruvianus in 1990 by the differences in the cercaria and adults. However, I disagree with his opinion. |
| Keywords | Paragonimus mexicanus (メキシコ肺吸虫) Paragonimus peruvianus (ペルー肺吸虫) cercaria (セルカリア) taxonomy (分類) Latin America (中南米) |
| Publication Title | 岡山大学医学部保健学科紀要 |
| Published Date | 2000-03-24 |
| Volume | volume10 |
| Issue | issue2 |
| Start Page | 63 |
| End Page | 67 |
| ISSN | 1345-0948 |
| language | English |
| File Version | publisher |
| NAID | 120002313440 |
| Author | Okamoto, Yuko| |
|---|---|
| Published Date | 2007-03-23 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| Author | Yamashita, Toru| |
|---|---|
| Published Date | 2007-03-23 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| Author | Ohta, Yasuyuki| |
|---|---|
| Published Date | 2007-03-23 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| Author | Wu, Yumei| |
|---|---|
| Published Date | 2007-03-23 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| JaLCDOI | 10.18926/11730 |
|---|---|
| Title Alternative | Measurement of intracellular pH by flow cytometry using pH sensitive fluorescence dye, and influence of hyperthermia and amiloride derivatives on the intracellular pH |
| FullText URL | 006_001_005.pdf |
| Author | Asaumi, Jun-ichi| Kawasaki, Shoji| Kuroda, Masahiro| Takeda, Yoshihiro| Hiraki, Yoshio| |
| Abstract | エールリッヒ腹水癌細胞とそのアドリアマイシン耐性細胞において蛍光pH指示薬2'、7'-bis-(2-carboxyethyl) carboxyfluorescein] (BCECF) の蛍光量をフローサイトメトリーで測定することによって細胞内pHの検量曲線を作成することができた。このことより、これらの細胞においてBCECFの蛍光量で細胞内pHの変化を簡易に比較できることを示唆した。さらに、温熱、Na(+)/H(+) exchanger の阻害例であるアミロライド[3,5-diamino-6-chloro-N-(diaminomethylene) pyrazinecarboxamide]、およびアミロライド誘導隊MH-12-43[N-amidino-3-amino-6-chloro-5-(N-ethyliso-propylamino) pyrazinecarboxyamide] の細胞内pHへの影響をエールリッヒ腹水癌細胞で観察した。37℃では、0.5mMアミロライド、0.05mM MH-12-43により細胞内pHは減少し、42℃処理によりさらに減少した。42℃において、0.05mM MH-12-43による細胞内pHの減少は、0.5mMアミロライドによる減少より大きかった。 |
| Keywords | BCECF 細胞内pH (Intracellular pH) フローサイトメトリー (Flow Cytometry) アミロライド (Amiloride) MH-12-43 |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1996-02-29 |
| Volume | volume6 |
| Start Page | 1 |
| End Page | 5 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313855 |
| Author | Masuda, Kozo| |
|---|---|
| Published Date | 2007-03-23 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| Author | Sakoda, Yukimi| |
|---|---|
| Published Date | 2007-03-23 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| JaLCDOI | 10.18926/11716 |
|---|---|
| Title Alternative | Variations of parameters of bioelectrical impedance during upper limb movement |
| FullText URL | 010_1_015_022.pdf |
| Author | Nakamura, Takao| Yamamoto, Yoshitake| |
