ID | 31022 |
JaLCDOI | |
FullText URL | |
Author |
Masuoka, Noriyoshi
Ubuka, Toshihiko
Kinuta, Masahiro
Yoshida, Shigeko
Taguchi, Tazuko
|
Abstract | A new gas chromatographic method for the determination of sulfate was developed. In this method, sulfate was quantitatively converted to a volatile derivative, dimethyl sulfate, by a two-step procedure. First, sulfate was converted to silver sulfate by reaction with silver oxide, and then to dimethyl sulfate by reaction with methyl iodide. The derivative was analyzed by gas chromatography. Methyl methanesulfonate was used as an internal standard. The method was applied to the determination of total urinary sulfate. Phosphate and chloride ions, which interfered with the present method, were eliminated with the use of basic magnesium carbonate and an excess of silver oxide, respectively. Recovery was over 96% when 5 to 40 mumol/ml of sulfate was added to human urine samples. |
Keywords | gas chromatography
sulfate determination
dimethy1 sulfate
sulfuric acid
urinary sulfate
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Amo Type | Article
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Publication Title |
Acta Medica Okayama
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Published Date | 1988-10
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Volume | volume42
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Issue | issue5
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Publisher | Okayama University Medical School
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Start Page | 247
|
End Page | 252
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ISSN | 0386-300X
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NCID | AA00508441
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Content Type |
Journal Article
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language |
English
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File Version | publisher
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Refereed |
True
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PubMed ID | |
Web of Science KeyUT |