ID | 50622 |
FullText URL | |
Author |
Jiyou, Wang
Katoh, Keizo
Senoh, Mitsutoshi
Mizuno, Tamaki
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Maehara, Yoko
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Abstract | Vibrio vulnificus is a ubiquitous estuarine microorganism but causes fatal systemic infections in immunocompromised humans, cultured eels or shrimps. An extracellular metalloprotease VVP/VvpE has been reported to be a potential virulence factor of the bacterium; however, a few strains isolated from a diseased eel or shrimp were recently found to produce a serine protease termed VvsA, but not VVP/VvpE. In the present study, we found that these strains had lost the 80 kb genomic region including the gene encoding VVP/VvpE. We also purified VvsA from the culture supernatant through ammonium sulfate fractionation, gel filtration and ion-exchange column chromatography, and the enzyme was demonstrated to be a chymotrypsin-like protease, as well as those from some vibrios. The gene vvsA was shown to constitute an operon with a downstream gene vvsB, and several Vibrio species were found to have orthologues of vvsAB. These findings indicate that the genes vvp/vvpE and vvsAB might be mobile genetic elements.
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Keywords | Polymerase chain reaction
Purification
Serine protease
Metalloprotease
Vibrio vulnificus
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Published Date | 2012-04
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Publication Title |
World Journal of Microbiology & Biotechnology
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Volume | volume28
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Issue | issue4
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Publisher | Springer
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Start Page | 1633
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End Page | 1639
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ISSN | 0959-3993
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Content Type |
Journal Article
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Project |
Collaborative Research of Okayama University for Infectious Diseases in India
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Official Url | http://dx.doi.org/10.1007/s11274-011-0969-y
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language |
English
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File Version | author
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Refereed |
True
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DOI | |
Web of Science KeyUT |