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JaLCDOI 10.18926/11875
Title Alternative Ferrous Iron-induced Lipid Peroxidation in Cell Culture: Effects of Ferrous and Ferric Irons on Cell Survival and Serum Lipid Peroxide
FullText URL 004_061_068.pdf
Author Yamamoto, Goki|
Abstract Cell killing activities of ferrous and ferric irons (Fe(2+), Fe(3+)) in cultured 3T3 cells were investigated by the colony forming method in the culture medium containing 10% calf serum. In the colony forming method, small isolated colonies are seen in the dish which are the resulted of individual cells having undergone a series of cell divisions. Survival curve for Fe(2+)-induced loss of reproductive capacity (cell death) in 3T3 cells, showed a shoulder at low concentrations and only became exponential at high concentrations. Cells affected at 0.05mM Fe(2+) underwent apparently normal cell divisions, but at a much slower rate than the control. Survival rate at 0.5mM Fe(2+), which iron was added after seeding cells (added group), showed 0.75% but that at 0.5mM Fe(3+) did 19.3%. From the determination of Fe(2+) content carried out by the colorimetric method used N-PSAP, the oxidation of Fe(2+) was seen rapidly after adding Fe(2+) into the culture medium. In the cases of cells seeded 3 hours after adding Fe(2+) or Fe(3+) (preadded group), the survival rates showed much lower than those of the added groups, that is 55% at 0.5mM Fe(2+) and 89% at 0.5mM Fe(3+). The facts suggest that the radical reactions iniciated by the oxidation of Fe(2+) under the presence of cells, occured just after adding Fe(2+), play a crucial role in the damages on the adhesion of cells onto the dish and the following proliferation of cells. Serum lipid peroxides, as thiobarbituric acid reactive substances (TBA value), of the final cultured medium at colony counting time were measured. In the Fe(2+) added group, the TBA values more increased in proportion to the added concentrations of Fe(2+), whereas in the Fe(3+) added group, the increments of TBA values were very slightly and not to be depended on the concentrations. Furthermore, the TBA values of the Fe(2+) or Fe(3+) preadded groups were estimated as well as those of the added groups, respectively. The facts suggest that serum lipid peroxides induced by Fe(2+) have little effect on the proliferation capacity of cells in the level of cell culture.
Keywords Cell Survival Ferrous and Ferric Irons Serum Lipid Peroxide
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1994-01-31
Volume volume4
Start Page 61
End Page 68
ISSN 0917-4494
language Japanese
File Version publisher
NAID 120002313910
JaLCDOI 10.18926/11830
Title Alternative Ferrous Iron-induced Lipid Peroxidation in Cell Culture: preliminary experiments
FullText URL 003_049_053.pdf
Author Yamamoto, Goki|
Abstract To study the effect of Fe(2+) on the cell culture, preliminary experiments related to the lipid peroxidations of 3T3 cells and calf serum were carried out. Amonuts of lipid peroxides, as thiobarbituric acid (TBA)-reactive substances, were measured by means of the TBA color reaction under the acidic conditions by acetic acid, and were expressed as TBA value. The TBA value of calf serum in the phosphate buffered saline (PBS) with ethyrendiaminetetraacetate (EDTA) was lower than that of the control. The value in the presence of Fe(2+) showed significantly high level, but it was almost prevented by the addition of EDTA. Besides, Fe(2+)-induced lipid peroxides of serum in the PBS during the incubation at 37℃ were capable to measure by the application of EDTA. The TBA values of trichloroacetic acid (TCA) precipitates isolated from serum in the PBS and the culture medium were lower than that of control serum, but no difference was observed between the both TCA precipitates. The results suggested that the impurities in the culture medium were removed by TCA precipitation. Effects of Fe(2+) and EDTA on the TBA value of TCA precipitates were observed similarly to the case of control serum. Fe(2+)-induced lipid peroxidation of the cells in the PBS, which the peroxidation was stopped by the addition of EDTA, was observed, and it was accompanied with the lag time of induction period. The TBA value of the cells was more prevented by EDTA. The method in this paper may not be capable for the quantitative measurements of lipid peroxides in cells or serum, but is applicapable to measure the changes of relative amounts of lipid peroxides.