| Abstract | これまでに生体電気インピーダンスを用いて身体運動の解析を行ってきた。インピーダンスの変動原因は測定部位の等価断面積変化および貯血量変化と説明できるが，直接的に断面積変化や貯血量変化を測定することは困難であるため，その変動原因の定量的な検討は不十分であった。そこで，本研究では上肢運動に伴う生体電気インピーダンスの変動原因を生体の等価回路のパラメータの変動で説明する。インピーダンスのモデルはCole－Cole型であり，等価回路は，細胞外液抵抗Re，細胞内液抵抗Ri，分散の特性周波fm fm，緩和時間の分布の程度を表すα，βの独立した5パラメータで表現した。 上肢の肘関節および手関節の屈曲運動野に伴う前腕部および上腕部の電気インピーダンスを測定した。まず，電気インピーダンスの等価直列抵抗Rの変動率をパラメータの変動率で線形近似し，その信頼性と各パラメータ変動のRへの影響を確認した。Rへの影響はReとRiが支配的であるが，測定部位や運動の違いにより，それぞれの影響が異なることを示した。これは測定部位の筋活動状態の違いを表すものである。さらに，肘関節最大屈曲におけるRの変化にはfmの変化が大きく依存しており，上肢運動のインピーダンス変化には細胞膜の分極特性の変化も影響していることを明らかにした。本研究の結果は，運動強度の違いや疲労状態における身体運動評価に有意義である。 |
| Keywords | 生体電気インピーダンス (bioelectrical impedance) 等価回路 (equivalent circuit) Cole-Cole円弧 (Cole-Cole circular loci) 上肢運動 (upper limb movement) 4電極法 (four electrode method) |
| Publication Title | 岡山大学医学部保健学科紀要 |
| Published Date | 1999-12-20 |
| Volume | volume10 |
| Issue | issue1 |
| Start Page | 15 |
| End Page | 22 |
| ISSN | 1345-0948 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002314002 |
| Author | Sato, Yumiko| |
|---|---|
| Published Date | 2007-03-23 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| JaLCDOI | 10.18926/11693 |
|---|---|
| Title Alternative | Hyhrid vascular grafts and one of our approaches |
| FullText URL | 061_114_122.pdf |
| Author | Noishiki, Yasuharu| |
| Abstract | 人工血管により高度な機能をもたす目的で，ハイブリット化が今日進められるようになってきた。この背景について説明するとともに，我々の独自に行っているハイブリット型人工血管作成の方法およびその成果について報告する。その具体的方法としては以下の通りである。末梢静脈小片を勇刀にて細切したのち，約20ccの生理的食塩水に入れて静脈組織細切片浮遊液を作った。次に高有孔性人工血管の一端より吸引管を挿入し，これを作成した液に入れ，吸引によって組織片を外側から人工血管壁に絡ませた。次に新鮮な血液を注ぎ，組織片をさらに固着させた。成犬胸部下行大動脈へこのような処理をしたポリエステル布製人工血管を植え込んだところ，植え込み5日目に新生血管壁内部に無数の内皮細胞の増殖像がみられ，35日目の例では吻合部はもとより，人工血管の中央部ですら内皮細胞による完全な被覆を認めた。 以上のような方法で新生血管壁が容易に形成されることが判明したことより，今後，他の人工臓器分野でもハイブリット化が予測される。 |
| Keywords | 人工血管 (Vascular graft) ハイブリット (Hybrid) 新生内膜 (Neointima) 内皮細胞 (Endothelial cell) 自家移植 (Autologous transplantation) |
| Publication Title | 環境病態研報告 |
| Published Date | 1990-09 |
| Volume | volume61 |
| Start Page | 114 |
| End Page | 122 |
| ISSN | 0913-3771 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313776 |
| JaLCDOI | 10.18926/11687 |
|---|---|
| Title Alternative | New trend of the development of vascular prostheses |
| FullText URL | 061_098_113.pdf |
| Author | Noishiki, Yasuharu| |
| Abstract | 人工血管の開発研究における動向について，我々の行ってきた研究をまじえて世界の動きを解析した。近年の砺究の流れは過去1世紀にわたる試行錯誤の上にあって，さらに大きく揺れ動いている。人工血管の内面に必要な抗血栓性の獲得においても，設計者によって考えを異にし，米国では，人工心臓に用いる抗血栓性合性高分子材料をもってそれに当てようとしているものが多い。我々はあくまで宿主のもつ諸機能を引き出し，自然の動きの一つとしての治癒を無理なく導いて，内皮細胞によって内面を覆わせる方法を採用している。そのいくつかの例として，超極細ポリエステル繊維製人工血管，結合組織管，バイオマトリックス人工血管，ヘパリン化生体組織由来人工血管，成長できる人工血管などについて解説した。 |
| Keywords | 人工血管 (Vascular prosthesis) 新生内膜 (Neointima) 内皮細胞 (Endothelial cells) 抗血栓性 (Antithrombogenicity) |
| Publication Title | 環境病態研報告 |
| Published Date | 1990-09 |
| Volume | volume61 |
| Start Page | 98 |
| End Page | 113 |
| ISSN | 0913-3771 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313859 |
