Spontaneous neoplastic transformation in vitro of mouse whole embryo cells and newborn hamster lung cells

Three cell lines, spontaneously transformed in vitro, MWE-l and MWE-2 originating from C3Hf/Bi mouse whole embryos and NHLu-l originating from newborn syrian hamster lungs were obtained. These transformed cell lines were characterized by short generation time, marked aneuploidy in chromosome numbers and tumor induction in vivo. Histological appearance of the tumors induced by MWE-l was fibrosarcomatous. Myogenic character in MWE-2 and epithelial character in NHLu-l were interesting features.