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ID 17943
Eprint ID
17943
FullText URL
82_283.pdf 764 KB
Title Alternative
Studies on Glucose-6-Phosphatase and Glucokinase Activities in the Carcinogenesis of DAB fed Rat Hepatoma and Clinical Application of Serum Glucose-6-Phosphatase Activity
Author
Ishikawa, Makoto
Abstract
The carbohydrate metabolism in the carcinogenesis of DAB fed rat hepatoma was investigated by studying the G-6-Pase and Glucokinase activities to analyse the marked increase of glycolysis and decrease of respiration in the cancer tissue. And same time, these enzymes activities were measured in embrionic, new born, and regeneratig rat liver, which showed rapid growth and cell division, to study whether there was biochemical specificity in hepatoma. The liver homogenate G-6-Pase activity decreased progressively along with DAB feeding and was almost absent in the hepatoma tissue, while the glucokinase activity increased gradually and about 4 times of normal activity in cancer tissue. On the other hand, there was no change of glucokinase activity in the embrionic, new born, regenerating, or normal rat liver. And the G-6-Pase activity was almost absent in the embrionic rat liver and showed rapid increase after birth to maximum in 12th day born rat liver. From these results, both enzymes have important significance in the elevated glycolysis in cancer, although only G-6-Pase seems rate limiting factor in the physiologically elevated glycolysis state of the liver. So difinite difference was recognized between hepatoma and these tissues biochemically. The serum G-6-Pase activities in the carcinogenesis of DAB fed rat showed no significant change from normal rat. For clinical application, the serum G-6-Pase activities of various patients were also measured. Patients with hepatic disturbance especially acute hepatitis or active hepatitis demonstrated significant elevation of the G-6-Pase activity, while no other patient did not. So the measurement of serum G-6-Pase activity is very useful in clinical aspect.
Published Date
1970-06-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume82
Issue
issue5-6
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
283
End Page
294
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/82/5-6/82_5-6_283/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
Japanese
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma