免疫反応における気管支喘息患者好塩基球の生態観察に関する研究 第二編抗免疫グロブリンの好塩基球形態に及ぼす影響

Morphologic changes in vitro of basophils from the peripheral blood of patients with atopic bronchial asthma (serum IgE more than 1000 u/ml) and intractable bronchial asthma (serum IgE less than 100 u/ml) were studied by addition of anti-IgE or anti-IgG. In supravital observation by differential-inteference microscopy, basophils when exposed to anti-immunoglobulin moved rapidly and changed morphologically to a pear-shape and next to a hand-shape and the granules were degranulated from the tip of the cytoplasmic processus in a manner of exocytosis. In the observation by phase contrast microscopy, intracellular changes of granules and vacuolation were observed clearly, but ejection of granules were not observed by this method.