ID | 60852 |
FullText URL | |
Author |
Maeda, Takanori
College of Bioresource Sciences, Nihon University
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Abstract | Orchid fleck virus (OFV) has a bipartite negative-sense RNA genome with sequence similarities to plant rhabdoviruses. The non-enveloped bullet-shaped particles of OFV are similar to those of the internal ribonucleoprotein (RNP)-M protein structure of rhabdoviruses, but they are about half the size of typical plant rhabdoviruses. Purified preparations contained intact bullet-shaped and filamentous particles. The filamentous particles showed a tightly coiled coil structure or a coiled structure with a helical twist, which resembles the RNP complex of rhabdoviruses. OFV bullet-shaped particles were structurally stable in solutions containing 2% Triton X-100 and 0.8 M NaCl. Western blot analyses revealed that the bullet-shaped particles contained N, P and M proteins, while filamentous particles contained mainly N and P proteins. In addition, a small amount of the L protein was detected in both types of particles. Thus, the structural proteins of OFV have properties similar to those of rhabdoviruses, except that the particles are non-enveloped and are relatively resistant to detergent-treatment under high-salt conditions.
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Keywords | Rabies
ORF4 Protein
Sucrose Density Gradient Centrifugation
Coil Coil Structure
Potential Glycosylation Site
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Note | This is a post-peer-review, pre-copyedit version of an article published in Archives of Virology. The final authenticated version is available online at: http://dx.doi.org/10.1007/s00705-008-0268-6.
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Published Date | 2008-12-06
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Publication Title |
Archives of Virology
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Volume | volume154
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Issue | issue1
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Publisher | Springer
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Start Page | 37
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End Page | 45
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ISSN | 0304-8608
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NCID | AA00548901
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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File Version | author
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PubMed ID | |
DOI | |
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Related Url | isVersionOf https://doi.org/10.1007/s00705-008-0268-6
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