ID | 52782 |
FullText URL | |
Author |
Hayashi, Tatsuro
Asano, Hiroaki
Kaken ID
Toyooka, Shinichi
Maki, Yuho
Tanaka, Norimitsu
Doihara, Hiroyoshi
Kaken ID
researchmap
Huh, Nam-ho
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Abstract | The REIC (reduced expression in immortalized cells)/Dkk-3 is down-regulated in various cancers and considered to be a tumor suppressor gene. REIC/Dkk-3 mRNA has two isoforms (type-a,b). REIC type-a mRNA has shown to be a major transcript in various cancer cells, and its promoter activity was much stronger than that of type-b. In this study, we examined the methylation status of REIC/Dkk-3 type-a in a broad range of human malignancies.
We examined REIC/Dkk-3 type-a methylation in breast cancers, non-small-cell lung cancers, gastric cancers, colorectal cancers, and malignant pleural mesotheliomas using a quantitative combined bisulfite restriction analysis assay and bisulfate sequencing. REIC/Dkk-3 type-a and type-b expression was examined using reverse transcriptional PCR. The relationships between the methylation and clinicopathological factors were analyzed.
The rate of REIC/Dkk-3 type-a methylation ranged from 26.2 to 50.0% in the various primary tumors that were examined. REIC/Dkk-3 type-a methylation in breast cancer cells was significantly heavier than that in the other cell lines that we tested. REIC/Dkk-3 type-a methylation was inversely correlated with REIC/Dkk-3 type-a expression. There was a correlation between REIC/Dkk-3 type-a and type-b mRNA expression. REIC/Dkk-3 type-a expression was restored in MDA-MB-231 cells using 5-aza-2'-deoxycytidine treatment. We found that estrogen receptor-positive breast cancers were significantly more common among the methylated group than among the non-methylated group.
REIC/Dkk-3 type-a methylation was frequently detected in a broad range of cancers and appeared to play a key role in silencing REIC/Dkk-3 type-a expression in these malignancies.
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Keywords | DNA methylation
REIC/Dkk-3
Breast cancer
Lung cancer
Mesothelioma
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Published Date | 2012-05
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Publication Title |
Journal of Cancer Research and Clinical Oncology
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Volume | volume138
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Issue | issue5
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Publisher | Springer
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Start Page | 799
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End Page | 809
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ISSN | 0171-5216
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Content Type |
Journal Article
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Related Url | http://ousar.lib.okayama-u.ac.jp/metadata/52511
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language |
English
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File Version | author
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Refereed |
True
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DOI | |
Web of Science KeyUT |