Keywords Lipid Peroxidation Ferrous Iron Cell Culture
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1993-01-31
Volume volume3
Start Page 49
End Page 53
ISSN 0917-4494
language Japanese
File Version publisher
NAID 120002313457
JaLCDOI 10.18926/11828
Title Alternative Measurement of Fe(2+)-induced Lipid Peroxidation of Mitochondria used Thiobarbituric Acid Method under the Acetic Acid Conditions
FullText URL 003_043_047.pdf
Author Yamamoto, Goki|
Abstract Mitochondrial lipid peroxidations were usually detected by the thiobarbituric acid (TBA) color reaction under the acidic conditions with hydrochloric and trichloroacetic acids. The time response curve of Fe(2+)-induced lipid peroxidation, accompanied the lag time of induction period, of mouse liver mitochondria was measured with detection of the produced lipid peroxides by the TBA method under the acidic conditions with acetic acid. Fe(2+)-induced lipid peroxidation was terminated by the addition of ethylenediaminetetraacetate (EDTA). The lag time of the peroxidation detected by TBA method in the acetic acid conditions was obtained as well as that detected in the hydrochloric and trichloroacetic acids conditions. The fact indicates that Fe(2+)-induced lipid peroxidation of mitochondria and its lipid peroxides are able to be measured by the TBA color reaction in the acetic acid conditions with EDTA.
Keywords Mitochondrial Lipid Peroxidation Thiobarbituric Acid Ferrous Ion
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1993-01-31
Volume volume3
Start Page 43
End Page 47
ISSN 0917-4494
language Japanese
File Version publisher
NAID 120002313733
JaLCDOI 10.18926/11801
Title Alternative Measurement of Serum Lipid Peroxides used Thiobarbituric Acid Method: Colorimetric and Fluorometric Assays and Effect of Ferrous Ion
FullText URL 002_051_061.pdf
Author Yamamoto, Goki|
Abstract Serum lipid peroxides were measured by a thiobarbituric acid (TBA) method. TBA color reactions carried out under the conditions with acetic acid (TBA-AA) or hydrochloric acid (TBA-HCl) systems, and then TBA value was detected by colorimetric ([OD]) and fluorometric ([FR]) measurements. Effects of ferrous ion, ferric ion and EDTA on the both systems were examined. Equal TBA value dependent upon concentrations of MDA (tetraethoxypropane) were obtained on both systems detected by [OD], but the relative fluorescence of TBA-AA system was higher than that of TBA-HCl system. Fluorometric determination was suitable for low TBA value, and colorimetric one was for high TBA value. Good correlation between TBA[OD] values and TBA[FR] values of serum from patients was estimated, but it was suggested that effects of serum substance were differ on the both systems. Effects of iron on TBA-AA and TBA-HCl systems were different significantly, particularly high Fe-TBA values obtained from TBA-AA system in the presence of ferrous ion. The fact suggests that serum lipid peroxidation is induced by ferrous ion during a heating of TBA color reaction. Same effect of ferric ion in TBA-AA system was observed. The increment of TBA values by ferrous and ferric ions was inhibited by a addition of EDTA. It was considered that the difference effect of iron on the both systems was due to pH and acetic acid concentration. The relationship of TBA values and Fe-TBA values of serum from patients was no correlated, and the various inhibition ratios of EDTA for the TBA value was calculated. It is suggested that Fe-TBA value is possible to detemine the qualitative difference of serum lipid peroxides.
