ID | 47025 |
JaLCDOI | |
Sort Key | 6
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FullText URL | |
Author |
Hikida, Masaki
Nakayama, Yasunori
Kanayama, Naoki
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Abstract | A population of peripheral B cells have been shown to express recombination activating gene products, RAG-1 and RAG-2, which are considered to be involved in revising the B cell antigen receptor (BCR) in the periphery. BCR engagement has been reported to turn off RAG expression in peripheral B cells, whereas the same treatment has an opposite effect in immature B cells in the bone marrow. In contrast to receptor editing that is involved in the removal of autoreactivity in immature B cells, it has been shown that secondary V(D)J rearrangement in peripheral B cells, termed receptor revision, contributes to affinity maturation of antibodies. Here, we show that RAG-2 expression in murine splenic B cells was abrogated by the coligation of BCR with complement receptors (CD21/CD35) much more efficiently than by the engagement of BCR alone. On the other hand, the same coligation augmented proliferation of anti-CD40-stimulated B cells. Consistent with these observations, RAG-2 expression was lower in the draining lymph nodes of the quasi-monoclonal mice when they were immunized with a high-affinity antigen than with a low-affinity one. These findings suggest a crucial role for CD21/CD35 in directing the conservation or the revision of
BCRs in peripheral B cells.
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Publication Title |
Memoirs of the Faculty of Engineering, Okayama University
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Published Date | 2002-03
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Volume | volume36
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Issue | issue2
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Publisher | Faculty of Engineering, Okayama University
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Start Page | 51
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End Page | 60
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ISSN | 0475-0071
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NCID | AA10699856
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Content Type |
Departmental Bulletin Paper
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OAI-PMH Set |
岡山大学
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language |
English
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File Version | publisher
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NAID | |
Eprints Journal Name | mfe
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