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Title Alternative Sexuality in plants, unveiled from genome evolution
FullText URL srfa_109_037_040.pdf
Author Akagi, Takashi|
Abstract Sexual polymorphism, a main strategy to maintain genetic diversity within a species, has long been a major focus in biology. Notwithstanding, in plants, evolution of sexual systems and mechanisms underlying these transitions have been little unveiled. We have elucidated the molecular mechanism of sex determination in persimmons (Diospyros spp.), where the Y-encoded smRNA gene OGI can repress the female-determining gene MeGI, and also in kiwifruits (Actinidia spp.), where the Y-encoded two sex determinants, Shy Girl and Friendly Boy, control gynoecium and androecium development, respectively. Although the molecular functions of these determinants are distinct, they have common evolutionary scenarios involving transitions of sexual systems. In persimmon, a recent genome triplication (hexaploidization) in cultivated persimmon (D. kaki) derived “flexible” sexuality via establishing epigenetic layers on the two sex determinants. On the other hand, an ancient Diospyros-specific paleo-genome duplication (paleo-tetraploidization) enabled neofunctionalization in the proto-MeGI, via positive selection, to establish a new function as a sex determinant. In kiwifruit, one of the two sex determinants, Shy Girl, was derived from neofunctionalization via Actinidia-specific duplication event. These findings exemplify how plant-specific numerous duplication events can drive flexible genetic material whose variation can be selected for development of new sexual systems.
Keywords Sex determination Sex chromosome Polyploidization Genome evolution Tree crops
Publication Title Scientific Reports of the Faculty of Agriculture, Okayama University
Published Date 2020-02-01
Volume volume109
Start Page 37
End Page 40
ISSN 2186-7755
language Japanese
File Version publisher
Title Alternative Development of Effective Biological Treatment Process for Acid Mine Drainage
FullText URL srfa_109_029_036.pdf
Author Kamimura, Kazuo| Kanao, Tadayoshi|
Abstract Acid mine drainage (AMD) is acidic and generally enriched with iron, aluminum, sulfate and heavy metals, such as lead and cadmium. AMD is a growing problem of emerging concern that cause detrimental effects to the environment and living organisms. Yanahara mine in Misaki Town, Okayama, Japan, had mainly produced pyrite for sulfuric acid manufacture. Although it was closed in 1991, AMD is being generated from the mine now. A passive treatment based on the biological oxidation of ferrous iron is a promising strategy for AMD remediation. AMD from Yanahara mine is treated in a plant using iron-oxidizing bacteria, Acidithiobacillus ferrooxidans and Ferrovum spp. The AMD generation continues for several centuries with dramatic consequences on the receiving environments. Therefore, the development of sustainable and cost effective treatment process is required. A development of the effective biological treatment process with an iron oxidation reactor operated at pH 3.5 is described in this report. Economic aspects are also discussed.
Keywords acid mine drainage bioremediation iron-oxidizing bacteria microbial community Acidithiobacillus ferrooxidans
Publication Title Scientific Reports of the Faculty of Agriculture, Okayama University
Published Date 2020-02-01
Volume volume109
Start Page 29
End Page 36
ISSN 2186-7755
language Japanese
File Version publisher
FullText URL srfa_109_021_027.pdf
Author Annah Khatenje Indeche| Yoshida, Yuichi| Miyaji, Daisuke| Goto, Tanjuro| Yasuba, Ken-ichiro| Tanaka, Yoshiyuki|
Abstract The objectives of this study were to (i) determine the optimum number of whole leaves to retain on a tomato plant for effective blossom-end rot (BER) management and (ii) explore the relationship between shoot calcium (Ca) and fruit Ca in non-defoliated plants in two different sized fruit cultivars, a large-fruited cultivar ‘Momotaro fight’ and a medium-fruited cultivar ‘Cindy sweet’. Treatments involved maintaining 18, 15 and 12 leaves on a plant. All lateral shoots were removed regularly throughout the growing period except the shoot closest to the flowering truss in the 18-leaf treatment. At the length of 10cm, these shoots were sampled for real time Ca determination using a hand held Ca2+ meter. In the plants defoliated to 18 leaves, BER was higher in ‘Momotaro fight’ at 10% compared to 2% in ‘Cindy sweet’. Fruit growth rate was significantly increased by defoliation in ‘Momotaro fight’, however no significant difference was observed among treatments in ‘Cindy sweet’. Defoliating to 12 leaves increased daily Ca transport rate by 59% and 37% in ‘Momotaro fight’ and ‘Cindy sweet’, respectively. Defoliating to 12 leaves increased the water-soluble Ca concentration in the distal part of fruit by 34% and 14% in ‘Momotaro fight’ and ‘Cindy sweet’, respectively. In the plants defoliated to 18 leaves where only old yellowish leaves were removed, a significant steady decrease was observed in the concentration of water soluble Ca in the distal part of fruit with increase in truss order. There was a significant linear relationship between water-soluble Ca concentration in the distal part of the fruit and Ca concentration in the lateral shoot of plants defoliated to 18 leaves. We conclude that under moderate water stress by root zone restriction and also certain other BER inductive conditions, defoliation to 12–15 leaves on a tomato plant should be a promising approach for decreasing BER incidence in susceptible large fruit cultivars.