Keywords Serum Lipid Peroxides Thiobarbituric Acid Ferrous Ion
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1992-02-05
Volume volume2
Start Page 51
End Page 61
ISSN 0917-4494
language Japanese
File Version publisher
NAID 120002313802
JaLCDOI 10.18926/11757
Title Alternative Lipid Peroxides (TBA reactive substances)and Fatty Acid Compositions in Mouse Serum with Whole-body Irradiation and in Tumor-bearing Mouse Serum with Local Irradiation
FullText URL 001_029_037.pdf
Author Yamamoto, Goki|
Abstract Effects of irradiation on lipid peroxides of mouse serum, Ehrlich solid tumor-bearing mouse serum, which tomor cells were transplanted to the leg, and its tumor tissue were studied by a thiobarbituric acid (TBA) color reaction in the acetic acid condition with (Fe-TBA value) or without (TBA value) ferrous ammonium sulfate. TBA reactive substances were caluculated into the amount of malondialdehyde. Besides, fatty acid and lipid compositions were analyzed as a substrates. Irradiated samples were isolated at 3 days after irradiations. The TBA value of normal mouse serum was expressed 17nmoles/ml of serum, and Fe-TBA value gave 2.4-fold of the TBA value. Although an increase TBA value was not observed by whole-body irradiation, a significant increase of Fe-TBA value was measured, indicating 2.5-fold at 10Gy exposure compared with the normal Fe-TBA value, and 5.5-fold with the TBA value. The TBA value of tumor-bearing mouse serum was 14nmoles/ml as a low rate to that of normal serum, but the Fe-TBA value gave same magnification of that of normal one. The TBA value of tumor-bearing mouse serum was not changed by the local irradiation to the tumor region of leg, but the Fe-TBA value was increased 2.8 and 4.4 times at 10Gy and 20Gy exposures, respectively, comparing with the non-irradiated one, and 7.7 and 10.5 times with the TBA value, respectively. The TBA value of solid tumor homogenate exhibited 1.16nmoles/mg of protein, and the Fe-TBA value, pointed out 5 times as much as the TBA value. Both values slightly increased by the irradiation. These facts suggest that a shift of TBA value of serum hard to get, but Fe-TBA value of serum distinctly increases by the irradiation. The fatty acid composition of mouse serum lipids showed an increment of relative percentages of linoleic and arachidonic acids by the whole-body irradiation. The relative percentage of fatty acid compositon of lipids from solid tumor-bearing mouse serum was similar tendency to that of normal one, and the local exposure to the tumor part was not affected the each percentage. The percentage of high unsaturated fatty acid of tumor lipids similar to that of serum lipids, and a decrease of the percentage of arachidonic acid was accounted by the irradiation. In relative percentages of lipid compositions, the percentage of choleste rolester of serum lipids increased by the whole-body irradiation, and that of phospholipid fraction was not changed. In the case of tumor-bearing mouse serum lipids, an increment of the percentage of cholesterolester was obtained as compared with that of normal serum lipids, and this percentage decreased by the local irradiation to tumor part with an increment of the percentage of phospholipid. The percentage of phospholipid in tumor lipids had decreased by the irradiation. The facts suggest that a cause of the increased Fe-TBA value of tumor-bearing mouse serum by the irradiation to tumor is due to the release of peroxidizable phospholipid into serum from the damaged menbranes of tumor cells at large doses to exposure.
Keywords Lipid peroxides Irradiation Mouse serum Tumor-bearing mouse serum Fatty acid composition
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1991-03-25
Volume volume1
Start Page 29
End Page 37
ISSN 0917-4494
language Japanese
File Version publisher
NAID 120002313903
JaLCDOI 10.18926/11726
Title Alternative 生体電気信号の計測システムへの電力線からの誘導障害に関するインターフェイスモデル
FullText URL 010_2_107_115.pdf
Author Adli| Yamamoto, Yoshitake| Nakamura, Takao|
Abstract A modelling of interface construction relating to power-line interference phenomenon was described. Source of interference was displacement currents which flowed from AC power-line configuration (APC) to a model. The interference depends on some factors: distance between the APC and a model ; length of unshielded leads ; position of the APC in vertical or horizontal direction ; and skin-electrode impedances which were balance or unbalanced. The position of APC and skin-electrode impedances were important in contributing of the interference. The interference was still large even though skin-electrode impedances were balance. This was caused by the disagreement in two displacement currents. It was overcame by adjustment of APC in vertical direction. As a result, total interference would be very small or zero. The result shows that interference in ECG signal recording can be eliminated to about 10 μV. Although interference of power-lines is a complicated phenomenon, the problem is easier to understand by using this modelling.
