ID | 57788 |
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Author |
Toba, Shinsuke
Department of Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Minato, Yusuke
Department of Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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Kondo, Yuma
Department of Microbiology, Institute of Biomedical & Health Sciences, Hiroshima University
Hoshikawa, Kanami
Department of Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Minagawa, Shu
Komaki, Shiho
Department of Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Kumagai, Takanori
Department of Microbiology, Institute of Biomedical & Health Sciences, Hiroshima University
Matoba, Yasuyuki
Department of Microbiology, Institute of Biomedical & Health Sciences, Hiroshima University
Morita, Daichi
Department of Microbiology, Institute of Biomedical & Health Sciences, Hiroshima University
Ogawa, Wakano
Department of Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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Gotoh, Naomasa
Department of Microbiology and Infection Control Science, Kyoto Pharmaceutical University
Tsuchiya, Tomofusa
Department of Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Kuroda, Teruo
Department of Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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Abstract | We investigated the role of the resistance-nodulation-cell division superfamily (RND) efflux system on intrinsic multidrug resistance in Serratia marcescens. We identified eight putative RND efflux system genes in the S. marcescens Db10 genome that included the previously characterized systems, sdeXY, sdeAB, and sdeCDE. Six out of the eight genes conferred multidrug resistance on KAM32, a drug hypersensitive strain of Escherichia coil. Five out of the eight genes conferred resistance to benzalkonium, suggesting the importance of RND efflux systems in biocide resistance in S. marcescens. The energy-dependent efflux activities of five of the pumps were examined using a rhodamine 6G efflux assay. When expressed in the toiC-deficient strain of E. coil, KAM43, none of the genes conferred resistance on E. coil. When hasF, encoding the S. marcescens ToIC ortholog, was expressed in KAM43, all of the genes conferred resistance on E. coil, suggesting that HasF is a major outer membrane protein that is used by all RND efflux systems in this organism. We constructed a sdeXY deletion mutant from a derivative strain of the clinically isolated multidrug-resistant S. marcescens strain and found that the sdeXY deletion mutant was sensitive to a broad spectrum of antimicrobial agents.
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Published Date | 2019-3-19
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Publication Title |
Scientific Reports
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Volume | volume9
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Publisher | Nature Publishing Group
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Start Page | 4854
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ISSN | 2045-2322
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © The Author(s) 2019
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File Version | publisher
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PubMed ID | |
DOI | |
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Related Url | isVersionOf https://doi.org/10.1038/s41598-019-41237-7
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License | https://creativecommons.org/licenses/by/4.0/
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Funder Name |
Japan Society for the Promotion of Science
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助成番号 | JP24590080
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