ID | 65034 |
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Author |
Hatanaka, Kazu
Department of Periodontics and Endodontics, Okayama University Hospital
Shirahase, Yasushi
Sysmex Corporation
Yoshida, Toshiyuki
Sysmex Corporation
Kono, Mari
Sysmex Corporation
Toya, Naoki
Sysmex Corporation
Sakasegawa, Shin-Ichi
Asahi Kasei Pharma Corporation
Konishi, Kenji
Asahi Kasei Pharma Corporation
Yamamoto, Tadashi
Department of Pathophysiology-Periodontal Science, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine
Ochiai, Kuniyasu
Department of Pathophysiology-Periodontal Science, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine
Takashiba, Shogo
Department of Pathophysiology-Periodontal Science, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine
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Abstract | Periodontal disease is a chronic inflammatory condition caused by periodontal pathogens in the gingival sulcus. Short-chain fatty acids (SCFAs) produced by causal bacteria are closely related to the onset and progression of periodontal disease and have been reported to proliferate in the periodontal sulcus of patients experiencing this pathology. In such patients, propionic acid (C3), butyric acid (C4), isobutyric acid (IC4), valeric acid (C5), isovaleric acid (IC5), and caproic acid (C6), henceforth referred to as [C3-C6], has been reported to have a detrimental effect, while acetic acid (C2) exhibits no detrimental effect. In this study, we established an inexpensive and simple enzymatic assay that can fractionate and measure these acids. The possibility of applying this technique to determine the severity of periodontal disease by adapting it to specimens collected from humans has been explored. We established an enzyme system using acetate kinase and butyrate kinase capable of measuring SCFAs in two fractions, C2 and [C3-C6]. The gingival crevicular fluid (GCF) and saliva of 10 healthy participants and 10 participants with mild and severe periodontal disease were measured using the established enzymatic method and conventional gas chromatography-mass spectrometry (GC-MS). The quantification of C2 and [C3-C6] in human GCF and saliva was well correlated when using the GC-MS method. Furthermore, both C2 and [C3-C6] in the GCF increased with disease severity. However, while no significant difference was observed between healthy participants and periodontal patients when using saliva, [C3-C6] significantly differed between mild and severe periodontal disease. The enzymatic method was able to measure C2 and [C3-C6] separately as well as using the GC-MS method. Furthermore, the C2 and [C3-C6] fractions of GCF correlated with disease severity, suggesting that this method can be applied clinically. In contrast, the quantification of C2 and [C3-C6] in saliva did not differ significantly between healthy participants and patients with periodontal disease. Future studies should focus on inflammation rather than on tissue destruction.
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Published Date | 2022-07-15
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Publication Title |
PLoS ONE
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Volume | volume17
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Issue | issue7
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Publisher | Public Library of Science
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ISSN | 1932-6203
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © 2022 Hatanaka et al.
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File Version | publisher
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DOI | |
Web of Science KeyUT | |
Related Url | isVersionOf https://doi.org/10.1371/journal.pone.0268671
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License | https://creativecommons.org/licenses/by/4.0/
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Citation | Hatanaka K, Shirahase Y, Yoshida T, Kono M, Toya N, Sakasegawa S-i, et al. (2022) Enzymatic measurement of short-chain fatty acids and application in periodontal disease diagnosis. PLoS ONE 17(7): e0268671. https://doi.org/10.1371/ journal.pone.0268671
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Funder Name |
Sysmex Corporation
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