Keywords BER management defoliation water-soluble Ca lateral shoot Ca root zone restriction
Publication Title Scientific Reports of the Faculty of Agriculture, Okayama University
Published Date 2020-02-01
Volume volume109
Start Page 21
End Page 27
ISSN 2186-7755
language English
File Version publisher
FullText URL srfa_109_013_020.pdf
Author Nguyen Quang Co| Nguyen Thi Thanh Hien| Saitoh, Kuniyuki|
Abstract The field study was conducted in Nam Dong district, Thua Thien Hue province, Vietnam during May 2019 to observe the effects of SRI method on the rice yield and eradicate the factor affecting the yield. The study was carried out to evaluate the agronomic characteristics of rice, fresh and dry matter production, soil characteristics with a focus group discussion regarding cultivation by SRI and non-SRI methods. The soil analyses using standard measurements showed quite similar soil fertility in both SRI and non-SRI methods, while rice plants showed good agronomic performances and fresh and dry matter production in SRI method. At harvesting time, the yield and yield potentials of rice were greater in SRI than that in non-SRI methods. Focus group discussion found some factors that limited SRI development in Nam Dong district.
Keywords Actual yield Focus group discussion Filled spikelets SRI Weeding
Publication Title Scientific Reports of the Faculty of Agriculture, Okayama University
Published Date 2020-02-01
Volume volume109
Start Page 13
End Page 20
ISSN 2186-7755
language English
File Version publisher
FullText URL JACS7_1_90.pdf
Author Hayakawa, Satoshi| Okamoto, Keigo| Yoshioka, Tomohiko|
Keywords Titanium substrate apatite deposition simulated body fluid parallel alignment titania layer
Published Date 2019-02-11
Publication Title Journal of Asian Ceramic Societies
Volume volume7
Issue issue1
Publisher Taylor & Francis
Start Page 90
End Page 100
ISSN 2187-0764
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © 2019 The Author(s).
File Version publisher
DOI 10.1080/21870764.2019.1572690
Web of Science KeyUT 000459883500009
Related Url isVersionOf https://doi.org/10.1080/21870764.2019.1572690
FullText URL JACS20191699230.pdf
Author Liu, Xingzhu| Yoshioka, Tomohiko| Hayakawa, Satoshi|
Keywords Rutile nanorod arrays apatite rod density
Published Date 2019-12-02
Publication Title Journal of Asian Ceramic Societies
Publisher Taylor & Francis
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © 2019 The Author(s).
