ID | 62341 |
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Author |
Kawata, Kazumi
Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
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Narita, Keishi
Department of Anatomy and Cell Biology, University of Yamanashi Interdisciplinary Graduate School of Medicine and Engineering
Washio, Ayako
Division of Endodontics and Restorative Dentistry, Department of Oral Functions, Kyushu Dental University
Kitamura, Chiaki
Division of Endodontics and Restorative Dentistry, Department of Oral Functions, Kyushu Dental University
Nishihara, Tatsuji
Division of Infections and Molecular Biology, Department of Health Promotion, Kyushu Dental University,
Kubota, Satoshi
Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
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Takeda, Sen
Department of Anatomy and Cell Biology, University of Yamanashi Interdisciplinary Graduate School of Medicine and Engineering
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Abstract | Primary cilium is a protruding cellular organelle that has various physiological functions, especially in sensory reception. While an avalanche of reports on primary cilia have been published, the function of primary cilia in dental cells remains to be investigated. In this study, we focused on the function of primary cilia in dentin-producing odontoblasts. Odontoblasts, like most other cell types, possess primary cilia, which disappear upon the knockdown of intraflagellar transport-88. In cilia-depleted cells, the expression of dentin sialoprotein, an odontoblastic marker, was elevated, while the deposition of minerals was slowed. This was recapitulated by the activation of canonical Wnt pathway, also decreased the ratio of ciliated cells. In dental pulp cells, as they differentiated into odontoblasts, the ratio of ciliated cells was increased, whereas the canonical Wnt signaling activity was repressed. Our results collectively underscore the roles of primary cilia in regulating odontoblastic differentiation through canonical Wnt signaling. This study implies the existence of a feedback loop between primary cilia and the canonical Wnt pathway.
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Keywords | Primary cilia
IFT88
Odontoblast
Odontoblast differentiation
Canonical Wnt/β-catenin signaling pathway
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Note | This is an Accepted Manuscript of an article published by Elsevier.
© 2021 Elsevier Inc. This manuscript version is made available under the CC-BY-NC-ND 4.0 License. http://creativecommons.org/licenses/by-nc-nd/4.0/. This is the accepted manuscript version. The formal published version is available at https://doi.org/10.1016/j.bone.2021.116001 |
Published Date | 2021-9
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Publication Title |
Bone
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Volume | volume150
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Publisher | Elsevier BV
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Start Page | 116001
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ISSN | 8756-3282
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © 2021 Elsevier Inc. All rights reserved.
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File Version | author
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Related Url | isVersionOf https://doi.org/10.1016/j.bone.2021.116001
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License | http://creativecommons.org/licenses/by-nc-nd/4.0/
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Funder Name |
Japan Society for the Promotion of Science
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助成番号 | 26460271
25870277
24770183
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