ID | 58082 |
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Author |
Wang, Pingyu
Shanghai Center for Plant Stress Biology, Center of Excellence for Molecular Plant Sciences, Chinese Academy of Sciences
Yao, Shaolun
Shanghai Center for Plant Stress Biology, Center of Excellence for Molecular Plant Sciences, Chinese Academy of Sciences
Kosami, Ken-ichi
Shanghai Center for Plant Stress Biology, Center of Excellence for Molecular Plant Sciences, Chinese Academy of Sciences
Guo, Ting
Shanghai Center for Plant Stress Biology, Center of Excellence for Molecular Plant Sciences, Chinese Academy of Sciences
Li, Jing
Shanghai Center for Plant Stress Biology, Center of Excellence for Molecular Plant Sciences, Chinese Academy of Sciences
Zhang, Yuanyuan
Shanghai Center for Plant Stress Biology, Center of Excellence for Molecular Plant Sciences, Chinese Academy of Sciences
Fukao, Yoichiro
Department of Bioinformatics, Ritsumeikan University
Kaneko-Kawano, Takako
College of Pharmaceutical Sciences, Ritsumeikan University
Zhang, Heng
Shanghai Center for Plant Stress Biology, Center of Excellence for Molecular Plant Sciences, Chinese Academy of Sciences
She, Yi-Min
Shanghai Center for Plant Stress Biology, Center of Excellence for Molecular Plant Sciences, Chinese Academy of Sciences
Wang, Pengcheng
Shanghai Center for Plant Stress Biology, Center of Excellence for Molecular Plant Sciences, Chinese Academy of Sciences
Xing, Weiman
Biomolecular Structure and Design, Shanghai Center for Plant Stress Biology
Hanada, Kousuke
Department of Bioscience and Bioinformatics, Kyushu Institute of Technology
Liu, Renyi
Center for Agroforestry Mega Data Science and FAFU-UCR Joint Center for Horticultural Biology and Metabolomics, Haixia Institute of Science and Technology,Fujian Agriculture and Forestry University
Kawano, Yoji
Institute of Plant Science and Resources, Okayama University
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Abstract | Small signalling peptides, generated from larger protein precursors, are important components to orchestrate various plant processes such as development and immune responses. However, small signalling peptides involved in plant immunity remain largely unknown. Here, we developed a pipeline using transcriptomics- and proteomics-based screening to identify putative precursors of small signalling peptides: small secreted proteins (SSPs) in rice, induced by rice blast fungus Magnaporthe oryzae and its elicitor, chitin. We identified 236 SSPs including members of two known small signalling peptide families, namely rapid alkalinization factors and phytosulfokines, as well as many other protein families that are known to be involved in immunity, such as proteinase inhibitors and pathogenesis-related protein families. We also isolated 52 unannotated SSPs and among them, we found one gene which we named immune response peptide (IRP) that appeared to encode the precursor of a small signalling peptide regulating rice immunity. In rice suspension cells, the expression of IRP was induced by bacterial peptidoglycan and fungal chitin. Overexpression of IRP enhanced the expression of a defence gene, PAL1 and induced the activation of the MAPKs in rice suspension cells. Moreover, the IRP protein level increased in suspension cell medium after chitin treatment. Collectively, we established a simple and efficient pipeline to discover SSP candidates that probably play important roles in rice immunity and identified 52 unannotated SSPs that may be useful for further elucidation of rice immunity. Our method can be applied to identify SSPs that are involved not only in immunity but also in other plant functions.
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Keywords | immunity
Magnaporthe oryzae
proteomics
transcriptomics
rice
signalling peptide
small secreted protein
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Published Date | 2019-07-13
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Publication Title |
Plant Biotechnology Journal
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Volume | volume18
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Issue | issue2
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Publisher | Wiley
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Start Page | 415
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End Page | 428
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ISSN | 1467-7644
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NCID | AA11805223
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © 2019 The Authors.
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File Version | publisher
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DOI | |
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Related Url | isVersionOf https://doi.org/10.1111/pbi.13208
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License | https://creativecommons.org/licenses/by-nc/4.0/
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