ID | 56198 |
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Author |
Takeda, Tetsuya
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Kozai, Toshiya
Department of Physics, College of Science and Engineering, Kanazawa University
Yang, Huiran
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Ishikuro, Daiki
Department of Physics, College of Science and Engineering, Kanazawa University
Seyama, Kaho
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Kumagai, Yusuke
Department of Physics, College of Science and Engineering, Kanazawa University
Abe, Tadashi
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Yamada, Hiroshi
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Uchihashi, Takayuki
CREST, JST
Ando, Toshio
CREST, JST
Takei, Kohji
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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Abstract | Dynamin is a mechanochemical GTPase essential for membrane fission during clathrin-mediated endocytosis. Dynamin forms helical complexes at the neck of clathrin-coated pits and their structural changes coupled with GTP hydrolysis drive membrane fission. Dynamin and its binding protein amphiphysin cooperatively regulate membrane remodeling during the fission, but its precise mechanism remains elusive. In this study, we analyzed structural changes of dynamin-amphiphysin complexes during the membrane fission using electron microscopy (EM) and high-speed atomic force microscopy (HS-AFM). Interestingly, HS-AFM analyses show that the dynamin-amphiphysin helices are rearranged to form clusters upon GTP hydrolysis and membrane constriction occurs at protein-uncoated regions flanking the clusters. We also show a novel function of amphiphysin in size control of the clusters to enhance biogenesis of endocytic vesicles. Our approaches using combination of EM and HS-AFM clearly demonstrate new mechanistic insights into the dynamics of dynamin-amphiphysin complexes during membrane fission.
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Keywords | EM
HS-AFM
amphiphysin
biophysics
cell biology
dynamin
human
in vitro reconstitution
membrane remodeling
structural biology
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Published Date | 2018-01
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Publication Title |
eLife
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Volume | volume7
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Publisher | eLife Sciences Publications
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Start Page | e30246
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ISSN | 2050-084X
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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File Version | publisher
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PubMed ID | |
DOI | |
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Related Url | isVersionOf https://doi.org/10.7554/eLife.30246
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