Keywords AC power-line configuration (APC) (電力線配置) biopotential signal (生体電気信号) displacement current (変位電流) physical model of interference (モデル) power-line interference (誘導障害)
Publication Title 岡山大学医学部保健学科紀要
Published Date 2000-03-24
Volume volume10
Issue issue2
Start Page 107
End Page 115
ISSN 1345-0948
language English
File Version publisher
NAID 120002313382
JaLCDOI 10.18926/11716
Title Alternative Variations of parameters of bioelectrical impedance during upper limb movement
FullText URL 010_1_015_022.pdf
Author Nakamura, Takao| Yamamoto, Yoshitake|
Abstract これまでに生体電気インピーダンスを用いて身体運動の解析を行ってきた。インピーダンスの変動原因は測定部位の等価断面積変化および貯血量変化と説明できるが,直接的に断面積変化や貯血量変化を測定することは困難であるため,その変動原因の定量的な検討は不十分であった。そこで,本研究では上肢運動に伴う生体電気インピーダンスの変動原因を生体の等価回路のパラメータの変動で説明する。インピーダンスのモデルはCole-Cole型であり,等価回路は,細胞外液抵抗Re,細胞内液抵抗Ri,分散の特性周波fm fm,緩和時間の分布の程度を表すα,βの独立した5パラメータで表現した。 上肢の肘関節および手関節の屈曲運動野に伴う前腕部および上腕部の電気インピーダンスを測定した。まず,電気インピーダンスの等価直列抵抗Rの変動率をパラメータの変動率で線形近似し,その信頼性と各パラメータ変動のRへの影響を確認した。Rへの影響はReとRiが支配的であるが,測定部位や運動の違いにより,それぞれの影響が異なることを示した。これは測定部位の筋活動状態の違いを表すものである。さらに,肘関節最大屈曲におけるRの変化にはfmの変化が大きく依存しており,上肢運動のインピーダンス変化には細胞膜の分極特性の変化も影響していることを明らかにした。本研究の結果は,運動強度の違いや疲労状態における身体運動評価に有意義である。
Keywords 生体電気インピーダンス (bioelectrical impedance) 等価回路 (equivalent circuit) Cole-Cole円弧 (Cole-Cole circular loci) 上肢運動 (upper limb movement) 4電極法 (four electrode method)
Publication Title 岡山大学医学部保健学科紀要
Published Date 1999-12-20
Volume volume10
Issue issue1
Start Page 15
End Page 22
ISSN 1345-0948
language Japanese
File Version publisher
NAID 120002314002
JaLCDOI 10.18926/11688
Title Alternative Relationships between electrical impedance and light transmission of flowing blood
FullText URL 011_2_065_070.pdf
Author Nakamura, Takao| Yamamoto, Yoshitake|
Abstract 流動する血液についての力学的,光学的および電気的な特性についての研究が数多く報告されている。本研究では,狭窄がある人工血管を流れる血液の電気インピーダンス(周波数10kHz)を近赤外光(波長940nm)の透過度と同時に測定し,血液の電気インピーダンスの特性について検討を行った。内径2mmのアクリル製の血管モデルを作製し,その内部に断面積の小さい狭窄部位を1ヵ所設置し,ヒトの血液(Hct40%)を流した。定常流においては血流速度の増加に伴いインピーダンスの相対変化量および透過光強度ともに減少した。しかし,狭窄部に近い部位では,インピーダンスと透過光強度の変化パターンに違いがみられた。インピーダンスおよび透過光強度は流動する血球の動きや配向等を反映していると考えられた。
Keywords 流動血液 (flowing blood) 生体電気インピーダンス (bioelectrical impedance) 定常流 (steady flow) 拍動流 (pulsatile flow) 透過光強度 (light transmission)
Publication Title 岡山大学医学部保健学科紀要
Published Date 2001-03-24
Volume volume11
Issue issue2
Start Page 65
End Page 70
ISSN 1345-0948
language Japanese
File Version publisher
NAID 120002314001
JaLCDOI 10.18926/fest/11622
FullText URL 001_121_133.pdf
Author Maeno, Shiro| Yamamoto, Tetsuo| Nago, Hiroshi|
Abstract In this paper the theoretical procedure to analyze the dynamic behavior of highly saturated sand bed around a cylindrical block under the cyclic loading of water pressure is developed. The fundamental equations were derived for the axially symmetric coordinates. Then, the finite element equations were developed to solve these fundamental equations numerically. Finally, the numerical method was verified by experiments.