File Version publisher
DOI 10.1080/21870764.2019.1699230
Web of Science KeyUT 000500730700001
Related Url isVersionOf https://doi.org/10.1080/21870764.2019.1699230
FullText URL AEZ54_4_389.pdf Fig.pdf
Author Sakakibara, Akira| Takebe, So| Ide, Toru| Hayakawa, Tohru|
Keywords Bacillus thuringiensis Cry46Ab toxin Planar lipid bilayer Single-channel analysis Calcein release assay
Published Date 2019-08-16
Publication Title Applied Entomology and Zoology
Volume volume54
Issue issue4
Publisher Springer
Start Page 389
End Page 398
ISSN 0003-6862
NCID AA00543238
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
File Version author
DOI 10.1007/s13355-019-00635-z
Web of Science KeyUT 000503219100007
Related Url isVersionOf https://doi.org/10.1007/s13355-019-00635-z
Title Alternative Mathematical analysis of copy number variation of 2 μ-based plasmids in yeast cells
FullText URL srfa_109_007_011.pdf
Author Saeki, Nozomu| Moriya, Hisao|
Abstract  Plasmids with the 2 μ plasmid origin are commonly-used in the genetic engineering of the budding yeast Saccharomyces cerevisiae. Intracellular copy numbers of 2 μ plasmids are different depending on the genes inserted into the plasmids. This difference is thought to occur from the difference in the growth efficiency (fitness) produced by the positive- and negative-selection biases of genes inserted in the plasmid. In this study, we made a mathematical model based on this assumption. Computational simulations of the model validated that copy numbers of the plasmids are rapidly settled depending on the fitness created by the gene on the plasmid. The copy number of a plasmid only contains a bias to keep the plasmid in a single copy became average 20copies per cell when the plasmid is randomly distributed, suggesting that no positive distribution mechanism is required for a plasmid to become multicopy.
Keywords yeast 2 μ plasmid mathematical model
Publication Title Scientific Reports of the Faculty of Agriculture, Okayama University
Published Date 2020-02-01
Volume volume109
Start Page 7
End Page 11
ISSN 2186-7755
language Japanese
File Version publisher
Title Alternative Recombinant expression and characterization of quinone-containing novel glycine oxidase from Marinomonas mediterranea
FullText URL srfa_109_001_006.pdf
Author Kajiyama, Yuki| Mizobata, Satsuki| Akaji, Shusaku| Nemoto, Michiko| Tamura, Takashi| Inagaki, Kenji|
Abstract  Novel glycine oxidase (GlyOX) from Marinomonas mediterranea depends on cysteine tryptophilquinone (CTQ) and catalyzes the oxidative deamination of glycine to produce a glyoxylate, ammonia, and hydrogen peroxide. M. mediterranea GlyOX genes (goxA and goxB) were cloned and recombinant GlyOX was heterologously expressed by E. coli. The purification of recombinant GlyOX was carried out by metal affinity and DEAE-Toyopearl 650M column chromatographies. M. mediterranea GlyOX was homotetramic with a molecular mass of 76kDa and showed optimum activity around 30°C and at pH 5.0, and stability below 50°C and between pH 5.0 to 9.0. M. mediterranea GlyOX shows a strict substrate specificity toward glycine, and the Michaelis constant for glycine was 0.5mM. M. mediterranea GlyOX could determine the quantity of glycine in human serum and human blood plasma with high sensitivity. This study revealed the catalytic and structural properties of M. mediterranea GlyOX with high substrate specificity.
Keywords glycine oxidase Marinomonas mediterranea cysteine tryptophilquinone recombinant expression enzymatic glycine assay
Publication Title Scientific Reports of the Faculty of Agriculture, Okayama University
Published Date 2020-02-01
Volume volume109
Start Page 1
End Page 6
ISSN 2186-7755
language Japanese
File Version publisher
FullText URL fulltext.pdf
Author Ozuru, Ryo| Wakao, Shohei| Tsuji, Takahiro| Ohara, Naoya| Matsuba, Takashi| Amuran, Muhammad Y.| Isobe, Junko| Iino, Morio| Nishida, Naoki| Matsumoto, Sari| Iwadate, Kimiharu| Konishi, Noriko| Yasuda, Kaori| Tashiro, Kosuke| Hida, Misato| Yadoiwa, Arisato| Kato, Shinsuke| Yamashita, Eijiro| Matsumoto, Sohkichi| Kurozawa, Yoichi| Dezawa, Mari| Fujii, Jun|
Keywords Muse cells Shiga toxin-producing Escherichia coli acute encephalopathy
Published Date 2020-01-08
Publication Title Molecular Therapy
Volume volume28
Issue issue1
Publisher Elsevier
Start Page 100
End Page 118
ISSN 15250016
NCID AA11475060
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © 2019 The Author(s).
