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Heliyon_5_6_01936.pdf 1.16 MB
Matsuo, Toshihiko Ophthalmology, Okayama University Medical School and Okayama University Hospital ORCID Kakenhi
Uchida, Tetsuya Polymer Materials Science, Okayama University Graduate School of Natural Science and Technology Kakenhi publons
Yamashita, Koichiro Polymer Materials Science, Okayama University Graduate School of Natural Science and Technology
Takei, Shigiko Ina Research
Ido, Daisuke Ina Research
Fujiwara, Atsushi Ina Research
Iino, Masahiko Ina Research
Oguchi, Masao Ina Research
Vision plays a key role in some behavior tests for rats. Okayama University-type retinal prosthesis (OUReP) is a photoelectric dye-coupled polyethylene film which generates electric potential in response to light and stimulates nearby neurons. This study aims to assess vision in retinal dystrophic (RCS) rats, in comparison with normal rats, by selected behavior tests. We also examined whether the tests could detect vision changes in RCS rats with dye-coupled film implantation.
Data sets were 5 normal rats, 4 untreated RCS rats, 7 RCS rats with dye-coupled films implanted at the age of 7 weeks after excluding unsuccessful implantation at autopsy. Behavior tests chosen were landing foot splay and visual forelimb-placing response in the menu of functional observational battery, operant-conditioning lever-press response and light/dark box test.
Normal visual placing response was significantly less frequent in untreated RCS rats at the age of 9 and 11 weeks, compared with normal rats (P = 0.0027, chi-square test) while normal response was significantly more frequent at the age of 9 weeks in RCS rats with dye-coupled film implantation, compared with untreated RCS rats (P = 0.0221). In operant-conditioning lever-press test, the correct response rate was significantly lower in untreated RCS rats than in normal rats at the age of 9 weeks (P < 0.05, Tukey-Kramer test) while the rate was not significantly different between normal rats and RCS rats with dye-coupled film implantation. In light/dark box test, the time to enter dark box was significantly shorter in normal rats, compared with untreated RCS rats or RCS rats with dye-coupled film implantation (P < 0.05, Tukey-Kramer test).
Behavior tests of functional observational battery, operant-conditioning lever-press response and light/dark box test discriminated vision between normal rats and RCS rats. The visual placing response and operant-conditioning lever-press test might have sensitivity to detect vision recovery in RCS rats with OUReP implantation.
© 2019 The Authors.
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Toshihiko Matsuo, Tetsuya Uchida, Koichiro Yamashita, Shigiko Takei, Daisuke Ido, Atsushi Fujiwara, Masahiko Iino, Masao Oguchi, Vision evaluation by functional observational battery, operant behavior test, and light/dark box test in retinal dystrophic RCS rats versus normal rats, Heliyon, Volume 5, Issue 6, 2019, e01936, ISSN 2405-8440, https://doi.org/10.1016/j.heliyon.2019.e01936.
Japan Agency for Medical Research and Development
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