Publication Title 岡山大学環境理工学部研究報告
Published Date 1996-03
Volume volume1
Issue issue1
Start Page 121
End Page 133
ISSN 1341-9099
language English
File Version publisher
NAID 120002313416
JaLCDOI 10.18926/11418
Title Alternative Clinical Evaluation and Assessment in School Setting
FullText URL 003_155_166.pdf
Author Yamamoto, Tsutomu| Tsukamoto, Chiaki| Nishiyama, Hisako| Akazawa, Hiroshi|
Abstract 以下の報告は、平成14年8月26日に開催された岡山大学教育学部附属教育実践総合センター研修講座の教育臨床部門の分科会「シンポジウム:教育臨床における評価・見立てについて」での報告内容をもとに、発表者がそれぞれに再構成して書き下ろしたものである。シンポジウムのコーディネーターは塚原千秋が行った。そして、まず心理臨床の視点から山本が教育臨床の見立てとは何かについて模索した見解を報告した。続いて専任スクールカウンセラーの立場から西山が米国のスクールカウンセリングをモデルにした評価の仕方を詳しい報告にした。最後に学校でのブリーフセラピーの可能性を探る教師の視点から赤沢が実践的な方法と認識を報告した。
Keywords 教育臨床 (Clinical Judgement) 評価・見立て (Assessment) 教育相談体制 (School Counseling)
Publication Title 岡山大学教育実践総合センター紀要
Published Date 2003
Volume volume3
Issue issue1
Start Page 155
End Page 166
ISSN 1346-3705
language Japanese
File Version publisher
NAID 120002313687
JaLCDOI 10.18926/11403
Title Alternative "School of theTANBO"
FullText URL 003_047_056.pdf
Author Yamamoto, Kazufumi|
Abstract 「田んぼの学校」は平成10年度の国土、文部、農水の3省庁合同で提唱された、環境教育の取組のことである。今年度、岡山県農林水産部耕地課が事業主体となり、そのモデル推進事業を岡山市福谷小学校で実施中。企画とその運営には福谷小PTAとボランティア団体から実行委員を構成してあたっている。私はその常任委員を務めているが、PTAと地域各位の多大な支援のもと年間12回もの多彩な開校が企画され現在までに9回実施、延べ551名の参加者を得ている。それぞれの企画は子供達と実行委員の希望で立案されたが、単なるイベントではなく参加者の自主的な活動によって成り立っている。親と子、そして地域の人々が一体となって架空の学校を育て上げており、この事例は今後の学校のあり方を考える上で大変興味深くかつ有益な活動である。
Keywords 田んぼの学校 (School of "TANBO") 環境教育 (Environment education) 農業教育 (Agriculture education) 地域活動 (Community sctivities) ボランティア (Volunteer)
Publication Title 岡山大学教育実践総合センター紀要
Published Date 2003
Volume volume3
Issue issue1
Start Page 47
End Page 56
ISSN 1346-3705
language Japanese
File Version publisher
NAID 120002313705
JaLCDOI 10.18926/11402
Title Alternative A Guideline for Heiping a Child of School Refusal and his/her Parents
FullText URL 005_131_137.pdf
Author Yamamoto, Tsutomu|
Abstract 教育臨床家が不登校の子どもの支援を行う際に「臨床的判断」の参照枠となるガイドライン試案を作成することが本論文の目的である。不登校の多様な状態像を見立てる指針として、a)登校状況からみた5型類、b)不登校の6段階の経過、c)7つの原因論、を提示した。その上で教育臨床家による不登校支援の原則を12項目にわたって列記した。教育臨床的な支援12項目は不登校の臨床実践から抽出された経験則が中心である。不登校の子どもの多様な歩みを保障する一方で、ガイドラインを参照しながら社会的自立へと向かえるように周囲が連携して支援していくことが求められる。
Keywords 不登校 (School refusal) 教育臨床家 見立て(見極め) (Assessment) 支援 (Support) ガイドライン (Guideline)
Publication Title 岡山大学教育実践総合センター紀要
Published Date 2005
Volume volume5
Issue issue1
Start Page 131
End Page 137
ISSN 1346-3705
language Japanese
File Version publisher
NAID 120002313504
