ID | 30710 |
JaLCDOI | |
FullText URL | |
Author |
Ogata, Masana
Mizugaki, Junko
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Abstract | Erythrocyte catalase in normal and Japanese type acatalasemia hemolysates was separated by chromatofocusing into several fractions in the pH range of 6.1 to 5.7. Normal hemolysate gave a major peak of catalase activity with a pH of 6.1 to 5.5, while acatalasemia hemolysate gave several small peaks in this pH range and a main peak with a pH of 6.6 to 6.2. The main protein band in catalase active fractions separated from normal erythrocytes had a molecular weight of 60,000 by SDS polyacrylamide gel electrophoresis. A similar faint protein band having a molecular weight of 60,000 was also found in acatalasemia hemolysate in addition to a fairly intense band with a molecular weight of about 30,000. Catalase active fractions from normal erythrocytes reacted with antihuman erythrocyte catalase rabbit serum by double immunodiffusion. |
Keywords | acatalasemia
SDS polyacrylamide gel electrophoresis
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Amo Type | Article
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Publication Title |
Acta Medica Okayama
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Published Date | 1982-02
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Volume | volume36
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Issue | issue1
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Publisher | Okayama University Medical School
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Start Page | 73
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End Page | 76
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ISSN | 0386-300X
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NCID | AA00508441
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Content Type |
Journal Article
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language |
English
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File Version | publisher
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Refereed |
True
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PubMed ID | |
Web of Science KeyUT |