肺胞マクロファージのスーパーオキサイド産生能に関する研究 第2編 高血糖モルモットの肺胞マクロファージのスーパーオキサイド産生能と過酸化脂質生成能に関する研究

Patients with diabetes mellitus are susceptible to infections, due to the disturbance of host defense mechanisms, such as chemotaxis, phagocytosis and killing of bacteria. In this experiment superoxide production and lipid peroxide production, by peroxidation of unsaturated fatty acid, were examined. The superoxide production activity of PAM in hyperglycemics was significantly lower(P<0.005) than that in normal controls. The values were 5.68±1.68 nmol/10(6) cells/min in the former and 8.20±2.60 nmol/10(6) cells/min in the latter. The lipid peroxide production activity (△TBARS) in hyperglycemics (0.06±0.04 nmol/10(6) cells) was lower(P<0.05) than that of normal controls (0.10±0.07 nmol/10(6) cells). The lipid peroxide production activity (△TBARS) was proportionate to the superoxide production activity(r=0.570, P<0.02). Therefore, the △TBARS may depend on superoxide production. There was no significant difference in superoxide dismutase(SOD) activity between hyperglycemics and normal controls. The SOD activity was proportionate to the superoxide production activity in normal controls, and thus, SOD activity seems to depend on superoxide production.