JaLCDOI 10.18926/11387
Title Alternative The Background and Trend of Practical Peer Support and Peer-assisted Activities :the Beginning to Current Tendency of Peer Support and Peer-assisted Activities
FullText URL 002_081_093.pdf
Author Nishiyama, Hisako| Yamamoto, Tsutomu|
Abstract 私たちの社会では、習慣的に仲間支援の手法が用いられてきたが、近年、「ピアサポート」が学校教育現場に実践的に導入され、欧米で開発されてきた技法なども紹介されて仲間関係を積極的に活用しようという動きがある。福祉の領域などでは、より積極的に仲間支援が行われ、歴史的に見ても教育・福祉等の分野で活用してきた経緯がある。そうした支援は、相談活動、葛藤調停、仲間づくり、アシスタント、学習支援、指導、助言、グループリーダーの7項目に分類される。学校で生徒がもつ仲間支援の力が十分活用するようにプログラムが構成されるとき、生徒らにとって有効なピアサポート活動が実施できる。
Keywords ピアサポート (peer-support) 仲間 (peer) ピアカウンセリング (peer-counseling) ソーシャルサポート (social support)
Publication Title 岡山大学教育実践総合センター紀要
Published Date 2002
Volume volume2
Issue issue1
Start Page 81
End Page 93
ISSN 1346-3705
language Japanese
File Version publisher
NAID 120002313981
JaLCDOI 10.18926/11377
Title Alternative The Qualitative Analysis of Effective Work of Mourning by Victims' Family
FullText URL 004_137_145.pdf
Author Yamamoto, Tsutomu| Hamasaki, Midori| Tamai, Chisato| Yamasaki, Fumiko|
Abstract 平成13年6月8日に附属池田小学校で発生した児童殺傷事件の被害者遺族の一人である本郷由美子さんの手記『虹とひまわりの娘』を分析素材として、どんな悲嘆の対処法や社会的サポートがモーニングワークの促進に有効であるのかを探索的に探ることを研究の目的とした。加えて、「死者を殺す」というラガーシュ仮説の妥当性に関する若干の検討を行った。その結果、モーニングワークを通して親子の「絆」の結び直しがはかられ、娘に対する「愛の物語」が生成されていった過程が明らかになった。
Keywords 被害者家族 (the bereaved) モーニングワーク (mourning work) 物語 (story-making) 社会的サポート (social support) ラガーシュ仮説 (Lagache hypothesis)
Publication Title 岡山大学教育実践総合センター紀要
Published Date 2004
Volume volume4
Issue issue1
Start Page 137
End Page 145
ISSN 1346-3705
language Japanese
File Version publisher
NAID 120002313879
JaLCDOI 10.18926/11376
FullText URL 004_127_136.pdf
Author Yamamoto, Kazufumi|
Abstract Cabriole Legsは日本では、”猫足”と訳され、西洋クラシック家具の部分的形態として捉えられるにすぎない。したがってこの脚についての文化的・歴史的記述や、加工方法やフォルムについて述べられたことはない。今回様々な情報源を探り、歴史や形態のパターン、また具体的なサイズ、加工方法などを明らかにすることで実践研究への序章とする。
Keywords Cabriole Legs 猫足 (Cat foot) QueenAnne Chippendale ロココ (Rococo) 18世紀 (18th century)
Publication Title 岡山大学教育実践総合センター紀要
Published Date 2004
Volume volume4
Issue issue1
Start Page 127
End Page 136
ISSN 1346-3705
language Japanese
File Version publisher
NAID 120002313458
JaLCDOI 10.18926/11352
Title Alternative About the lifelong education at the university : Through the practice of "woodwork seminar"
FullText URL 001_021_030.pdf
Author Yamamoto, Kazufumi|
Abstract 生涯教育の体制は発展途上と言って良いが、その中のものづくり教育について考察した。特に木工分野は学習の機会が少なく、内容的にも問題を感じている。今年、実践した社会人木工セミナーを例に学習支援の必要性と、それを前提とした題材、また同時に結果だけを求める学習者の意識的な問題が明らかになった。この講座の設定方法を巡って問題を感じたため、大学における社会人教育の窓口について考察を行った。文部省生涯学習審議会はその拡充を求めており、特に公開講座を授業科目で行うことは有効である。これに半歩踏み出したかたちで木工講座の具体案を提案した。