File Version publisher
PubMed ID 31607541
DOI 10.1016/j.ymthe.2019.09.023
Web of Science KeyUT 000506204400011
Related Url isVersionOf https://doi.org/10.1016/j.ymthe.2019.09.023
FullText URL fulltext.pdf Graphical_Abstract.pptx Figure1-6.pdf Supp_Info.pdf
Author Kanaya, Nobuhiko| Kuroda, Shinji| Kakiuchi, Yoshihiko| Kumon, Kento| Tsumura, Tomoko| Hashimoto, Masashi| Morihiro, Toshiaki| Kubota, Tetsushi| Aoyama, Katsuyuki| Kikuchi, Satoru| Nishizaki, Masahiko| Kagawa, Shunsuke| Tazawa, Hiroshi| Mizuguchi, Hiroyuki| Urata, Yasuo| Fujiwara, Toshiyoshi|
Keywords immune checkpoint programmed death-1 oncolytic adenovirus combined immunotherapy immunogenic cell death tumor infiltrating lymphocytes CD8 abscopal effect
Published Date 2020-01-10
Publication Title Molecular Therapy
Volume volume28
Issue issue3
Publisher Cell Press
Start Page 794
End Page 804
ISSN 15250016
NCID AA11475060
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
File Version author
PubMed ID 31991110
DOI 10.1016/j.ymthe.2020.01.003
Web of Science KeyUT 000518887200012
Related Url isVersionOf https://doi.org/10.1016/j.ymthe.2020.01.003
FullText URL fulltext.pdf
Author Oka, Aiko| Ninomiya, Takahiro| Fujiwara, Tazuko| Takao, Soshi| Sato, Yasuharu| Gion, Yuka| Minoura, Akira| Haruna, Shin-ichi| Yoshida, Naohiro| Sakuma, Yasunori| Izuhara, Kenji| Ono, Junya| Taniguchi, Masami| Haruna, Takenori| Higaki, Takaya| Kariya, Shin| Koyama, Takahisa| Takabayashi, Tetsuji| Imoto, Yoshimasa| Sakashita, Masafumi| Kidoguchi, Masanori| Nishizaki, Kazunori| Fujieda, Shigeharu| Okano, Mitsuhiro|
Keywords Chronic rhinosinusitis Eosinophils Eosinophils IgG4 Severity Surgery
Published Date 2020-01-14
Publication Title Allergology International
Publisher Elsevier
ISSN 13238930
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © 2019 Japanese Society of Allergology. Production and hosting by Elsevier B.V.
File Version publisher
PubMed ID 31952913
DOI 10.1016/j.alit.2019.12.004
Related Url isVersionOf https://doi.org/10.1016/j.alit.2019.12.004
FullText URL fulltext.pdf
Author Ueki, Yushi| Saito, Ken| Iioka, Hidekazu| Sakamoto, Izumi| Kanda, Yasuhiro| Sakaguchi, Masakiyo| Horii, Arata| Kondo, Eisaku|
Keywords Cancer Molecular Biology
Published Date 2020-01-18
Publication Title iScience
Volume volume23
Issue issue2
Publisher Cell Press
Start Page 100850
ISSN 25890042
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © 2020 The Author(s).
File Version publisher
PubMed ID 32058962
DOI 10.1016/j.isci.2020.100850
Web of Science KeyUT 000518637100047
Related Url isVersionOf https://doi.org/10.1016/j.isci.2020.100850
FullText URL fulltext.pdf
Author Isooka, Nami| Miyazaki, Ikuko| Kikuoka, Ryo| Wada, Kouichi| Nakayama, Erika| Shin, Kotaro| Yamamoto, Daichi| Kitamura, Yoshihisa| Asanuma, Masato|
Keywords Astrocyte Dopamine agonist Metallothionein Parkinson's disease Rotigotine Serotonin 1A receptor
Published Date 2020-01-31
Publication Title Neurochemistry International
Volume volume132
Publisher Elsevier
Start Page 104608
ISSN 01970186
NCID AA0032399X
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © 2019 The Authors. Published by Elsevier Ltd.
File Version publisher
PubMed ID 31765686
DOI 10.1016/j.neuint.2019.104608
Web of Science KeyUT 000508747200014
Related Url isVersionOf https://doi.org/10.1016/j.neuint.2019.104608
FullText URL fulltext.pdf
Author Suga, Michihiro| Shimada, Atsuhiro| Akita, Fusamichi| Shen, Jian-Ren| Tosha, Takehiko| Sugimoto, Hiroshi|
Keywords Heme Metalloproteins Proton pump Radiation damage Serial femtosecond crystallography X-ray free-electron laser
Published Date 2020-02-29
Publication Title Biochimica et Biophysica Acta (BBA) - General Subjects
Volume volume1864
Issue issue2
Publisher SI
Start Page 129466
ISSN 03044165
NCID AA00564679
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © 2019 The Author(s). Published by Elsevier B.V.
