Partial purification of Simian virus 40 large T antigen by immunoaffinity chromatography and its detection by enzyme-linked immunosorbent assay.

Ikeda, Shogo; Hatsushika, Masao; Shigehara, Tsuguya; Watanabe, Sekiko; Omura, Sachiko; Tsutsui, Ken; Oda, Takuzo

ID	30311
JaLCDOI	10.18926/AMO/30311
FullText URL	fulltext.pdf 652 KB
Author	Ikeda, Shogo Hatsushika, Masao Shigehara, Tsuguya Watanabe, Sekiko Omura, Sachiko Tsutsui, Ken Kaken ID publons researchmap Oda, Takuzo
Abstract	Simian virus 40 (SV40) large T antigen was partially purified from small amounts of SV40-infected and SV40-transformed cells by immunoaffinity chromatography with high recovery. T antigen, in both crude and partially purified states, was detected rapidly by a sensitive and quantitative enzyme-linked immunosorbent assay (ELISA). Stability of the partially purified T antigen was found to increase by addition of 0.01% bovine serum albumin (BSA).
Keywords	SV40 T antigen affinity chromatography ELISA
Amo Type	Article
Publication Title	Acta Medica Okayama
Published Date	1984-08
Volume	volume38
Issue	issue4
Publisher	Okayama University Medical School
Start Page	341
End Page	347
ISSN	0386-300X
NCID	AA00508441
Content Type	Journal Article
language	English
File Version	publisher
Refereed	True
PubMed ID	6093443
Web of Science KeyUT	A1984TG25900003