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Okubo, Keisuke Department of Pathophysiology - Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Ito, Takashi Center for Innovative Clinical Medicine, Okayama University Hospital
Shiota, Yasuyoshi Department of Pathophysiology - Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kawata, Yusuke Department of Pathophysiology - Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Yamamoto, Tadashi Department of Pathophysiology - Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Takashiba, Shogo Department of Pathophysiology - Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Abstract
Contamination of dental unit waterlines (DUWL) with heterotrophic bacteria can cause problems in immune compromised patients (aged, tumor and organ transplantation-patients). We focused on the use of low-concentrated ozonized water (OZW) as the biofilm formation restraint system for DUWL. Here, we examined the effects of low-concentrated OZW on the growth of bacteria and related biofilm formation and harmfulness to dental unit components (DUCs) in vitro.
Objectives
To evaluate the bactericidal effects of OZW on biofilms in DUWL and DUC in vitro.
Methods
Low-concentrated OZW (0.4 mg/L) was generated using an OZS-PTDX generator. Heterotrophic bacterial biofilms in old DUWL tubes and Candia albicans solution (control microbe) were treated with OZW for 1 h with gentle agitation before static culturing for 96 h in Reasoner's 2A liquid media. The control solutions were 0.1% cetylpyridinium chloride (CPC), chlorinated tap water (TW), and phosphate-buffered saline (PBS). Adenosine triphosphate (ATP) amounts of the microbes were measured and the biofilms of these microbes were observed using scanning electron microscopy (SEM). Moreover, surfaces of DUC soaked in OZW and TW were observed by SEM.
Results
The OZW reduced ATP levels in microbes to 50% compared to TW and PBS treatment, although CPC reduced it below detection limits. SEM observation revealed deformation of microbes cultured with OZW, whereas no changes were seen on DUC surfaces.
Conclusions
Low-concentrated OZW is bactericidal against heterotrophic bacteria biofilms and it is not harmful to DUC, suggesting that it might be useful in preventing DUWL contamination.
Keywords
Biofilm
Dental chair unit water line (DUWL)
Heterotrophic bacteria
Low-concentration
Materials science
Ozonized water
Published Date
2019-08-31
Publication Title
Heliyon
Volume
volume5
Issue
issue8
Publisher
Elsevier Science
Start Page
e02306
ISSN
24058440
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
© 2019 The Authors. Published by Elsevier Ltd.
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isVersionOf https://doi.org/10.1016/j.heliyon.2019.e02306
License
http://creativecommons.org/licenses/by/4.0/
Citation
Keisuke Okubo, Takashi Ito, Yasuyoshi Shiota, Yusuke Kawata, Tadashi Yamamoto, Shogo Takashiba, Effectiveness and safety of low-concentrated ozonized water for the reduction of contamination in dental unit water lines, Heliyon, Volume 5, Issue 8, 2019, e02306, ISSN 2405-8440, https://doi.org/10.1016/j.heliyon.2019.e02306.
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OA
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Non-OpenArchive