ID | 66134 |
FullText URL | |
Author |
Kim, June-Sik
Institute of Plant Science and Resources, Okayama University
Takahashi, Hidekazu
Faculty of Food and Agricultural Sciences, Fukushima University
Yajima, Shunsuke
NODAI Genome Research Center, Tokyo University of Agriculture
Koshiishi, Yuichi
NODAI Genome Research Center, Tokyo University of Agriculture
Sotome, Toshinori
Tochigi Prefectural Agricultural Experiment Station
Kato, Tsuneo
Tochigi Prefectural Agricultural Experiment Station
Mochida, Keiichi
Bioproductivity Informatics Research Team, RIKEN Center for Sustainable Resource Science
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Abstract | Two modern high-quality Japanese malting barley cultivars, ‘Sukai Golden’ and ‘Sachiho Golden’, were subjected to RNA-sequencing of transcripts extracted from 20-day-old immature seeds. Despite their close relation, 2,419 Sukai Golden-specific and 3,058 Sachiho Golden-specific SNPs were detected in comparison to the genome sequences of two reference cultivars: ‘Morex’ and ‘Haruna Nijo’. Two single nucleotide polymorphism (SNP) clusters respectively showing the incorporation of (1) the barley yellow mosaic virus (BaYMV) resistance gene rym5 from six-row non-malting Chinese landrace Mokusekko 3 on the long arm of 3H, and (2) the anthocyanin-less ant2 gene from a two-row Dutch cultivar on the long arm of 2H were detected specifically in ‘Sukai Golden’. Using 221 recombinant inbred lines of a cross between ‘Ishukushirazu’ and ‘Nishinochikara’, another BaYMV resistance rym3 gene derived from six-row non-malting Japanese cultivar ‘Haganemugi’ was mapped to a 0.4-cM interval on the proximal region of 5H. Haplotype analysis of progenitor accessions of the two modern malting cultivars revealed that rym3 of ‘Haganemugi’ was independently introduced into ‘Sukai Golden’ and ‘Sachiho Golden’. Residual chromosome 5H segments of ‘Haganemugi’ surrounding rym3 were larger in ‘Sukai Golden’. Available results suggest possibilities for malting quality improvement by minimizing residual segments surrounding rym3.
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Keywords | genetic diversity
Hordeum vulgare
RNA-sequencing
seed transcriptome
single nucleotide polymorphism
virus disease resistance genes
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Published Date | 2023
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Publication Title |
Breeding Science
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Volume | volume73
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Issue | issue5
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Publisher | Japanese Society of Breeding
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Start Page | 435
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End Page | 444
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ISSN | 1344-7610
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NCID | AA11353132
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © 2023 Japanese Society of Breeding.
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File Version | publisher
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DOI | |
Web of Science KeyUT | |
Related Url | isVersionOf https://doi.org/10.1270/jsbbs.23031
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License | https://creativecommons.org/licenses/by/4.0/
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