Keywords 社会人教育 生涯教育 (lifelong education) 木工講座 (woodwork lecture) 公開講座 (open class)
Publication Title 岡山大学教育実践総合センター紀要
Published Date 2001
Volume volume1
Issue issue1
Start Page 21
End Page 30
ISSN 1346-3705
language Japanese
File Version publisher
NAID 120002313707
Title Alternative タバコ懸濁培養細胞におけるアルミニウムの二価鉄、銅およびカドミウム毒性に対する影響
FullText URL 002_002_181_190.pdf
Author Yamamoto, Yoko| Chang, Yi-Chieh| Ono, Kanji| Matsumoto, Hideaki|
Abstract The effects of aluminum (Al) on the cytotoxicity of ferrous iron (Fe(Ⅱ)), copper(Cu) and cadmium(Cd) were studied. Log-phase cells were treated with either FeSO4,CuSO4, or CdCl2 in the presence or absence of AlCl3(120μM) for 18h at pH 4.0. After the treatment, the viability was determined as relative growth of the metal-treated cells to the untreated control cells during the post-treated culture. A single treated with either Al, Fe(Ⅱ) or Cd did not inhibit the growth at the metal concerntrations up to 300 μM, 200 μM and 500 μM, respectively, whereas the growth was markedly inhibited at 15 μM Cu. Thus,the cells were relatively insensitive to Al, Fe(Ⅱ) and Cd and sensitive to Cu. When cells were treated with both Fe(Ⅱ)(120 μM)and Al(120μM), the growth was significantly inhibited and the cellular contents of both Al and Fe increased synergistically. After the treatment with Cu(0 to 10 μM) with or without Al, the cells grew more vigorously when they were treated in the presence of Al, althrouh the Cu content of the cells were not alterd by Al. The presence of Al during the treatmemt with Cd(0 to 2 μM) had no effect on the degree of growth inhibition by Cd. Thus, Al interacts with the toxicity of Fe(Ⅱ), Cu and Cd in different manners; synergistic with Fe(Ⅱ), antagonistic with Cu and apparently no effeco on Cd.
Keywords Aluminum Antagonistic Nicotiana tabacum Suspension culture Synergistic
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1994
Volume volume2
Issue issue2
Start Page 181
End Page 190
ISSN 0916-930X
language English
File Version publisher
Author Yamamoto, Mikihiro|
Published Date 1997-02
Publication Title 岡山大学農学部学術報告
Volume volume86
Issue issue1
Content Type Departmental Bulletin Paper
Author Matsubara, Sachiko| Yamamoto, Masatoyo| Jo, Man Hyun| Murakami, Kenji|
Published Date 1998-02
Publication Title 岡山大学農学部学術報告
Volume volume87
Issue issue1
Content Type Departmental Bulletin Paper