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Kondo, Hideki Institute of Plant Science and Resources (IPSR), Okayama University Kaken ID publons researchmap
Hisano, Sakae Institute of Plant Science and Resources (IPSR), Okayama University
Chiba, Sotaro Institute of Plant Science and Resources (IPSR), Okayama University
Maruyama, Kazuyuki Institute of Plant Science and Resources (IPSR), Okayama University
Andika, Ida Bagus Institute of Plant Science and Resources (IPSR), Okayama University
Toyoda, Kazuhiro Graduate School of Environmental and Life Science, Okayama University
Fujimori, Fumihiro Department of Environmental Education, Tokyo Kasei University
Suzuki, Nobuhiro Institute of Plant Science and Resources (IPSR), Okayama University
Abstract
The identification of mycoviruses contributes greatly to understanding of the diversity and evolutionary aspects of viruses. Powdery mildew fungi are important and widely studied obligate phytopathogenic agents, but there has been no report on mycoviruses infecting these fungi. In this study, we used a deep sequencing approach to analyze the double-stranded RNA (dsRNA) segments isolated from field-collected samples of powdery mildew fungus-infected red clover plants in Japan. Database searches identified the presence of at least ten totivirus (genus Totivirus)-like sequences, termed red clover powdery mildew-associated totiviruses (RPaTVs). The majority of these sequences shared moderate amino acid sequence identity with each other (<44%) and with other known totiviruses (<59%). Nine of these identified sequences (RPaTV1a, 1b and 2-8) resembled the genome of the prototype totivirus, Saccharomyces cerevisiae virus-L-A (ScV-L-A) in that they contained two overlapping open reading frames (ORFs) encoding a putative coat protein (CP) and an RNA dependent RNA polymerase (RdRp), while one sequence (RPaTV9) showed similarity to another totivirus, Ustilago maydis virus H1 (UmV-H1) that encodes a single polyprotein (CP-RdRp fusion). Similar to yeast totiviruses, each ScV-L-A-like RPaTV contains a -1 ribosomal frameshift site downstream of a predicted pseudoknot structure in the overlapping region of these ORFs, suggesting that the RdRp is translated as a CP-RdRp fusion. Moreover, several ScV-L-A-like sequences were also found by searches of the transcriptome shotgun assembly (TSA) libraries from rust fungi, plants and insects. Phylogenetic analyses show that nine ScV-L-A-like RPaTVs along with ScV-L-A-like sequences derived from TSA libraries are clustered with most established members of the genus Totivirus, while one RPaTV forms a new distinct clade with UmV-H1, possibly establishing an additional genus in the family. Taken together, our results indicate the presence of diverse, novel totiviruses in the powdery mildew fungus populations infecting red clover plants in the field.
Keywords
Deep sequencing
Double stranded RNA virus
Powdery mildew
Saccharomyces cerevisiae virus L-A
Totivirus
Ustilago maydis virus H1
Published Date
2016-02
Publication Title
Virus Research
Volume
volume213
Publisher
Elsevier Science
Start Page
353
End Page
364
ISSN
01681702
NCID
AA10642076
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
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author
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DOI
Web of Science KeyUT
Related Url
isVersionOf https://doi.org/10.1016/j.virusres.2015.11.015
Citation
Hideki Kondo, Sakae Hisano, Sotaro Chiba, Kazuyuki Maruyama, Ida Bagus Andika, Kazuhiro Toyoda, Fumihiro Fujimori, Nobuhiro Suzuki, Sequence and phylogenetic analyses of novel totivirus-like double-stranded RNAs from field-collected powdery mildew fungi, Virus Research, Volume 213, 2016, Pages 353-364, ISSN 0168-1702, https://doi.org/10.1016/j.virusres.2015.11.015.
Funder Name
Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT) Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research (KAKENHI)
助成番号
15K07312 : メタゲノム解析に基づく菌類ウイルス叢の解明と有効利用に関する研究
25252011 : 植物病原糸状菌の抗ウイルス自然免疫機構