ID | 31002 |
JaLCDOI | |
FullText URL | |
Author |
Miyazaki, Masahiro
Suzuki, Yasunori
Sato, Jiro
|
Abstract | A method has been developed for the rapid preparation of single-cell suspensions from rat hepatocyte primary cultures on collagen substratum. Hepatocytes were adequately dissociated into single cells when the cultures were first treated with a combination of trypsin and ethylenediaminetetraacetic acid (EDTA) and then with collagenase. However, when the order was reversed, hepatocytes were inadequately dispersed. The possible mechanism of cell dissociation is discussed on the basis of the experimental data obtained. |
Keywords | primary culture
rat hepatocytes
collagen substratum
cell dissociation
|
Amo Type | Article
|
Publication Title |
Acta Medica Okayama
|
Published Date | 1988-12
|
Volume | volume42
|
Issue | issue6
|
Publisher | Okayama University Medical School
|
Start Page | 351
|
End Page | 354
|
ISSN | 0386-300X
|
NCID | AA00508441
|
Content Type |
Journal Article
|
language |
English
|
File Version | publisher
|
Refereed |
True
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PubMed ID | |
Web of Science KeyUT |