ID | 65239 |
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Author |
Shirakawa, Riko
Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
Ishikawa, Kazuya
Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
Furuta, Kazuyuki
Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
Kaito, Chikara
Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
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Abstract | Zinc is an essential metal for cells, but excess amounts are toxic. Other than by regulating the intracellular zinc concentration by zinc uptake or efflux, the mechanisms underlying bacterial resistance to excess zinc are unknown. In the present study, we searched for zinc-resistant mutant strains from the Keio collection, a gene knockout library of Escherichia coli, a model gram-negative bacteria. We found that knockout mutant of RpmJ (L36), a 50S ribosomal protein, exhibited zinc resistance. The rpmJ mutant was sensitive to protein synthesis inhibitors and had altered translation fidelity, indicating ribosomal dysfunction. In the rpmJ mutant, the intracellular zinc concentration was decreased under excess zinc conditions. Knockout of ZntA, a zinc efflux pump, abolished the zinc-resistant phenotype of the rpmJ mutant. RNA sequence analysis revealed that the rpmJ mutant exhibited altered gene expression of diverse functional categories, including translation, energy metabolism, and stress response. These findings suggest that knocking out RpmJ alters gene expression patterns and causes zinc resistance by lowering the intracellular zinc concentration. Knockouts of other ribosomal proteins, including RplA, RpmE, RpmI, and RpsT, also led to a zinc-resistant phenotype, suggesting that deletion of ribosomal proteins is closely related to zinc resistance.
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Published Date | 2023-03-24
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Publication Title |
PLoS ONE
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Volume | volume18
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Issue | issue3
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Publisher | Public Library of Science
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ISSN | 1932-6203
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © 2023 Shirakawa et al.
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File Version | publisher
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Related Url | isVersionOf https://doi.org/10.1371/journal.pone.0277162
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License | https://creativecommons.org/licenses/by/4.0/
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Citation | Shirakawa R, Ishikawa K, Furuta K, Kaito C (2023) Knockout of ribosomal protein RpmJ leads to zinc resistance in Escherichia coli. PLoS ONE 18(3): e0277162. https://doi.org/10.1371/journal.pone.0277162
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Funder Name |
Japan Society for the Promotion of Science
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助成番号 | 22K14892
22H02869
22K19435
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