File Version publisher
PubMed ID 31678142
DOI 10.1016/j.bbagen.2019.129466
Web of Science KeyUT 000508740100002
Related Url isVersionOf https://doi.org/10.1016/j.bbagen.2019.129466
FullText URL fulltext.pdf
Author Watanabe, Atsuyuki| Fujimura, Osamu|
Keywords Tachycardiav Atrioventricular node reentry Cardiac defibrillatorIschemic cardiomyopathy
Published Date 2020-02-29
Publication Title Indian Pacing and Electrophysiology Journal
Volume volume20
Issue issue1
Publisher Elsevier
Start Page 35
End Page 38
ISSN 09726292
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © 2019 Indian Heart Rhythm Society. Production and hosting by Elsevier B.V.
File Version publisher
DOI 10.1016/j.ipej.2019.12.004
Related Url isVersionOf https://doi.org/10.1016/j.ipej.2019.12.004
FullText URL fulltext.pdf
Author Saito, Taichi| Noda, Tomoyuki| Kondo, Hiroya| Demiya, Koji| Nezu, Satoshi| Yokoo, Suguru| Matsuhashi, Minami| Uehara, Takenori| Shimamura, Yasunori| Kodama, Masayuki| Ozaki, Toshifumi|
Keywords Hand Induced membrane Masquelet technique Septic arthritis The small joint
Published Date 2020-02-29
Publication Title Trauma Case Reports
Volume volume25
Start Page 100268
ISSN 23526440
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © 2019 The Authors. Published by Elsevier Ltd.
File Version publisher
PubMed ID 1890833
DOI 10.1016/j.tcr.2019.100268
Related Url isVersionOf https://doi.org/10.1016/j.tcr.2019.100268
FullText URL BBRC520_3_600.pdf
Author Wei, Heng| Wang, Chen| Guo, Rui| Takahashi, Ken| Naruse, Keiji|
Keywords Cardiomyocytes Human induced pluripotent stem cells Ischemic heart disease Myocardial infarction
Published Date 2019-12-10
Publication Title Biochemical and Biophysical Research Communications
Volume volume520
Issue issue3
Publisher Academic Press
Start Page 600
End Page 605
ISSN 0006291X
NCID AA00564395
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © 2019 The Authors.
File Version publisher
PubMed ID 31623826
DOI 10.1016/j.bbrc.2019.09.119
Web of Science KeyUT 000506411300016
Related Url isVersionOf https://doi.org/10.1016/j.bbrc.2019.09.119
FullText URL fulltext.pdf
Author Ahmed, Fatma B.M.| Esmail, Mohamed F.C.| Kawahara, Nobuyuki| Tomita, Eiji|
Keywords Infrared absorption method CO2 HITRAN EGR Visualization Spark plug sensor
Published Date 2019-12-26
Publication Title Ain Shams Engineering Journal
Publisher Elsevier
ISSN 20904479
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © 2019 Ain Shams University. Production and hosting by Elsevier B.V.
File Version publisher
DOI 10.1016/j.asej.2019.12.003
Related Url isVersionOf https://doi.org/10.1016/j.asej.2019.12.003
FullText URL fulltext.pdf
Author Eto, Eriko| Maki, Jota| Akagi, Teiji| Ito, Hiroshi| Masuyama, Hisashi|
Keywords Anticoagulant Fontan circulation Labor analgesia Obstetrical complications Pregnancy
Published Date 2019-12-30
Publication Title Journal of Cardiology Cases
Volume volume21
Issue issue4
Publisher Elsevier
Start Page 161
End Page 163
ISSN 18785409
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © 2019 Published by Elsevier Ltd on behalf of Japanese College of Cardiology.
File Version publisher
PubMed ID 32256867
DOI 10.1016/j.jccase.2019.12.005
Related Url isVersionOf https://doi.org/10.1016/j.jccase.2019.12.005